Concentrations of thioredoxin, a redox-regulating protein, in umbilical cord blood and breast milk

Yukiko Todoroki, Hirokazu Tsukahara, Yusei Ohshima, Ken Ichi Shukunami, Koji Nishijima, Fumikazu Kotsuji, Atsuko Hata, Kenkou Kasuga, Kyouichi Sekine, Hajime Nakamura, Junji Yodoi, Mitsufumi Mayumi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Growing evidence indicates that oxidative stress occurs during the fetal-to-neonatal transition. Such stress plays an important role in the pathogenesis of many neonatal diseases. Thioredoxin (TRX), a redox-regulating protein with antioxidant activity, is induced in various cells against oxidative stress and is secreted extracellularly. This study was undertaken to examine the clinical and biological importance of TRX in the perinatal setting. We measured concentrations of TRX in umbilical cord blood and breast milk using a sandwich ELISA. Our study demonstrated that concentrations of TRX in umbilical cord blood were six to seven times higher than those in blood of healthy adults. This study also showed that umbilical concentrations of TRX were correlated significantly with the extent of prematurity of the newborn, and that they were elevated significantly in newborns of mothers with preeclampsia compared to those of mothers without preeclampsia. In contrast, concentrations of coenzyme Q10 and vitamin E in umbilical blood were lower than adult blood levels. Breast milk concentrations of TRX during the early postpartum period were seven to eight times higher than those in blood of lactating women. Those of the coenzyme Q10 were lower than adult blood levels, while those of vitamin E were comparable to adult blood levels. Our findings suggest that the systemic release of TRX is enhanced at birth, and that early breast milk is a rich source of this protein. Consequent high levels of TRX in newborns may provide a unique protective mechanism that allows the maintenance of redox balance during the fetal-to-neonatal transition.

Original languageEnglish
Pages (from-to)291-297
Number of pages7
JournalFree Radical Research
Volume39
Issue number3
DOIs
Publication statusPublished - Mar 1 2005
Externally publishedYes

Keywords

  • Breast milk
  • Newborn
  • Oxidative stress
  • Preeclampsia
  • Thioredoxin
  • Umbilical blood

ASJC Scopus subject areas

  • Biochemistry

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  • Cite this

    Todoroki, Y., Tsukahara, H., Ohshima, Y., Shukunami, K. I., Nishijima, K., Kotsuji, F., Hata, A., Kasuga, K., Sekine, K., Nakamura, H., Yodoi, J., & Mayumi, M. (2005). Concentrations of thioredoxin, a redox-regulating protein, in umbilical cord blood and breast milk. Free Radical Research, 39(3), 291-297. https://doi.org/10.1080/10715760500053578