Concentrations of pentosidine, an advanced glycation end-product, in umbilical cord blood

Hirokazu Tsukahara, Naoko Ohta, Shuko Sato, Masahiro Hiraoko, Ken Ichi Shukunami, Mayumi Uchiyama, Hisako Kawakami, Kyouichi Sekine, Mitsufumi Mayumi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Advanced glycation end-products (AGEs) are formed over several weeks to months by non-enzymatic glycation and oxidation ("glycoxidation") reactions between carbohydrate-derived carbonyl groups and protein amino groups, known as the Maillard reaction. Pentosidine is one of the best-characterized AGEs and is accepted as a satisfactory marker for glycoxidation in vivo. The present study was intended to measure pentosidine concentrations in umbilical cord blood from newborns with various gestational ages using our recently established high-performance liquid chromatography method [Tsukahara, H. et al. (2003) Pediatr. Res. 54, 419-424]. Our study demonstrates, for the first time, that pentosidine is detected in most of the umbilical blood samples. This study also shows that the umbilical blood concentrations of pentosidine are considerably lower than normal adult values, but that they increase with gestation progression and fetal growth. Umbilical pentosidine concentrations were significantly elevated in newborns of mothers with preeclampsia compared to those of mothers without preeclampsia. We conclude that accumulation of AGEs and oxidative stress occurs in fetal tissues and organs in utero at the early stage of human life and that their accumulation is augmented in the maternal preeclampsic condition.

Original languageEnglish
Pages (from-to)691-695
Number of pages5
JournalFree Radical Research
Volume38
Issue number7
DOIs
Publication statusPublished - Jul 2004
Externally publishedYes

Fingerprint

Advanced Glycosylation End Products
Fetal Blood
Blood
Umbilicus
Pre-Eclampsia
Maillard Reaction
Oxidative stress
High performance liquid chromatography
Fetal Development
Gestational Age
Reference Values
Oxidative Stress
Fetus
High Pressure Liquid Chromatography
Mothers
Carbohydrates
pentosidine
Tissue
Pregnancy
Oxidation

Keywords

  • Advanced glycation end-product
  • Newborn
  • Pentosidine
  • Preeclampsia
  • Umbilical blood

ASJC Scopus subject areas

  • Biochemistry

Cite this

Concentrations of pentosidine, an advanced glycation end-product, in umbilical cord blood. / Tsukahara, Hirokazu; Ohta, Naoko; Sato, Shuko; Hiraoko, Masahiro; Shukunami, Ken Ichi; Uchiyama, Mayumi; Kawakami, Hisako; Sekine, Kyouichi; Mayumi, Mitsufumi.

In: Free Radical Research, Vol. 38, No. 7, 07.2004, p. 691-695.

Research output: Contribution to journalArticle

Tsukahara, H, Ohta, N, Sato, S, Hiraoko, M, Shukunami, KI, Uchiyama, M, Kawakami, H, Sekine, K & Mayumi, M 2004, 'Concentrations of pentosidine, an advanced glycation end-product, in umbilical cord blood', Free Radical Research, vol. 38, no. 7, pp. 691-695. https://doi.org/10.1080/1071576042000220256
Tsukahara, Hirokazu ; Ohta, Naoko ; Sato, Shuko ; Hiraoko, Masahiro ; Shukunami, Ken Ichi ; Uchiyama, Mayumi ; Kawakami, Hisako ; Sekine, Kyouichi ; Mayumi, Mitsufumi. / Concentrations of pentosidine, an advanced glycation end-product, in umbilical cord blood. In: Free Radical Research. 2004 ; Vol. 38, No. 7. pp. 691-695.
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