Concentrated expression of Ca2+/calmodulin-dependent protein kinase II and protein kinase C in the mushroom bodies of the brain of the honeybee Apis mellifera L.

Azusa Kamikouchi, Hideaki Takeuchi, Miyuki Sawata, Shunji Natori, Takeo Kubo

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

We have previously used the differential display method to identify a gene that is expressed preferentially in the mushroom bodies of worker honeybees and to show that it encodes a putative inositol 1,4,5-trisphosphate receptor (IP3R) homologue (Kamikouchi et al. [1998] Biochem. Biophys. Res. Commun. 242:181-186). In the present study, we examined whether the expression of some of the genes for proteins involved in the intracellular Ca2+ signal transduction is also concentrated in the mushroom bodies of the honeybee by isolating cDNA fragments that encode the Ca2+/calmodulin- dependent protein kinase II (CaMKII) and protein kinase C (PKC) homologues of the honeybee. In situ hybridization analysis revealed that the expression of these genes was also concentrated in the mushroom bodies of the honeybee brain: The CaMKII gene was expressed preferentially in the large-type Kenyon cells of the mushroom bodies, whereas that for PKC was expressed in both the large and small types of Kenyon cells. The expression of the genes for IP3R and CaMKII was concentrated in the mushroom bodies of the queen and drone as well as in those of the worker bee. Furthermore, the enzymatic activities of CaMKII and PKC were found to be higher in the mushroom bodies/central bodies than in the optic and antennal lobes of the worker bee brain. These results suggest that the function of the intracellular Ca2+ signal transduction is enhanced in Kenyon cells in comparison to other neuronal cell types in the honeybee brain. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)501-510
Number of pages10
JournalJournal of Comparative Neurology
Volume417
Issue number4
DOIs
Publication statusPublished - Feb 21 2000
Externally publishedYes

Fingerprint

Mushroom Bodies
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Bees
Protein Kinase C
Brain
Signal Transduction
Inositol 1,4,5-Trisphosphate Receptors
Gene Expression
Genes
In Situ Hybridization
Complementary DNA

Keywords

  • cDNA cloning
  • Gene expression
  • In situ hybridization
  • Intracellular Ca signal transduction
  • Kenyon cells

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Concentrated expression of Ca2+/calmodulin-dependent protein kinase II and protein kinase C in the mushroom bodies of the brain of the honeybee Apis mellifera L. / Kamikouchi, Azusa; Takeuchi, Hideaki; Sawata, Miyuki; Natori, Shunji; Kubo, Takeo.

In: Journal of Comparative Neurology, Vol. 417, No. 4, 21.02.2000, p. 501-510.

Research output: Contribution to journalArticle

@article{7d31548d02ae450a939e38ea93ebc5d5,
title = "Concentrated expression of Ca2+/calmodulin-dependent protein kinase II and protein kinase C in the mushroom bodies of the brain of the honeybee Apis mellifera L.",
abstract = "We have previously used the differential display method to identify a gene that is expressed preferentially in the mushroom bodies of worker honeybees and to show that it encodes a putative inositol 1,4,5-trisphosphate receptor (IP3R) homologue (Kamikouchi et al. [1998] Biochem. Biophys. Res. Commun. 242:181-186). In the present study, we examined whether the expression of some of the genes for proteins involved in the intracellular Ca2+ signal transduction is also concentrated in the mushroom bodies of the honeybee by isolating cDNA fragments that encode the Ca2+/calmodulin- dependent protein kinase II (CaMKII) and protein kinase C (PKC) homologues of the honeybee. In situ hybridization analysis revealed that the expression of these genes was also concentrated in the mushroom bodies of the honeybee brain: The CaMKII gene was expressed preferentially in the large-type Kenyon cells of the mushroom bodies, whereas that for PKC was expressed in both the large and small types of Kenyon cells. The expression of the genes for IP3R and CaMKII was concentrated in the mushroom bodies of the queen and drone as well as in those of the worker bee. Furthermore, the enzymatic activities of CaMKII and PKC were found to be higher in the mushroom bodies/central bodies than in the optic and antennal lobes of the worker bee brain. These results suggest that the function of the intracellular Ca2+ signal transduction is enhanced in Kenyon cells in comparison to other neuronal cell types in the honeybee brain. (C) 2000 Wiley-Liss, Inc.",
keywords = "cDNA cloning, Gene expression, In situ hybridization, Intracellular Ca signal transduction, Kenyon cells",
author = "Azusa Kamikouchi and Hideaki Takeuchi and Miyuki Sawata and Shunji Natori and Takeo Kubo",
year = "2000",
month = "2",
day = "21",
doi = "10.1002/(SICI)1096-9861(20000221)417:4<501::AID-CNE8>3.0.CO;2-4",
language = "English",
volume = "417",
pages = "501--510",
journal = "Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Concentrated expression of Ca2+/calmodulin-dependent protein kinase II and protein kinase C in the mushroom bodies of the brain of the honeybee Apis mellifera L.

AU - Kamikouchi, Azusa

AU - Takeuchi, Hideaki

AU - Sawata, Miyuki

AU - Natori, Shunji

AU - Kubo, Takeo

PY - 2000/2/21

Y1 - 2000/2/21

N2 - We have previously used the differential display method to identify a gene that is expressed preferentially in the mushroom bodies of worker honeybees and to show that it encodes a putative inositol 1,4,5-trisphosphate receptor (IP3R) homologue (Kamikouchi et al. [1998] Biochem. Biophys. Res. Commun. 242:181-186). In the present study, we examined whether the expression of some of the genes for proteins involved in the intracellular Ca2+ signal transduction is also concentrated in the mushroom bodies of the honeybee by isolating cDNA fragments that encode the Ca2+/calmodulin- dependent protein kinase II (CaMKII) and protein kinase C (PKC) homologues of the honeybee. In situ hybridization analysis revealed that the expression of these genes was also concentrated in the mushroom bodies of the honeybee brain: The CaMKII gene was expressed preferentially in the large-type Kenyon cells of the mushroom bodies, whereas that for PKC was expressed in both the large and small types of Kenyon cells. The expression of the genes for IP3R and CaMKII was concentrated in the mushroom bodies of the queen and drone as well as in those of the worker bee. Furthermore, the enzymatic activities of CaMKII and PKC were found to be higher in the mushroom bodies/central bodies than in the optic and antennal lobes of the worker bee brain. These results suggest that the function of the intracellular Ca2+ signal transduction is enhanced in Kenyon cells in comparison to other neuronal cell types in the honeybee brain. (C) 2000 Wiley-Liss, Inc.

AB - We have previously used the differential display method to identify a gene that is expressed preferentially in the mushroom bodies of worker honeybees and to show that it encodes a putative inositol 1,4,5-trisphosphate receptor (IP3R) homologue (Kamikouchi et al. [1998] Biochem. Biophys. Res. Commun. 242:181-186). In the present study, we examined whether the expression of some of the genes for proteins involved in the intracellular Ca2+ signal transduction is also concentrated in the mushroom bodies of the honeybee by isolating cDNA fragments that encode the Ca2+/calmodulin- dependent protein kinase II (CaMKII) and protein kinase C (PKC) homologues of the honeybee. In situ hybridization analysis revealed that the expression of these genes was also concentrated in the mushroom bodies of the honeybee brain: The CaMKII gene was expressed preferentially in the large-type Kenyon cells of the mushroom bodies, whereas that for PKC was expressed in both the large and small types of Kenyon cells. The expression of the genes for IP3R and CaMKII was concentrated in the mushroom bodies of the queen and drone as well as in those of the worker bee. Furthermore, the enzymatic activities of CaMKII and PKC were found to be higher in the mushroom bodies/central bodies than in the optic and antennal lobes of the worker bee brain. These results suggest that the function of the intracellular Ca2+ signal transduction is enhanced in Kenyon cells in comparison to other neuronal cell types in the honeybee brain. (C) 2000 Wiley-Liss, Inc.

KW - cDNA cloning

KW - Gene expression

KW - In situ hybridization

KW - Intracellular Ca signal transduction

KW - Kenyon cells

UR - http://www.scopus.com/inward/record.url?scp=0034695747&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034695747&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1096-9861(20000221)417:4<501::AID-CNE8>3.0.CO;2-4

DO - 10.1002/(SICI)1096-9861(20000221)417:4<501::AID-CNE8>3.0.CO;2-4

M3 - Article

C2 - 10701869

AN - SCOPUS:0034695747

VL - 417

SP - 501

EP - 510

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 4

ER -