Computerized approach using autoradiography to quantify atrial natriuretic peptide receptors in the DOCA-salt hypertensive rat kidney

Katsuhiko Takatori, Toshio Ogura, Zensuke Ota

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The alteration of atrial natriuretic peptide (ANP) receptors was investigated in the kidney of deoxycorticosterone acetate (DOCA)-salt treated hypertensive rats. The absolute amount of renal ANP receptors was determined in a membrane homogenate binding study of rat whole kidneys. Administration of DOCA-salt led to a decrease in renal ANP receptors after 3 weeks (prehypertensive state) and 6 weeks (established hypertensive state) of treatment. In vitro macro-autoradiography (ARG) was then performed with [125I]ANP to localize and to quantitate specific renal ANP receptors. ARG revealed that specific ANP binding was distributed mainly over the renal cortex with the inner medulla next in frequency. Renal ANP receptors were therefore quantified over the cortex and the inner medulla using the computerized microdensitometry of ARG. A significant reduction in renal ANP receptors was observed in the DOCA-salt treated rats after 3 and 6 weeks of treatment with decrements observed in both the cortex and inner medulla. These alterations may be related to the pathophysiology of hypertension.

Original languageEnglish
Pages (from-to)115-125
Number of pages11
JournalRegulatory Peptides
Volume35
Issue number2
DOIs
Publication statusPublished - Aug 13 1991

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Keywords

  • Atrial natriuretic peptide
  • Autoradiography
  • Computerized microdensitometry
  • DOCA-salt hypertensive rat
  • Kidney
  • Receptor

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

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