TY - JOUR
T1 - Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries
AU - Matsumoto, Takashi
AU - Tanaka, Tsuyoshi
AU - Sakai, Hiroaki
AU - Amano, Naoki
AU - Kanamori, Hiroyuki
AU - Kurita, Kanako
AU - Kikuta, Ari
AU - Kamiya, Kozue
AU - Yamamoto, Mayu
AU - Ikawa, Hiroshi
AU - Fujii, Nobuyuki
AU - Hori, Kiyosumi
AU - Itoh, Takeshi
AU - Sato, Kazuhiro
PY - 2011/5
Y1 - 2011/5
N2 - Full-length cDNA (FLcDNA) libraries consisting of 172,000 clones were constructed from a two-row malting barley cultivar (Hordeum vulgare 'Haruna Nijo') under normal and stressed conditions. After sequencing the clones from both ends and clustering the sequences, a total of 24,783 complete sequences were produced. By removing duplicates between these and publicly available sequences, 22,651 representative sequences were obtained: 17,773 were novel barley FLcDNAs, and 1,699 were barley specific. Highly conserved genes were found in the barley FLcDNA sequences for 721 of 881 rice (Oryza sativa) trait genes with 50% or greater identity. These FLcDNA resources from our Haruna Nijo cDNA libraries and the full-length sequences of representative clones will improve our understanding of the biological functions of genes in barley, which is the cereal crop with the fourth highest production in the world, and will provide a powerful tool for annotating the barley genome sequences that will become available in the near future.
AB - Full-length cDNA (FLcDNA) libraries consisting of 172,000 clones were constructed from a two-row malting barley cultivar (Hordeum vulgare 'Haruna Nijo') under normal and stressed conditions. After sequencing the clones from both ends and clustering the sequences, a total of 24,783 complete sequences were produced. By removing duplicates between these and publicly available sequences, 22,651 representative sequences were obtained: 17,773 were novel barley FLcDNAs, and 1,699 were barley specific. Highly conserved genes were found in the barley FLcDNA sequences for 721 of 881 rice (Oryza sativa) trait genes with 50% or greater identity. These FLcDNA resources from our Haruna Nijo cDNA libraries and the full-length sequences of representative clones will improve our understanding of the biological functions of genes in barley, which is the cereal crop with the fourth highest production in the world, and will provide a powerful tool for annotating the barley genome sequences that will become available in the near future.
UR - http://www.scopus.com/inward/record.url?scp=79956012836&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79956012836&partnerID=8YFLogxK
U2 - 10.1104/pp.110.171579
DO - 10.1104/pp.110.171579
M3 - Article
C2 - 21415278
AN - SCOPUS:79956012836
SN - 0032-0889
VL - 156
SP - 20
EP - 28
JO - Plant Physiology
JF - Plant Physiology
IS - 1
ER -