Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries

Takashi Matsumoto, Tsuyoshi Tanaka, Hiroaki Sakai, Naoki Amano, Hiroyuki Kanamori, Kanako Kurita, Ari Kikuta, Kozue Kamiya, Mayu Yamamoto, Hiroshi Ikawa, Nobuyuki Fujii, Kiyosumi Hori, Takeshi Itoh, Kazuhiro Sato

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

Full-length cDNA (FLcDNA) libraries consisting of 172,000 clones were constructed from a two-row malting barley cultivar (Hordeum vulgare 'Haruna Nijo') under normal and stressed conditions. After sequencing the clones from both ends and clustering the sequences, a total of 24,783 complete sequences were produced. By removing duplicates between these and publicly available sequences, 22,651 representative sequences were obtained: 17,773 were novel barley FLcDNAs, and 1,699 were barley specific. Highly conserved genes were found in the barley FLcDNA sequences for 721 of 881 rice (Oryza sativa) trait genes with 50% or greater identity. These FLcDNA resources from our Haruna Nijo cDNA libraries and the full-length sequences of representative clones will improve our understanding of the biological functions of genes in barley, which is the cereal crop with the fourth highest production in the world, and will provide a powerful tool for annotating the barley genome sequences that will become available in the near future.

Original languageEnglish
Pages (from-to)20-28
Number of pages9
JournalPlant Physiology
Volume156
Issue number1
DOIs
Publication statusPublished - May 2011

    Fingerprint

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology
  • Medicine(all)

Cite this

Matsumoto, T., Tanaka, T., Sakai, H., Amano, N., Kanamori, H., Kurita, K., Kikuta, A., Kamiya, K., Yamamoto, M., Ikawa, H., Fujii, N., Hori, K., Itoh, T., & Sato, K. (2011). Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries. Plant Physiology, 156(1), 20-28. https://doi.org/10.1104/pp.110.171579