Abstract
Emerging work has suggested the existence of phytohormones in seaweeds, although chemical species, endogenous biosynthetic pathways, and signal transduction machineries remain poorly understood. We performed profiling of nine phytohormones with liquid chromatography-mass spectrometry and in silico genome-wide homology search to identify genes involved in biosynthesis and signal transduction of hormones in red algae. It was demonstrated that two Bangiophycean algae, Bangia fuscopurpurea and Pyropia yezoensis, possessed indoleacetic acid (IAA), N6-(Δ2-isopentenyl)adenine (iP), abscisic acid (ABA), and salicylic acid, although trans-zeatin, dihydrozeatin, gibberellin A1 and A4, and jasmonate were not detected. Results of genome-wide survey demonstrated that Bangiophycean algae produce iP and ABA via pathways similar to those in terrestrial plants. However, these seaweeds lack homologues of already known factors participating in perception and signal transduction of IAA, iP, ABA and SA, indicating that the action modes of these phytohormones in red seaweeds differ from those elucidated in terrestrial plants. These findings shed lights on evolutional divergence of signal transduction pathways of phytohormones in plants.
| Original language | English |
|---|---|
| Article number | 016 |
| Pages (from-to) | 2539-2548 |
| Number of pages | 10 |
| Journal | Journal of Applied Phycology |
| Volume | 28 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Aug 1 2016 |
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Keywords
- Bangia fuscopurpurea
- Comparative genomics
- Liquid chromatography-tandemmass spectrometry
- Phytohormone
- Pyropia yezoensis
- Quantitative profiling
ASJC Scopus subject areas
- Aquatic Science
- Plant Science
Cite this
Comprehensive quantification and genome survey reveal the presence of novel phytohormone action modes in red seaweeds. / Mikami, Koji; Mori, Izumi; Matsuura, Takakazu; Ikeda, Yoko; Kojima, Mikiko; Sakakibara, Hitoshi; Hirayama, Takashi.
In: Journal of Applied Phycology, Vol. 28, No. 4, 016, 01.08.2016, p. 2539-2548.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comprehensive quantification and genome survey reveal the presence of novel phytohormone action modes in red seaweeds
AU - Mikami, Koji
AU - Mori, Izumi
AU - Matsuura, Takakazu
AU - Ikeda, Yoko
AU - Kojima, Mikiko
AU - Sakakibara, Hitoshi
AU - Hirayama, Takashi
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Emerging work has suggested the existence of phytohormones in seaweeds, although chemical species, endogenous biosynthetic pathways, and signal transduction machineries remain poorly understood. We performed profiling of nine phytohormones with liquid chromatography-mass spectrometry and in silico genome-wide homology search to identify genes involved in biosynthesis and signal transduction of hormones in red algae. It was demonstrated that two Bangiophycean algae, Bangia fuscopurpurea and Pyropia yezoensis, possessed indoleacetic acid (IAA), N6-(Δ2-isopentenyl)adenine (iP), abscisic acid (ABA), and salicylic acid, although trans-zeatin, dihydrozeatin, gibberellin A1 and A4, and jasmonate were not detected. Results of genome-wide survey demonstrated that Bangiophycean algae produce iP and ABA via pathways similar to those in terrestrial plants. However, these seaweeds lack homologues of already known factors participating in perception and signal transduction of IAA, iP, ABA and SA, indicating that the action modes of these phytohormones in red seaweeds differ from those elucidated in terrestrial plants. These findings shed lights on evolutional divergence of signal transduction pathways of phytohormones in plants.
AB - Emerging work has suggested the existence of phytohormones in seaweeds, although chemical species, endogenous biosynthetic pathways, and signal transduction machineries remain poorly understood. We performed profiling of nine phytohormones with liquid chromatography-mass spectrometry and in silico genome-wide homology search to identify genes involved in biosynthesis and signal transduction of hormones in red algae. It was demonstrated that two Bangiophycean algae, Bangia fuscopurpurea and Pyropia yezoensis, possessed indoleacetic acid (IAA), N6-(Δ2-isopentenyl)adenine (iP), abscisic acid (ABA), and salicylic acid, although trans-zeatin, dihydrozeatin, gibberellin A1 and A4, and jasmonate were not detected. Results of genome-wide survey demonstrated that Bangiophycean algae produce iP and ABA via pathways similar to those in terrestrial plants. However, these seaweeds lack homologues of already known factors participating in perception and signal transduction of IAA, iP, ABA and SA, indicating that the action modes of these phytohormones in red seaweeds differ from those elucidated in terrestrial plants. These findings shed lights on evolutional divergence of signal transduction pathways of phytohormones in plants.
KW - Bangia fuscopurpurea
KW - Comparative genomics
KW - Liquid chromatography-tandemmass spectrometry
KW - Phytohormone
KW - Pyropia yezoensis
KW - Quantitative profiling
UR - http://www.scopus.com/inward/record.url?scp=85032076703&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85032076703&partnerID=8YFLogxK
U2 - 10.1007/s10811-015-0759-2
DO - 10.1007/s10811-015-0759-2
M3 - Article
AN - SCOPUS:85032076703
VL - 28
SP - 2539
EP - 2548
JO - Journal of Applied Phycology
JF - Journal of Applied Phycology
SN - 0921-8971
IS - 4
M1 - 016
ER -