Comprehensive quantification and genome survey reveal the presence of novel phytohormone action modes in red seaweeds

Koji Mikami, Izumi Mori, Takakazu Matsuura, Yoko Ikeda, Mikiko Kojima, Hitoshi Sakakibara, Takashi Hirayama

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Emerging work has suggested the existence of phytohormones in seaweeds, although chemical species, endogenous biosynthetic pathways, and signal transduction machineries remain poorly understood. We performed profiling of nine phytohormones with liquid chromatography-mass spectrometry and in silico genome-wide homology search to identify genes involved in biosynthesis and signal transduction of hormones in red algae. It was demonstrated that two Bangiophycean algae, Bangia fuscopurpurea and Pyropia yezoensis, possessed indoleacetic acid (IAA), N6-(Δ2-isopentenyl)adenine (iP), abscisic acid (ABA), and salicylic acid, although trans-zeatin, dihydrozeatin, gibberellin A1 and A4, and jasmonate were not detected. Results of genome-wide survey demonstrated that Bangiophycean algae produce iP and ABA via pathways similar to those in terrestrial plants. However, these seaweeds lack homologues of already known factors participating in perception and signal transduction of IAA, iP, ABA and SA, indicating that the action modes of these phytohormones in red seaweeds differ from those elucidated in terrestrial plants. These findings shed lights on evolutional divergence of signal transduction pathways of phytohormones in plants.

Original languageEnglish
Article number016
Pages (from-to)2539-2548
Number of pages10
JournalJournal of Applied Phycology
Volume28
Issue number4
DOIs
Publication statusPublished - Aug 1 2016

Fingerprint

seaweed
algae
plant hormones
abscisic acid
macroalgae
signal transduction
mechanism of action
adenine
genome
indole acetic acid
Bangia
alga
dihydrozeatin
salicylic acid
chemical speciation
acid
red alga
zeatin
homology
gibberellins

Keywords

  • Bangia fuscopurpurea
  • Comparative genomics
  • Liquid chromatography-tandemmass spectrometry
  • Phytohormone
  • Pyropia yezoensis
  • Quantitative profiling

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Cite this

Comprehensive quantification and genome survey reveal the presence of novel phytohormone action modes in red seaweeds. / Mikami, Koji; Mori, Izumi; Matsuura, Takakazu; Ikeda, Yoko; Kojima, Mikiko; Sakakibara, Hitoshi; Hirayama, Takashi.

In: Journal of Applied Phycology, Vol. 28, No. 4, 016, 01.08.2016, p. 2539-2548.

Research output: Contribution to journalArticle

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