Abstract
Objectives: We sought to assess loss of heterozygosity (LOH) profiles of 3p, 6q, 8q, 10q, 12q, 13q, and 17p and to identify the tumor suppressor genes involved in salivary gland neoplasms. Study Design: LOH analysis was performed using 26 microsatellite markers by polymerase chain reaction-polyacrylamide gel electrophoresis method in 20 benign and 6 malignant salivary gland tumors. Results: Overall, LOH was detected in at least one informative locus in 18 of 20 (90%) of benign tumors and in all of 6 cases of malignant tumors. High LOH frequencies were revealed at the loci D3S1307 (22%, 3p26), D3S966 (41%, 3p21), D6S255 (27%, 6q25), D8S166 (25%, 8q12), D8S199 (21%, 8q24), and D10S1765 (28%, 10q23) in benign tumors, defining the hotspot regions for putative tumor suppressor genes. Conclusions and Significance: The hotspot regions defined by the present study suggest that new tumor suppressor genes related to the development of salivary gland tumors may reside at several chromosomal loci, including loci at 3p, 6q, 8q and 10q.
| Original language | English |
|---|---|
| Pages (from-to) | 119-125 |
| Number of pages | 7 |
| Journal | Otolaryngology - Head and Neck Surgery |
| Volume | 137 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jul 2007 |
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ASJC Scopus subject areas
- Otorhinolaryngology
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Comprehensive loss of heterozygosity analysis and identification of a novel hotspot at 3p21 in salivary gland neoplasms. / Honjo, Noriyasu; Gunduz, Mehmet; Fukushima, Kunihiro; Cengiz, Beyhan; Beder, Levent B.; Gunduz, Esra; Nagatsuka, Hitoshi; Xiao, Jing; Shimizu, Kenji; Nishizaki, Kazunori.
In: Otolaryngology - Head and Neck Surgery, Vol. 137, No. 1, 07.2007, p. 119-125.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comprehensive loss of heterozygosity analysis and identification of a novel hotspot at 3p21 in salivary gland neoplasms
AU - Honjo, Noriyasu
AU - Gunduz, Mehmet
AU - Fukushima, Kunihiro
AU - Cengiz, Beyhan
AU - Beder, Levent B.
AU - Gunduz, Esra
AU - Nagatsuka, Hitoshi
AU - Xiao, Jing
AU - Shimizu, Kenji
AU - Nishizaki, Kazunori
PY - 2007/7
Y1 - 2007/7
N2 - Objectives: We sought to assess loss of heterozygosity (LOH) profiles of 3p, 6q, 8q, 10q, 12q, 13q, and 17p and to identify the tumor suppressor genes involved in salivary gland neoplasms. Study Design: LOH analysis was performed using 26 microsatellite markers by polymerase chain reaction-polyacrylamide gel electrophoresis method in 20 benign and 6 malignant salivary gland tumors. Results: Overall, LOH was detected in at least one informative locus in 18 of 20 (90%) of benign tumors and in all of 6 cases of malignant tumors. High LOH frequencies were revealed at the loci D3S1307 (22%, 3p26), D3S966 (41%, 3p21), D6S255 (27%, 6q25), D8S166 (25%, 8q12), D8S199 (21%, 8q24), and D10S1765 (28%, 10q23) in benign tumors, defining the hotspot regions for putative tumor suppressor genes. Conclusions and Significance: The hotspot regions defined by the present study suggest that new tumor suppressor genes related to the development of salivary gland tumors may reside at several chromosomal loci, including loci at 3p, 6q, 8q and 10q.
AB - Objectives: We sought to assess loss of heterozygosity (LOH) profiles of 3p, 6q, 8q, 10q, 12q, 13q, and 17p and to identify the tumor suppressor genes involved in salivary gland neoplasms. Study Design: LOH analysis was performed using 26 microsatellite markers by polymerase chain reaction-polyacrylamide gel electrophoresis method in 20 benign and 6 malignant salivary gland tumors. Results: Overall, LOH was detected in at least one informative locus in 18 of 20 (90%) of benign tumors and in all of 6 cases of malignant tumors. High LOH frequencies were revealed at the loci D3S1307 (22%, 3p26), D3S966 (41%, 3p21), D6S255 (27%, 6q25), D8S166 (25%, 8q12), D8S199 (21%, 8q24), and D10S1765 (28%, 10q23) in benign tumors, defining the hotspot regions for putative tumor suppressor genes. Conclusions and Significance: The hotspot regions defined by the present study suggest that new tumor suppressor genes related to the development of salivary gland tumors may reside at several chromosomal loci, including loci at 3p, 6q, 8q and 10q.
UR - http://www.scopus.com/inward/record.url?scp=34250761905&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34250761905&partnerID=8YFLogxK
U2 - 10.1016/j.otohns.2007.01.017
DO - 10.1016/j.otohns.2007.01.017
M3 - Article
C2 - 17599578
AN - SCOPUS:34250761905
VL - 137
SP - 119
EP - 125
JO - Otolaryngology - Head and Neck Surgery
JF - Otolaryngology - Head and Neck Surgery
SN - 0194-5998
IS - 1
ER -