Comprehensive loss of heterozygosity analysis and identification of a novel hotspot at 3p21 in salivary gland neoplasms

Noriyasu Honjo, Mehmet Gunduz, Kunihiro Fukushima, Beyhan Cengiz, Levent B. Beder, Esra Gunduz, Hitoshi Nagatsuka, Jing Xiao, Kenji Shimizu, Kazunori Nishizaki

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Abstract

Objectives: We sought to assess loss of heterozygosity (LOH) profiles of 3p, 6q, 8q, 10q, 12q, 13q, and 17p and to identify the tumor suppressor genes involved in salivary gland neoplasms. Study Design: LOH analysis was performed using 26 microsatellite markers by polymerase chain reaction-polyacrylamide gel electrophoresis method in 20 benign and 6 malignant salivary gland tumors. Results: Overall, LOH was detected in at least one informative locus in 18 of 20 (90%) of benign tumors and in all of 6 cases of malignant tumors. High LOH frequencies were revealed at the loci D3S1307 (22%, 3p26), D3S966 (41%, 3p21), D6S255 (27%, 6q25), D8S166 (25%, 8q12), D8S199 (21%, 8q24), and D10S1765 (28%, 10q23) in benign tumors, defining the hotspot regions for putative tumor suppressor genes. Conclusions and Significance: The hotspot regions defined by the present study suggest that new tumor suppressor genes related to the development of salivary gland tumors may reside at several chromosomal loci, including loci at 3p, 6q, 8q and 10q.

Original languageEnglish
Pages (from-to)119-125
Number of pages7
JournalOtolaryngology - Head and Neck Surgery
Volume137
Issue number1
DOIs
Publication statusPublished - Jul 2007

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Salivary Gland Neoplasms
Loss of Heterozygosity
Tumor Suppressor Genes
Glandular and Epithelial Neoplasms
Neoplasms
Microsatellite Repeats
Polyacrylamide Gel Electrophoresis
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Otorhinolaryngology

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Comprehensive loss of heterozygosity analysis and identification of a novel hotspot at 3p21 in salivary gland neoplasms. / Honjo, Noriyasu; Gunduz, Mehmet; Fukushima, Kunihiro; Cengiz, Beyhan; Beder, Levent B.; Gunduz, Esra; Nagatsuka, Hitoshi; Xiao, Jing; Shimizu, Kenji; Nishizaki, Kazunori.

In: Otolaryngology - Head and Neck Surgery, Vol. 137, No. 1, 07.2007, p. 119-125.

Research output: Contribution to journalArticle

Honjo, Noriyasu ; Gunduz, Mehmet ; Fukushima, Kunihiro ; Cengiz, Beyhan ; Beder, Levent B. ; Gunduz, Esra ; Nagatsuka, Hitoshi ; Xiao, Jing ; Shimizu, Kenji ; Nishizaki, Kazunori. / Comprehensive loss of heterozygosity analysis and identification of a novel hotspot at 3p21 in salivary gland neoplasms. In: Otolaryngology - Head and Neck Surgery. 2007 ; Vol. 137, No. 1. pp. 119-125.
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AU - Beder, Levent B.

AU - Gunduz, Esra

AU - Nagatsuka, Hitoshi

AU - Xiao, Jing

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