Comprehensive analysis of glucan elicitor-regulated gene expression in tobacco BY-2 cells reveals a novel MYB transcription factor involved in the regulation of phenylpropanoid metabolism

Tomonori Shinya, Ivan Gális, Tomoko Narisawa, Mami Sasaki, Hiroo Fukuda, Hideaki Matsuoka, Mikako Saito, Ken Matsuoka

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We previously demonstrated that a β-1,3-, 1,6-oligoglucan (AaGlucan) from the fungus Alternaria alternata 102 shows strong elicitor activity in tobacco BY-2 cells. We have used cDNA microarray analysis to monitor global changes in gene expression in tobacco cells treated with this A. alternata fraction or with laminarin. In total, we identified 265 genes that were induced 1 h after treatment with an AaGlucan-enriched fraction or laminarin. Among them, we characterized in detail a novel tobacco R2R3 MYB-type transcription factor homolog (NtMYBGR1) and two DC1 domain-containing genes (NtDC1A and NtDC1B). Microarray data, together with overexpression and metabolic analyses, indicated that NtMYBGR1, but not the NtDC1 proteins, primarily targets the phenylpropanoid synthesis-related genes PAL and 4CL. These results suggest that NtMYBGR1 specifically regulates defense responses in BY-2 cells by enhancing phenylpropanoid metabolism in response to AaGlucan and laminarin elicitors.

Original languageEnglish
Pages (from-to)1404-1413
Number of pages10
JournalPlant and Cell Physiology
Volume48
Issue number10
DOIs
Publication statusPublished - Oct 1 2007
Externally publishedYes

Keywords

  • BY-2 cells
  • Gene expression
  • Glucan elicitor
  • MYB
  • Microarray
  • Phenylpropanoid pathway

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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