Comprehensive analysis of glucan elicitor-regulated gene expression in tobacco BY-2 cells reveals a novel MYB transcription factor involved in the regulation of phenylpropanoid metabolism

Tomonori Shinya; Ivan Gális; Tomoko Narisawa; Mami Sasaki; Hiroo Fukuda; Hideaki Matsuoka; Mikako Saito; Ken Matsuoka

doi:10.1093/pcp/pcm115

Comprehensive analysis of glucan elicitor-regulated gene expression in tobacco BY-2 cells reveals a novel MYB transcription factor involved in the regulation of phenylpropanoid metabolism

Tomonori Shinya, Ivan Gális, Tomoko Narisawa, Mami Sasaki, Hiroo Fukuda, Hideaki Matsuoka, Mikako Saito, Ken Matsuoka

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

We previously demonstrated that a β-1,3-, 1,6-oligoglucan (AaGlucan) from the fungus Alternaria alternata 102 shows strong elicitor activity in tobacco BY-2 cells. We have used cDNA microarray analysis to monitor global changes in gene expression in tobacco cells treated with this A. alternata fraction or with laminarin. In total, we identified 265 genes that were induced 1 h after treatment with an AaGlucan-enriched fraction or laminarin. Among them, we characterized in detail a novel tobacco R2R3 MYB-type transcription factor homolog (NtMYBGR1) and two DC1 domain-containing genes (NtDC1A and NtDC1B). Microarray data, together with overexpression and metabolic analyses, indicated that NtMYBGR1, but not the NtDC1 proteins, primarily targets the phenylpropanoid synthesis-related genes PAL and 4CL. These results suggest that NtMYBGR1 specifically regulates defense responses in BY-2 cells by enhancing phenylpropanoid metabolism in response to AaGlucan and laminarin elicitors.

Original language	English
Pages (from-to)	1404-1413
Number of pages	10
Journal	Plant and Cell Physiology
Volume	48
Issue number	10
DOIs	https://doi.org/10.1093/pcp/pcm115
Publication status	Published - Oct 2007
Externally published	Yes

Keywords

BY-2 cells
Gene expression
Glucan elicitor
MYB
Microarray
Phenylpropanoid pathway

ASJC Scopus subject areas

Physiology
Plant Science
Cell Biology

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Shinya, T., Gális, I., Narisawa, T., Sasaki, M., Fukuda, H., Matsuoka, H., Saito, M., & Matsuoka, K. (2007). Comprehensive analysis of glucan elicitor-regulated gene expression in tobacco BY-2 cells reveals a novel MYB transcription factor involved in the regulation of phenylpropanoid metabolism. Plant and Cell Physiology, 48(10), 1404-1413. https://doi.org/10.1093/pcp/pcm115

Comprehensive analysis of glucan elicitor-regulated gene expression in tobacco BY-2 cells reveals a novel MYB transcription factor involved in the regulation of phenylpropanoid metabolism. / Shinya, Tomonori ; Gális, Ivan; Narisawa, Tomoko; Sasaki, Mami; Fukuda, Hiroo; Matsuoka, Hideaki; Saito, Mikako; Matsuoka, Ken.

In: Plant and Cell Physiology, Vol. 48, No. 10, 10.2007, p. 1404-1413.

Research output: Contribution to journal › Article › peer-review

Shinya, T , Gális, I, Narisawa, T, Sasaki, M, Fukuda, H, Matsuoka, H, Saito, M & Matsuoka, K 2007, 'Comprehensive analysis of glucan elicitor-regulated gene expression in tobacco BY-2 cells reveals a novel MYB transcription factor involved in the regulation of phenylpropanoid metabolism', Plant and Cell Physiology, vol. 48, no. 10, pp. 1404-1413. https://doi.org/10.1093/pcp/pcm115

Shinya, Tomonori ; Gális, Ivan ; Narisawa, Tomoko ; Sasaki, Mami ; Fukuda, Hiroo ; Matsuoka, Hideaki ; Saito, Mikako ; Matsuoka, Ken. / Comprehensive analysis of glucan elicitor-regulated gene expression in tobacco BY-2 cells reveals a novel MYB transcription factor involved in the regulation of phenylpropanoid metabolism. In: Plant and Cell Physiology. 2007 ; Vol. 48, No. 10. pp. 1404-1413.

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title = "Comprehensive analysis of glucan elicitor-regulated gene expression in tobacco BY-2 cells reveals a novel MYB transcription factor involved in the regulation of phenylpropanoid metabolism",

abstract = "We previously demonstrated that a β-1,3-, 1,6-oligoglucan (AaGlucan) from the fungus Alternaria alternata 102 shows strong elicitor activity in tobacco BY-2 cells. We have used cDNA microarray analysis to monitor global changes in gene expression in tobacco cells treated with this A. alternata fraction or with laminarin. In total, we identified 265 genes that were induced 1 h after treatment with an AaGlucan-enriched fraction or laminarin. Among them, we characterized in detail a novel tobacco R2R3 MYB-type transcription factor homolog (NtMYBGR1) and two DC1 domain-containing genes (NtDC1A and NtDC1B). Microarray data, together with overexpression and metabolic analyses, indicated that NtMYBGR1, but not the NtDC1 proteins, primarily targets the phenylpropanoid synthesis-related genes PAL and 4CL. These results suggest that NtMYBGR1 specifically regulates defense responses in BY-2 cells by enhancing phenylpropanoid metabolism in response to AaGlucan and laminarin elicitors.",

keywords = "BY-2 cells, Gene expression, Glucan elicitor, MYB, Microarray, Phenylpropanoid pathway",

author = "Tomonori Shinya and Ivan G{\'a}lis and Tomoko Narisawa and Mami Sasaki and Hiroo Fukuda and Hideaki Matsuoka and Mikako Saito and Ken Matsuoka",

note = "Funding Information: This work was supported in part by a Grant-in-aid for Scientific Research of Priority Areas: Single-Cell Molecular Technology (No. 11227101), from the Ministry of Education, Culture, Sports, Science and Technology to H.M.",

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AU - Narisawa, Tomoko

AU - Sasaki, Mami

AU - Fukuda, Hiroo

AU - Matsuoka, Hideaki

AU - Saito, Mikako

AU - Matsuoka, Ken

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N2 - We previously demonstrated that a β-1,3-, 1,6-oligoglucan (AaGlucan) from the fungus Alternaria alternata 102 shows strong elicitor activity in tobacco BY-2 cells. We have used cDNA microarray analysis to monitor global changes in gene expression in tobacco cells treated with this A. alternata fraction or with laminarin. In total, we identified 265 genes that were induced 1 h after treatment with an AaGlucan-enriched fraction or laminarin. Among them, we characterized in detail a novel tobacco R2R3 MYB-type transcription factor homolog (NtMYBGR1) and two DC1 domain-containing genes (NtDC1A and NtDC1B). Microarray data, together with overexpression and metabolic analyses, indicated that NtMYBGR1, but not the NtDC1 proteins, primarily targets the phenylpropanoid synthesis-related genes PAL and 4CL. These results suggest that NtMYBGR1 specifically regulates defense responses in BY-2 cells by enhancing phenylpropanoid metabolism in response to AaGlucan and laminarin elicitors.

AB - We previously demonstrated that a β-1,3-, 1,6-oligoglucan (AaGlucan) from the fungus Alternaria alternata 102 shows strong elicitor activity in tobacco BY-2 cells. We have used cDNA microarray analysis to monitor global changes in gene expression in tobacco cells treated with this A. alternata fraction or with laminarin. In total, we identified 265 genes that were induced 1 h after treatment with an AaGlucan-enriched fraction or laminarin. Among them, we characterized in detail a novel tobacco R2R3 MYB-type transcription factor homolog (NtMYBGR1) and two DC1 domain-containing genes (NtDC1A and NtDC1B). Microarray data, together with overexpression and metabolic analyses, indicated that NtMYBGR1, but not the NtDC1 proteins, primarily targets the phenylpropanoid synthesis-related genes PAL and 4CL. These results suggest that NtMYBGR1 specifically regulates defense responses in BY-2 cells by enhancing phenylpropanoid metabolism in response to AaGlucan and laminarin elicitors.

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