Component analysis and growth process of nasopharyngeal calculus as revealed by Fourier transform infrared (FT-IR) spectroscopy

T Ogawa, A Shibata, Y Maeda, Y Uno, M Okano, K Nishizaki, K Ohsaki

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A quite rare case of nasopharyngeal calculus in a woman in her twenties associated with the nasal discharge of pseudomonas infection was reported. As the substance was irregularly large in size, we extracted it partially by piecemeal resection using forceps and also by cracking technique using the holmium yttrium-aluminum-garnet (YAG) laser, under saline irrigation and stereotactic microscopic navigator (SMN) system under endoscopic observation. The substance was firmly fixed to the pharyngeal tonsil bed. The final extract was a small piece of singly folded bandage, which is probably the focal background for calculus formation. In a cross section of calculus specimen removed during surgery, Fourier transform infrared (FT-IR) analysis revealed that a) signal ratio of methylene group (organic substance) to amide I (protein) was 21.6% at the nasal cavity side, gradually decreased toward nasal mucous membrane showing approximate 50%, b) signal ratio of amide I to P04(3-) (inorganic substance) ranged between 17.7% and 26.7% at the different sites and inside the calculus, the protein content was approximate 1/5 of the inorganic substance, and c) signal ratio of the methylene group to amide I at the nasal cavity site showed that their contents were almost equal. The quantity of the organic substance was estimated at approximate 1/2 quantity of the protein at both the central part and the part contacted with the mucous membrane. From these results, it seems that throughout the course of calculus growth, both inorganic substance and protein remain almost constant inside the calculus, while organic substance is released from the internal part of the calculus being probably formed at an early stage.
Original languageEnglish
Title of host publicationCell Mol Biol (Noisy-le-grand)
Pages621-626
Number of pages6
Publication statusPublished - 2003

Publication series

NameCell Mol Biol (Noisy-le-grand)
Volume49

Fingerprint

Calculi
Fourier Transform Infrared Spectroscopy
Growth
Amides
Nasal Cavity
Solid-State Lasers
Nose
Mucous Membrane
Proteins
Adenoids
Pseudomonas Infections
Fourier Analysis
Bandages
Surgical Instruments
Observation

Keywords

  • Adult
  • Calculi/*chemistry/metabolism
  • Female
  • Humans
  • Nasal Mucosa/*chemistry/metabolism
  • Nasopharyngeal Diseases/*metabolism
  • Pharynx/chemistry/metabolism
  • Spectroscopy, Fourier Transform Infrared
  • Turbinates/chemistry/metabolism

Cite this

Ogawa, T., Shibata, A., Maeda, Y., Uno, Y., Okano, M., Nishizaki, K., & Ohsaki, K. (2003). Component analysis and growth process of nasopharyngeal calculus as revealed by Fourier transform infrared (FT-IR) spectroscopy. In Cell Mol Biol (Noisy-le-grand) (pp. 621-626). (Cell Mol Biol (Noisy-le-grand); Vol. 49).

Component analysis and growth process of nasopharyngeal calculus as revealed by Fourier transform infrared (FT-IR) spectroscopy. / Ogawa, T; Shibata, A; Maeda, Y; Uno, Y; Okano, M; Nishizaki, K; Ohsaki, K.

Cell Mol Biol (Noisy-le-grand). 2003. p. 621-626 (Cell Mol Biol (Noisy-le-grand); Vol. 49).

Research output: Chapter in Book/Report/Conference proceedingChapter

Ogawa, T, Shibata, A, Maeda, Y, Uno, Y, Okano, M, Nishizaki, K & Ohsaki, K 2003, Component analysis and growth process of nasopharyngeal calculus as revealed by Fourier transform infrared (FT-IR) spectroscopy. in Cell Mol Biol (Noisy-le-grand). Cell Mol Biol (Noisy-le-grand), vol. 49, pp. 621-626.
Ogawa T, Shibata A, Maeda Y, Uno Y, Okano M, Nishizaki K et al. Component analysis and growth process of nasopharyngeal calculus as revealed by Fourier transform infrared (FT-IR) spectroscopy. In Cell Mol Biol (Noisy-le-grand). 2003. p. 621-626. (Cell Mol Biol (Noisy-le-grand)).
Ogawa, T ; Shibata, A ; Maeda, Y ; Uno, Y ; Okano, M ; Nishizaki, K ; Ohsaki, K. / Component analysis and growth process of nasopharyngeal calculus as revealed by Fourier transform infrared (FT-IR) spectroscopy. Cell Mol Biol (Noisy-le-grand). 2003. pp. 621-626 (Cell Mol Biol (Noisy-le-grand)).
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abstract = "A quite rare case of nasopharyngeal calculus in a woman in her twenties associated with the nasal discharge of pseudomonas infection was reported. As the substance was irregularly large in size, we extracted it partially by piecemeal resection using forceps and also by cracking technique using the holmium yttrium-aluminum-garnet (YAG) laser, under saline irrigation and stereotactic microscopic navigator (SMN) system under endoscopic observation. The substance was firmly fixed to the pharyngeal tonsil bed. The final extract was a small piece of singly folded bandage, which is probably the focal background for calculus formation. In a cross section of calculus specimen removed during surgery, Fourier transform infrared (FT-IR) analysis revealed that a) signal ratio of methylene group (organic substance) to amide I (protein) was 21.6{\%} at the nasal cavity side, gradually decreased toward nasal mucous membrane showing approximate 50{\%}, b) signal ratio of amide I to P04(3-) (inorganic substance) ranged between 17.7{\%} and 26.7{\%} at the different sites and inside the calculus, the protein content was approximate 1/5 of the inorganic substance, and c) signal ratio of the methylene group to amide I at the nasal cavity site showed that their contents were almost equal. The quantity of the organic substance was estimated at approximate 1/2 quantity of the protein at both the central part and the part contacted with the mucous membrane. From these results, it seems that throughout the course of calculus growth, both inorganic substance and protein remain almost constant inside the calculus, while organic substance is released from the internal part of the calculus being probably formed at an early stage.",
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AB - A quite rare case of nasopharyngeal calculus in a woman in her twenties associated with the nasal discharge of pseudomonas infection was reported. As the substance was irregularly large in size, we extracted it partially by piecemeal resection using forceps and also by cracking technique using the holmium yttrium-aluminum-garnet (YAG) laser, under saline irrigation and stereotactic microscopic navigator (SMN) system under endoscopic observation. The substance was firmly fixed to the pharyngeal tonsil bed. The final extract was a small piece of singly folded bandage, which is probably the focal background for calculus formation. In a cross section of calculus specimen removed during surgery, Fourier transform infrared (FT-IR) analysis revealed that a) signal ratio of methylene group (organic substance) to amide I (protein) was 21.6% at the nasal cavity side, gradually decreased toward nasal mucous membrane showing approximate 50%, b) signal ratio of amide I to P04(3-) (inorganic substance) ranged between 17.7% and 26.7% at the different sites and inside the calculus, the protein content was approximate 1/5 of the inorganic substance, and c) signal ratio of the methylene group to amide I at the nasal cavity site showed that their contents were almost equal. The quantity of the organic substance was estimated at approximate 1/2 quantity of the protein at both the central part and the part contacted with the mucous membrane. From these results, it seems that throughout the course of calculus growth, both inorganic substance and protein remain almost constant inside the calculus, while organic substance is released from the internal part of the calculus being probably formed at an early stage.

KW - Adult

KW - Calculi/chemistry/metabolism

KW - Female

KW - Humans

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KW - Nasopharyngeal Diseases/metabolism

KW - Pharynx/chemistry/metabolism

KW - Spectroscopy, Fourier Transform Infrared

KW - Turbinates/chemistry/metabolism

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