Complete sequence analysis of two cryptic plasmids from Bifidobacterium kashiwanohense JCM 15439 (type strain) isolated from healthy infant feces

Muneaki Takahata, Hidehiro Toh, Akiyo Nakano, Misako Takagi, Masaru Murakami, Yasuo Ishii, Tatsuya Takizawa, Soichi Tanabe, Hidetoshi Morita

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Bifidobacterial plasmids reported so far are derived from a limited number of strains and plasmids of bifidobacterial type strains isolated from humans are unknown. We found that Bifidobacterium kashiwanohenseJCM 15439 (type strain) isolated from a healthy infant contained two cryptic plasmids, designated pBBKW-1 and pBBKW-2. We determined and analyzed the complete sequences of both plasmids. pBBKW-1 (7716 bp) was predicted to replicate by a rolling-circle mechanism and encode six protein-coding genes, two of which are putative replication proteins. pBBKW-1 seems to be a cointegrate plasmid containing two copies of the plasmid pMG1 from Bifidobacterium longum. pBBKW-2 (2920 bp) was predicted to encode six protein-coding genes and be a theta-type replicating plasmid, which has been reported to be more stable than a rolling circle-type replicating plasmid frequently found in bifidobacteria. Our finding will provide new insights into safe recombinant plasmid constructions for humans.

Original languageEnglish
Pages (from-to)158-163
Number of pages6
JournalAnimal Science Journal
Volume85
Issue number2
DOIs
Publication statusPublished - Feb 1 2014
Externally publishedYes

Keywords

  • Bifidobacterium
  • Human origin
  • Plasmid

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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