Complete nucleotide sequence of a plasmid containing the botulinum neurotoxin gene in Clostridium botulinum type B strain 111 isolated from an infant patient in Japan

Koji Hosomi, Yoshihiko Sakaguchi, Tomoko Kohda, Kazuyoshi Goto, Daisuke Motooka, Shota Nakamura, Kaoru Umeda, Tetsuya Iida, Shunji Kozaki, Masafumi Mukamoto

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Botulinum neurotoxins (BoNTs) are highly potent toxins that are produced by Clostridiumbotulinum. We determined the complete nucleotide sequence of a plasmid containing the botulinum neurotoxin gene in C.botulinum type B strain 111 in order to obtain an insight into the toxigenicity and evolution of the bont gene in C.botulinum. Group I C.botulinum type B strain 111 was isolated from the first case of infant botulism in Japan in 1995. In previous studies, botulinum neurotoxin subtype B2 (BoNT/B2) produced by strain 111 exhibited different antigenic properties from those of authentic BoNT/B1 produced by strain Okra. We have recently shown that the isolates of strain 111 that lost toxigenicity were cured of the plasmid containing the bont/B2 gene. In the present study, the plasmid (named pCB111) was circular 265,575 bp double-stranded DNA and contained 332 predicted open reading frames (ORFs). 85 gene products of these ORFs could be functionally assigned on the basis of sequence homology to known proteins. The bont/B2 complex genes were located on pCB111 and some gene products may be involved in the conjugative plasmid transfer and horizontal transfer of bont genes. pCB111 was similar to previously identified plasmids containing bont/B1, /B5, or/A3 complex genes in other group I C.botulinum strains. It was suggested that these plasmids had been derived from a common ancestor and had played important roles for the bont gene transfer between C.botulinum.

Original languageEnglish
Pages (from-to)1267-1274
Number of pages8
JournalMolecular Genetics and Genomics
Volume289
Issue number6
DOIs
Publication statusPublished - Nov 19 2014
Externally publishedYes

Fingerprint

Clostridium botulinum type B
Neurotoxins
Japan
Plasmids
Genes
Open Reading Frames
Abelmoschus
Botulism
Horizontal Gene Transfer
Sequence Homology

Keywords

  • Infant botulism
  • Nucleotide sequence
  • Plasmid
  • Subtype B2 neurotoxin

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Medicine(all)

Cite this

Complete nucleotide sequence of a plasmid containing the botulinum neurotoxin gene in Clostridium botulinum type B strain 111 isolated from an infant patient in Japan. / Hosomi, Koji; Sakaguchi, Yoshihiko; Kohda, Tomoko; Goto, Kazuyoshi; Motooka, Daisuke; Nakamura, Shota; Umeda, Kaoru; Iida, Tetsuya; Kozaki, Shunji; Mukamoto, Masafumi.

In: Molecular Genetics and Genomics, Vol. 289, No. 6, 19.11.2014, p. 1267-1274.

Research output: Contribution to journalArticle

Hosomi, Koji ; Sakaguchi, Yoshihiko ; Kohda, Tomoko ; Goto, Kazuyoshi ; Motooka, Daisuke ; Nakamura, Shota ; Umeda, Kaoru ; Iida, Tetsuya ; Kozaki, Shunji ; Mukamoto, Masafumi. / Complete nucleotide sequence of a plasmid containing the botulinum neurotoxin gene in Clostridium botulinum type B strain 111 isolated from an infant patient in Japan. In: Molecular Genetics and Genomics. 2014 ; Vol. 289, No. 6. pp. 1267-1274.
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