Abstract
Botulinum neurotoxins (BoNTs) are highly potent toxins that are produced by Clostridiumbotulinum. We determined the complete nucleotide sequence of a plasmid containing the botulinum neurotoxin gene in C.botulinum type B strain 111 in order to obtain an insight into the toxigenicity and evolution of the bont gene in C.botulinum. Group I C.botulinum type B strain 111 was isolated from the first case of infant botulism in Japan in 1995. In previous studies, botulinum neurotoxin subtype B2 (BoNT/B2) produced by strain 111 exhibited different antigenic properties from those of authentic BoNT/B1 produced by strain Okra. We have recently shown that the isolates of strain 111 that lost toxigenicity were cured of the plasmid containing the bont/B2 gene. In the present study, the plasmid (named pCB111) was circular 265,575 bp double-stranded DNA and contained 332 predicted open reading frames (ORFs). 85 gene products of these ORFs could be functionally assigned on the basis of sequence homology to known proteins. The bont/B2 complex genes were located on pCB111 and some gene products may be involved in the conjugative plasmid transfer and horizontal transfer of bont genes. pCB111 was similar to previously identified plasmids containing bont/B1, /B5, or/A3 complex genes in other group I C.botulinum strains. It was suggested that these plasmids had been derived from a common ancestor and had played important roles for the bont gene transfer between C.botulinum.
| Original language | English |
|---|---|
| Pages (from-to) | 1267-1274 |
| Number of pages | 8 |
| Journal | Molecular Genetics and Genomics |
| Volume | 289 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Nov 19 2014 |
| Externally published | Yes |
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Keywords
- Infant botulism
- Nucleotide sequence
- Plasmid
- Subtype B2 neurotoxin
ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Medicine(all)
Cite this
Complete nucleotide sequence of a plasmid containing the botulinum neurotoxin gene in Clostridium botulinum type B strain 111 isolated from an infant patient in Japan. / Hosomi, Koji; Sakaguchi, Yoshihiko; Kohda, Tomoko; Goto, Kazuyoshi; Motooka, Daisuke; Nakamura, Shota; Umeda, Kaoru; Iida, Tetsuya; Kozaki, Shunji; Mukamoto, Masafumi.
In: Molecular Genetics and Genomics, Vol. 289, No. 6, 19.11.2014, p. 1267-1274.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Complete nucleotide sequence of a plasmid containing the botulinum neurotoxin gene in Clostridium botulinum type B strain 111 isolated from an infant patient in Japan
AU - Hosomi, Koji
AU - Sakaguchi, Yoshihiko
AU - Kohda, Tomoko
AU - Goto, Kazuyoshi
AU - Motooka, Daisuke
AU - Nakamura, Shota
AU - Umeda, Kaoru
AU - Iida, Tetsuya
AU - Kozaki, Shunji
AU - Mukamoto, Masafumi
PY - 2014/11/19
Y1 - 2014/11/19
N2 - Botulinum neurotoxins (BoNTs) are highly potent toxins that are produced by Clostridiumbotulinum. We determined the complete nucleotide sequence of a plasmid containing the botulinum neurotoxin gene in C.botulinum type B strain 111 in order to obtain an insight into the toxigenicity and evolution of the bont gene in C.botulinum. Group I C.botulinum type B strain 111 was isolated from the first case of infant botulism in Japan in 1995. In previous studies, botulinum neurotoxin subtype B2 (BoNT/B2) produced by strain 111 exhibited different antigenic properties from those of authentic BoNT/B1 produced by strain Okra. We have recently shown that the isolates of strain 111 that lost toxigenicity were cured of the plasmid containing the bont/B2 gene. In the present study, the plasmid (named pCB111) was circular 265,575 bp double-stranded DNA and contained 332 predicted open reading frames (ORFs). 85 gene products of these ORFs could be functionally assigned on the basis of sequence homology to known proteins. The bont/B2 complex genes were located on pCB111 and some gene products may be involved in the conjugative plasmid transfer and horizontal transfer of bont genes. pCB111 was similar to previously identified plasmids containing bont/B1, /B5, or/A3 complex genes in other group I C.botulinum strains. It was suggested that these plasmids had been derived from a common ancestor and had played important roles for the bont gene transfer between C.botulinum.
AB - Botulinum neurotoxins (BoNTs) are highly potent toxins that are produced by Clostridiumbotulinum. We determined the complete nucleotide sequence of a plasmid containing the botulinum neurotoxin gene in C.botulinum type B strain 111 in order to obtain an insight into the toxigenicity and evolution of the bont gene in C.botulinum. Group I C.botulinum type B strain 111 was isolated from the first case of infant botulism in Japan in 1995. In previous studies, botulinum neurotoxin subtype B2 (BoNT/B2) produced by strain 111 exhibited different antigenic properties from those of authentic BoNT/B1 produced by strain Okra. We have recently shown that the isolates of strain 111 that lost toxigenicity were cured of the plasmid containing the bont/B2 gene. In the present study, the plasmid (named pCB111) was circular 265,575 bp double-stranded DNA and contained 332 predicted open reading frames (ORFs). 85 gene products of these ORFs could be functionally assigned on the basis of sequence homology to known proteins. The bont/B2 complex genes were located on pCB111 and some gene products may be involved in the conjugative plasmid transfer and horizontal transfer of bont genes. pCB111 was similar to previously identified plasmids containing bont/B1, /B5, or/A3 complex genes in other group I C.botulinum strains. It was suggested that these plasmids had been derived from a common ancestor and had played important roles for the bont gene transfer between C.botulinum.
KW - Infant botulism
KW - Nucleotide sequence
KW - Plasmid
KW - Subtype B2 neurotoxin
UR - http://www.scopus.com/inward/record.url?scp=84919705481&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84919705481&partnerID=8YFLogxK
U2 - 10.1007/s00438-014-0887-4
DO - 10.1007/s00438-014-0887-4
M3 - Article
C2 - 25149145
AN - SCOPUS:84919705481
VL - 289
SP - 1267
EP - 1274
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
SN - 1617-4615
IS - 6
ER -