Complete nucleotide sequence and genome structure of a Japanese isolate of hibiscus latent Fort Pierce virus, a unique tobamovirus that contains an internal poly(A) region in its 3′ end

Tetsuya Yoshida, Yugo Kitazawa, Ken Komatsu, Yutaro Neriya, Kazuya Ishikawa, Naoko Fujita, Masayoshi Hashimoto, Kensaku Maejima, Yasuyuki Yamaji, Shigetou Namba

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

In this study, we detected a Japanese isolate of hibiscus latent Fort Pierce virus (HLFPV-J), a member of the genus Tobamovirus, in a hibiscus plant in Japan and determined the complete sequence and organization of its genome. HLFPV-J has four open reading frames (ORFs), each of which shares more than 98 % nucleotide sequence identity with those of other HLFPV isolates. Moreover, HLFPV-J contains a unique internal poly(A) region of variable length, ranging from 44 to 78 nucleotides, in its 3′-untranslated region (UTR), as is the case with hibiscus latent Singapore virus (HLSV), another hibiscus-infecting tobamovirus. The length of the HLFPV-J genome was 6431 nucleotides, including the shortest internal poly(A) region. The sequence identities of ORFs 1, 2, 3 and 4 of HLFPV-J to other tobamoviruses were 46.6–68.7, 49.9–70.8, 31.0–70.8 and 39.4–70.1 %, respectively, at the nucleotide level and 39.8–75.0, 43.6–77.8, 19.2–70.4 and 31.2–74.2 %, respectively, at the amino acid level. The 5′- and 3′-UTRs of HLFPV-J showed 24.3–58.6 and 13.0–79.8 % identity, respectively, to other tobamoviruses. In particular, when compared to other tobamoviruses, each ORF and UTR of HLFPV-J showed the highest sequence identity to those of HLSV. Phylogenetic analysis showed that HLFPV-J, other HLFPV isolates and HLSV constitute a malvaceous-plant-infecting tobamovirus cluster. These results indicate that the genomic structure of HLFPV-J has unique features similar to those of HLSV. To our knowledge, this is the first report of the complete genome sequence of HLFPV.

Original languageEnglish
Pages (from-to)3161-3165
Number of pages5
JournalArchives of Virology
Volume159
Issue number11
DOIs
Publication statusPublished - Oct 17 2014
Externally publishedYes

ASJC Scopus subject areas

  • Virology

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