Complete mitochondrial genome sequence of black mustard (Brassica nigra; BB) and comparison with Brassica oleracea (CC) and Brassica carinata (BBCC)

Hiroshi Yamagishi, Yoshiyuki Tanaka, Toru Terachi, L. Bonen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Crop species of Brassica (Brassicaceae) consist of three monogenomic species and three amphidiploid species resulting from interspecific hybridizations among them. Until now, mitochondrial genome sequences were available for only five of these species. We sequenced the mitochondrial genome of the sixth species, Brassica nigra (nuclear genome constitution BB), and compared it with those of Brassica oleracea (CC) and Brassica carinata (BBCC). The genome was assembled into a 232 145 bp circular sequence that is slightly larger than that of B. oleracea (219 952 bp). The genome of B. nigra contained 33 protein-coding genes, 3 rRNA genes, and 17 tRNA genes. The cox2-2 gene present in B. oleracea was absent in B. nigra. Although the nucleotide sequences of 52 genes were identical between B. nigra and B. carinata, the second exon of rps3 showed differences including an insertion/deletion (indel) and nucleotide substitutions. A PCR test to detect the indel revealed intraspecific variation in rps3, and in one line of B. nigra it amplified a DNA fragment of the size expected for B. carinata. In addition, the B. carinata lines tested here produced DNA fragments of the size expected for B. nigra. The results indicate that at least two mitotypes of B. nigra were present in the maternal parents of B. carinata.

Original languageEnglish
Pages (from-to)577-582
Number of pages6
JournalGenome
Volume57
Issue number11-12
DOIs
Publication statusPublished - Feb 9 2015

Fingerprint

Mustard Plant
Mitochondrial Genome
Brassica
Genome
Genes
Brassicaceae
DNA
Constitution and Bylaws
Transfer RNA
rRNA Genes
Exons
Nucleotides
Mothers
Polymerase Chain Reaction

Keywords

  • Brassica carinata
  • Brassica nigra
  • Complete mitochondrial genome
  • Intraspecific mitotype variation
  • Maternal parent of alloploid

ASJC Scopus subject areas

  • Biotechnology
  • Genetics
  • Molecular Biology
  • Medicine(all)

Cite this

Complete mitochondrial genome sequence of black mustard (Brassica nigra; BB) and comparison with Brassica oleracea (CC) and Brassica carinata (BBCC). / Yamagishi, Hiroshi; Tanaka, Yoshiyuki; Terachi, Toru; Bonen, L.

In: Genome, Vol. 57, No. 11-12, 09.02.2015, p. 577-582.

Research output: Contribution to journalArticle

Yamagishi, Hiroshi ; Tanaka, Yoshiyuki ; Terachi, Toru ; Bonen, L. / Complete mitochondrial genome sequence of black mustard (Brassica nigra; BB) and comparison with Brassica oleracea (CC) and Brassica carinata (BBCC). In: Genome. 2015 ; Vol. 57, No. 11-12. pp. 577-582.
@article{93117785fbe14844bce6fe36ea9f0bf4,
title = "Complete mitochondrial genome sequence of black mustard (Brassica nigra; BB) and comparison with Brassica oleracea (CC) and Brassica carinata (BBCC)",
abstract = "Crop species of Brassica (Brassicaceae) consist of three monogenomic species and three amphidiploid species resulting from interspecific hybridizations among them. Until now, mitochondrial genome sequences were available for only five of these species. We sequenced the mitochondrial genome of the sixth species, Brassica nigra (nuclear genome constitution BB), and compared it with those of Brassica oleracea (CC) and Brassica carinata (BBCC). The genome was assembled into a 232 145 bp circular sequence that is slightly larger than that of B. oleracea (219 952 bp). The genome of B. nigra contained 33 protein-coding genes, 3 rRNA genes, and 17 tRNA genes. The cox2-2 gene present in B. oleracea was absent in B. nigra. Although the nucleotide sequences of 52 genes were identical between B. nigra and B. carinata, the second exon of rps3 showed differences including an insertion/deletion (indel) and nucleotide substitutions. A PCR test to detect the indel revealed intraspecific variation in rps3, and in one line of B. nigra it amplified a DNA fragment of the size expected for B. carinata. In addition, the B. carinata lines tested here produced DNA fragments of the size expected for B. nigra. The results indicate that at least two mitotypes of B. nigra were present in the maternal parents of B. carinata.",
keywords = "Brassica carinata, Brassica nigra, Complete mitochondrial genome, Intraspecific mitotype variation, Maternal parent of alloploid",
author = "Hiroshi Yamagishi and Yoshiyuki Tanaka and Toru Terachi and L. Bonen",
year = "2015",
month = "2",
day = "9",
doi = "10.1139/gen-2014-0165",
language = "English",
volume = "57",
pages = "577--582",
journal = "Genome / National Research Council Canada = Genome / Conseil national de recherches Canada",
issn = "0831-2796",
publisher = "National Research Council of Canada",
number = "11-12",

}

TY - JOUR

T1 - Complete mitochondrial genome sequence of black mustard (Brassica nigra; BB) and comparison with Brassica oleracea (CC) and Brassica carinata (BBCC)

AU - Yamagishi, Hiroshi

AU - Tanaka, Yoshiyuki

AU - Terachi, Toru

AU - Bonen, L.

PY - 2015/2/9

Y1 - 2015/2/9

N2 - Crop species of Brassica (Brassicaceae) consist of three monogenomic species and three amphidiploid species resulting from interspecific hybridizations among them. Until now, mitochondrial genome sequences were available for only five of these species. We sequenced the mitochondrial genome of the sixth species, Brassica nigra (nuclear genome constitution BB), and compared it with those of Brassica oleracea (CC) and Brassica carinata (BBCC). The genome was assembled into a 232 145 bp circular sequence that is slightly larger than that of B. oleracea (219 952 bp). The genome of B. nigra contained 33 protein-coding genes, 3 rRNA genes, and 17 tRNA genes. The cox2-2 gene present in B. oleracea was absent in B. nigra. Although the nucleotide sequences of 52 genes were identical between B. nigra and B. carinata, the second exon of rps3 showed differences including an insertion/deletion (indel) and nucleotide substitutions. A PCR test to detect the indel revealed intraspecific variation in rps3, and in one line of B. nigra it amplified a DNA fragment of the size expected for B. carinata. In addition, the B. carinata lines tested here produced DNA fragments of the size expected for B. nigra. The results indicate that at least two mitotypes of B. nigra were present in the maternal parents of B. carinata.

AB - Crop species of Brassica (Brassicaceae) consist of three monogenomic species and three amphidiploid species resulting from interspecific hybridizations among them. Until now, mitochondrial genome sequences were available for only five of these species. We sequenced the mitochondrial genome of the sixth species, Brassica nigra (nuclear genome constitution BB), and compared it with those of Brassica oleracea (CC) and Brassica carinata (BBCC). The genome was assembled into a 232 145 bp circular sequence that is slightly larger than that of B. oleracea (219 952 bp). The genome of B. nigra contained 33 protein-coding genes, 3 rRNA genes, and 17 tRNA genes. The cox2-2 gene present in B. oleracea was absent in B. nigra. Although the nucleotide sequences of 52 genes were identical between B. nigra and B. carinata, the second exon of rps3 showed differences including an insertion/deletion (indel) and nucleotide substitutions. A PCR test to detect the indel revealed intraspecific variation in rps3, and in one line of B. nigra it amplified a DNA fragment of the size expected for B. carinata. In addition, the B. carinata lines tested here produced DNA fragments of the size expected for B. nigra. The results indicate that at least two mitotypes of B. nigra were present in the maternal parents of B. carinata.

KW - Brassica carinata

KW - Brassica nigra

KW - Complete mitochondrial genome

KW - Intraspecific mitotype variation

KW - Maternal parent of alloploid

UR - http://www.scopus.com/inward/record.url?scp=84927740782&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84927740782&partnerID=8YFLogxK

U2 - 10.1139/gen-2014-0165

DO - 10.1139/gen-2014-0165

M3 - Article

C2 - 25767903

AN - SCOPUS:84927740782

VL - 57

SP - 577

EP - 582

JO - Genome / National Research Council Canada = Genome / Conseil national de recherches Canada

JF - Genome / National Research Council Canada = Genome / Conseil national de recherches Canada

SN - 0831-2796

IS - 11-12

ER -