TY - JOUR
T1 - Complete genome sequence of a Japanese isolate of Daphne virus S
AU - Fujita, Naoko
AU - Komatsu, Ken
AU - Neriya, Yutaro
AU - Kagiwada, Satoshi
AU - Hara, Shinichiro
AU - Miyazaki, Akio
AU - Netsu, Osamu
AU - Hashimoto, Masayoshi
AU - Yamaji, Yasuyuki
AU - Namba, Shigetou
N1 - Publisher Copyright:
© 2015 The Japan Mendel Society.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - Daphne virus S (DVS), a member of the genus Carlavirus, was isolated from a daphne plant in Japan. The genome of the isolate (DVS-JP) consisted of 8736 nucleotides and was predicted to contain six open reading frames (ORFs). The overall genome sequence identity between DVS-JP and the previously reported Korean isolate of DVS (DVS-K) was 83.0%, whereas identities to other carlaviruses ranged from 20.5-56.0%. Each DVS-JP ORF shared more than 80% identity at both the nucleotide and amino acid levels with DVS-K, but less than 56% with other carlaviruses, which confirmed that DVS-JP was the same species as DVS-K. The RNA dependent RNA polymerase (RdRp) showed the highest identity with Phlox virus S, and the coat protein (CP) showed the highest identity with Helenium virus S and Chrysanthemum virus B. The genome sequence of an isolate of DVS-JP revealed a high degree of identity with DVS-K, which increases our understanding of the evolution of the genus Carlavirus.
AB - Daphne virus S (DVS), a member of the genus Carlavirus, was isolated from a daphne plant in Japan. The genome of the isolate (DVS-JP) consisted of 8736 nucleotides and was predicted to contain six open reading frames (ORFs). The overall genome sequence identity between DVS-JP and the previously reported Korean isolate of DVS (DVS-K) was 83.0%, whereas identities to other carlaviruses ranged from 20.5-56.0%. Each DVS-JP ORF shared more than 80% identity at both the nucleotide and amino acid levels with DVS-K, but less than 56% with other carlaviruses, which confirmed that DVS-JP was the same species as DVS-K. The RNA dependent RNA polymerase (RdRp) showed the highest identity with Phlox virus S, and the coat protein (CP) showed the highest identity with Helenium virus S and Chrysanthemum virus B. The genome sequence of an isolate of DVS-JP revealed a high degree of identity with DVS-K, which increases our understanding of the evolution of the genus Carlavirus.
KW - Carlavirus
KW - Complete genome
KW - Daphne virus S
KW - Japan
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U2 - 10.1508/cytologia.80.327
DO - 10.1508/cytologia.80.327
M3 - Article
AN - SCOPUS:84943191078
SN - 0011-4545
VL - 80
SP - 327
EP - 330
JO - Cytologia
JF - Cytologia
IS - 3
ER -