Complete amino acid sequence of a human monocyte chemoattractant, a putative mediator of cellular immune reactions

E. A. Robinson, T. Yoshimura, E. J. Leonard, S. Tanaka, P. R. Griffin, J. Shabanowitz, D. F. Hunt, E. Appella

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

In a study of the structural basis for leukocyte specificity of chemoattractants, we determined the complete amino acid sequence of human glioma-derived monocyte chemotactic factor (GDCF-2), a peptide that attracts human monocytes but not neutrophils. The choice of a tumor cell product for analysis was dictated by its relative abundance and an amino composition indistinguishable from that of lymphocyte-derived chemotactic factor (LDCF), the agonist thought to account for monocyte accumulation in cellular immune reactions. By a combination of Edman degradation and mass spectrometry, it was established that GDCF-2 comprises 76 amino acid residues, commencing at the N terminus with pyroglutamic acid. The peptide contains four half-cystines, at positions 11, 12, 36, and 52, which create a pair of loops, clustered at the disulfide bridges. The relative positions of the half-cystines are almost identical to those of monocyte-derived neutrophil chemotactic factor (MDNCF), a peptide of similar mass but with only 24% sequence identity to GDCF. Thus, GDCF and MDNCF have a similar gross secondary structure because of the loops formed by the clustered disulfides, and their different leukocyte specificities are most likely determined by the large differences in primary sequence.

Original languageEnglish
Pages (from-to)1850-1854
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number6
DOIs
Publication statusPublished - Jan 1 1989
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Complete amino acid sequence of a human monocyte chemoattractant, a putative mediator of cellular immune reactions'. Together they form a unique fingerprint.

  • Cite this