Compartmentation of aluminium in leaves of an Al-accumulator, Fagopyrum esculentum Moench.

Renfang Shen, Jian Feng Ma, Masaharu Kyo, Takashi Iwashita

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Buckwheat (Fagopyrum esculentum Moench.) is an Al-accumulating plant, but the internal mechanism(s) of detoxification of Al is not fully understood. We investigated the subcellular localization of Al in the leaves of this plant (cv. Jianxi) by directly isolating protoplasts and vacuoles. Pure protoplasts and vacuoles from the leaves of buckwheat, grown hydroponically in Al solution, were obtained based on light-microscopic observation and the activities of marker enzymes of cytosol and vacuoles. More than 80% of total Al in the leaves was present in the protoplasts, and was identified as an Al-oxalate complex (1:3 ratio) by 27A1-nuclear magnetic resonance. Oxalate and Al in the protoplasts was localized in the vacuoles. These results suggest that internal detoxification of Al in the buckwheat leaves is achieved by both complexation with oxalate and sequestration into vacuoles.

Original languageEnglish
Pages (from-to)394-398
Number of pages5
JournalPlanta
Volume215
Issue number3
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Fagopyrum
Fagopyrum esculentum
Vacuoles
Aluminum
Protoplasts
vacuoles
aluminum
protoplasts
Oxalates
buckwheat
oxalates
leaves
Plant Leaves
cytosol
Cytosol
nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Light
Enzymes
enzymes

Keywords

  • Aluminium
  • Compartmentation (A1)
  • Fagopyrum (A1 compartmentation)
  • Protoplast
  • Vacuole

ASJC Scopus subject areas

  • Plant Science

Cite this

Compartmentation of aluminium in leaves of an Al-accumulator, Fagopyrum esculentum Moench. / Shen, Renfang; Ma, Jian Feng; Kyo, Masaharu; Iwashita, Takashi.

In: Planta, Vol. 215, No. 3, 2002, p. 394-398.

Research output: Contribution to journalArticle

Shen, Renfang ; Ma, Jian Feng ; Kyo, Masaharu ; Iwashita, Takashi. / Compartmentation of aluminium in leaves of an Al-accumulator, Fagopyrum esculentum Moench. In: Planta. 2002 ; Vol. 215, No. 3. pp. 394-398.
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