Abstract
For successful sterile insect technique (SIT), synchronized copulation between invaded females and sterilized males is required. Understanding the mating time of the invaded strain is an aid in synchronizing and thus improving the effectiveness of SIT. We previously demonstrated a relationship between variation at two sites of a circadian clock gene cryptochrome (cry) (cry1212 and cry1865) and circadian behavior in the melon fly Bactrocera cucurbitae (Coquillett). Here we investigated the relationship in two other populations, Taiwan1 (T1) and Taiwan2 (T2), which may re-invade Okinawa. The results showed that T1 exhibited a lower frequency of the S-type allele, which was observed in early mating flies in the strains in Okinawa, than T2 at the site of cry1212. In addition, T1 showed a longer circadian period than T2. We also noted that the cry1212 site showed higher amino acid sequence conservation than cry1865 by comparing CRY1 among five insect species. These results suggest that genotyping of only the cry1212 site of trapped flies enables an immediate estimate of the mating time of the B. cucurbitae population from Taiwan and that cry1212 would be more likely to be involved in determining the mating time than cry1865.
| Original language | English |
|---|---|
| Pages (from-to) | 553-557 |
| Number of pages | 5 |
| Journal | Applied Entomology and Zoology |
| Volume | 46 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Nov 2011 |
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Keywords
- Bactrocera cucurbitae
- Clock gene
- Cryptochrome
- Sterile insect technique
- Tephritid flies
ASJC Scopus subject areas
- Insect Science
Cite this
Comparison of two polymorphic sites in the clock gene cryptochrome in the Taiwan strain of the melon fly, Bactrocera cucurbitae (Diptera : Tephritidae): A possible quick method to estimate the mating time of trapped invading flies. / Fuchikawa, Taro; Matsuyama, Takashi; Yamagishi, Masaaki; Nakayama, Satoshi; Okada, Kensuke; Miyatake, Takahisa.
In: Applied Entomology and Zoology, Vol. 46, No. 4, 11.2011, p. 553-557.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of two polymorphic sites in the clock gene cryptochrome in the Taiwan strain of the melon fly, Bactrocera cucurbitae (Diptera
T2 - Tephritidae): A possible quick method to estimate the mating time of trapped invading flies
AU - Fuchikawa, Taro
AU - Matsuyama, Takashi
AU - Yamagishi, Masaaki
AU - Nakayama, Satoshi
AU - Okada, Kensuke
AU - Miyatake, Takahisa
PY - 2011/11
Y1 - 2011/11
N2 - For successful sterile insect technique (SIT), synchronized copulation between invaded females and sterilized males is required. Understanding the mating time of the invaded strain is an aid in synchronizing and thus improving the effectiveness of SIT. We previously demonstrated a relationship between variation at two sites of a circadian clock gene cryptochrome (cry) (cry1212 and cry1865) and circadian behavior in the melon fly Bactrocera cucurbitae (Coquillett). Here we investigated the relationship in two other populations, Taiwan1 (T1) and Taiwan2 (T2), which may re-invade Okinawa. The results showed that T1 exhibited a lower frequency of the S-type allele, which was observed in early mating flies in the strains in Okinawa, than T2 at the site of cry1212. In addition, T1 showed a longer circadian period than T2. We also noted that the cry1212 site showed higher amino acid sequence conservation than cry1865 by comparing CRY1 among five insect species. These results suggest that genotyping of only the cry1212 site of trapped flies enables an immediate estimate of the mating time of the B. cucurbitae population from Taiwan and that cry1212 would be more likely to be involved in determining the mating time than cry1865.
AB - For successful sterile insect technique (SIT), synchronized copulation between invaded females and sterilized males is required. Understanding the mating time of the invaded strain is an aid in synchronizing and thus improving the effectiveness of SIT. We previously demonstrated a relationship between variation at two sites of a circadian clock gene cryptochrome (cry) (cry1212 and cry1865) and circadian behavior in the melon fly Bactrocera cucurbitae (Coquillett). Here we investigated the relationship in two other populations, Taiwan1 (T1) and Taiwan2 (T2), which may re-invade Okinawa. The results showed that T1 exhibited a lower frequency of the S-type allele, which was observed in early mating flies in the strains in Okinawa, than T2 at the site of cry1212. In addition, T1 showed a longer circadian period than T2. We also noted that the cry1212 site showed higher amino acid sequence conservation than cry1865 by comparing CRY1 among five insect species. These results suggest that genotyping of only the cry1212 site of trapped flies enables an immediate estimate of the mating time of the B. cucurbitae population from Taiwan and that cry1212 would be more likely to be involved in determining the mating time than cry1865.
KW - Bactrocera cucurbitae
KW - Clock gene
KW - Cryptochrome
KW - Sterile insect technique
KW - Tephritid flies
UR - http://www.scopus.com/inward/record.url?scp=80155208290&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80155208290&partnerID=8YFLogxK
U2 - 10.1007/s13355-011-0075-5
DO - 10.1007/s13355-011-0075-5
M3 - Article
VL - 46
SP - 553
EP - 557
JO - Applied Entomology and Zoology
JF - Applied Entomology and Zoology
SN - 0003-6862
IS - 4
ER -