Comparison of the temperature influence on the activity of currently available Procalcitonin reagents

Nori Yoshioka, Matsuo Deguchi, Hideharu Hagiya, Masanori Kagita, Satomi Yukawa, Yoh Hidaka, Kazunori Tomono

Research output: Contribution to journalArticle

Abstract

Background: Procalcitonin (PCT) is a stable biomarker for bacterial infections; however, limited data is available on new trivalent reagents. We evaluated temperature influence on the activity of PCT reagents. Methods: Using both conventional and trivalent reagents, we measured PCT levels of 30 clinical samples, stored residuum at refrigerator (4°C) and room temperature (24°C), and reexamined it after 24 hours. We defined a reduction rate as a percentage of PCT level at 24 hours compared to that after defrost and evaluated a ratio of reduction rate in 4°C to that in 24°C. Results: The reduction rate at room temperature decreased significantly compared to that in the refrigerated condition for all the reagents examined (p < 0.001). In addition, the ratio of reduction rate between the conventional and trivalent reagents showed a significant difference (p < 0.001) Conclusions: The serum PCT levels significantly decrease at room temperature, particularly when using newer trivalent reagents.

Original languageEnglish
Pages (from-to)201-204
Number of pages4
JournalClinical Laboratory
Volume64
Issue number1-2
DOIs
Publication statusPublished - Jan 1 2018
Externally publishedYes

Keywords

  • Anti-n-procalcitonin antibody
  • Bacterial infection
  • Biomarker
  • Refrigeration
  • Room temperature

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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