Comparison of the Cariostatic Effects between Regimens to Administer Oolong Tea Polyphenols in SPF Rats

T. Ooshima, T. Minami, Michiyo Nakano, T. Fujiwara, S. Sobue, S. Hamada

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The cariostatic effect of oolong tea polyphenols administered according to several regimens was examined in specific pathogen-free (SPF) Sprague-Dawley rats given both a diet containing 20% sucrose and infected with S. sobrinus 6715. The crude preparation (OTE) of oolong tea polyphenols showed the most prominent effect on caries reduction in SPF rats when OTE was administered in the drinking water beginning 1 day prior to the inoculation of S. sobrinus 6715, when compared with chromatographically isolated polyphenol fractions (OTF1 and OTF6) of OTE. Reduction in caries development was found even when OTE was given 1 day after inoculation of the organism. OTE was shown to significantly inhibit dental caries in rats at the concentrations of either more than 5 μg/ml in drinking water or more than 10 μg/g in diet. OTF1 and OTF6 also showed significant inhibition of caries induction, with the minimum inhibitory concentration of OTF6 being 50 μg/ml in drinking water and the minimum inhibitory concentration of OTF1 being 100 μg/g in diet. These results indicate that cariostatic activity of OTE was effective even after the establishment of S. sobrinus in the oral cavity and was more effective in drinking water than in diet. Furthermore, OTE may contain some anticaries substances that affect the virulence of S. sobrinus other than glucosyltransferases.

Original languageEnglish
Pages (from-to)75-80
Number of pages6
JournalCaries Research
Volume32
Issue number1
Publication statusPublished - Jan 1998
Externally publishedYes

Fingerprint

Cariostatic Agents
Specific Pathogen-Free Organisms
Polyphenols
Tea
Drinking Water
Diet
Microbial Sensitivity Tests
Glucosyltransferases
Dental Caries
Sprague Dawley Rats
Virulence
Sucrose
Mouth

Keywords

  • Dental caries
  • Enzyme inhibitors
  • Mutans streptococci
  • Polyphenol
  • Tea

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Ooshima, T., Minami, T., Nakano, M., Fujiwara, T., Sobue, S., & Hamada, S. (1998). Comparison of the Cariostatic Effects between Regimens to Administer Oolong Tea Polyphenols in SPF Rats. Caries Research, 32(1), 75-80.

Comparison of the Cariostatic Effects between Regimens to Administer Oolong Tea Polyphenols in SPF Rats. / Ooshima, T.; Minami, T.; Nakano, Michiyo; Fujiwara, T.; Sobue, S.; Hamada, S.

In: Caries Research, Vol. 32, No. 1, 01.1998, p. 75-80.

Research output: Contribution to journalArticle

Ooshima, T, Minami, T, Nakano, M, Fujiwara, T, Sobue, S & Hamada, S 1998, 'Comparison of the Cariostatic Effects between Regimens to Administer Oolong Tea Polyphenols in SPF Rats', Caries Research, vol. 32, no. 1, pp. 75-80.
Ooshima, T. ; Minami, T. ; Nakano, Michiyo ; Fujiwara, T. ; Sobue, S. ; Hamada, S. / Comparison of the Cariostatic Effects between Regimens to Administer Oolong Tea Polyphenols in SPF Rats. In: Caries Research. 1998 ; Vol. 32, No. 1. pp. 75-80.
@article{052d9c2f0f4848cdb93e0e45aff3dbd4,
title = "Comparison of the Cariostatic Effects between Regimens to Administer Oolong Tea Polyphenols in SPF Rats",
abstract = "The cariostatic effect of oolong tea polyphenols administered according to several regimens was examined in specific pathogen-free (SPF) Sprague-Dawley rats given both a diet containing 20{\%} sucrose and infected with S. sobrinus 6715. The crude preparation (OTE) of oolong tea polyphenols showed the most prominent effect on caries reduction in SPF rats when OTE was administered in the drinking water beginning 1 day prior to the inoculation of S. sobrinus 6715, when compared with chromatographically isolated polyphenol fractions (OTF1 and OTF6) of OTE. Reduction in caries development was found even when OTE was given 1 day after inoculation of the organism. OTE was shown to significantly inhibit dental caries in rats at the concentrations of either more than 5 μg/ml in drinking water or more than 10 μg/g in diet. OTF1 and OTF6 also showed significant inhibition of caries induction, with the minimum inhibitory concentration of OTF6 being 50 μg/ml in drinking water and the minimum inhibitory concentration of OTF1 being 100 μg/g in diet. These results indicate that cariostatic activity of OTE was effective even after the establishment of S. sobrinus in the oral cavity and was more effective in drinking water than in diet. Furthermore, OTE may contain some anticaries substances that affect the virulence of S. sobrinus other than glucosyltransferases.",
keywords = "Dental caries, Enzyme inhibitors, Mutans streptococci, Polyphenol, Tea",
author = "T. Ooshima and T. Minami and Michiyo Nakano and T. Fujiwara and S. Sobue and S. Hamada",
year = "1998",
month = "1",
language = "English",
volume = "32",
pages = "75--80",
journal = "Caries Research",
issn = "0008-6568",
publisher = "S. Karger AG",
number = "1",

}

TY - JOUR

T1 - Comparison of the Cariostatic Effects between Regimens to Administer Oolong Tea Polyphenols in SPF Rats

AU - Ooshima, T.

AU - Minami, T.

AU - Nakano, Michiyo

AU - Fujiwara, T.

AU - Sobue, S.

AU - Hamada, S.

PY - 1998/1

Y1 - 1998/1

N2 - The cariostatic effect of oolong tea polyphenols administered according to several regimens was examined in specific pathogen-free (SPF) Sprague-Dawley rats given both a diet containing 20% sucrose and infected with S. sobrinus 6715. The crude preparation (OTE) of oolong tea polyphenols showed the most prominent effect on caries reduction in SPF rats when OTE was administered in the drinking water beginning 1 day prior to the inoculation of S. sobrinus 6715, when compared with chromatographically isolated polyphenol fractions (OTF1 and OTF6) of OTE. Reduction in caries development was found even when OTE was given 1 day after inoculation of the organism. OTE was shown to significantly inhibit dental caries in rats at the concentrations of either more than 5 μg/ml in drinking water or more than 10 μg/g in diet. OTF1 and OTF6 also showed significant inhibition of caries induction, with the minimum inhibitory concentration of OTF6 being 50 μg/ml in drinking water and the minimum inhibitory concentration of OTF1 being 100 μg/g in diet. These results indicate that cariostatic activity of OTE was effective even after the establishment of S. sobrinus in the oral cavity and was more effective in drinking water than in diet. Furthermore, OTE may contain some anticaries substances that affect the virulence of S. sobrinus other than glucosyltransferases.

AB - The cariostatic effect of oolong tea polyphenols administered according to several regimens was examined in specific pathogen-free (SPF) Sprague-Dawley rats given both a diet containing 20% sucrose and infected with S. sobrinus 6715. The crude preparation (OTE) of oolong tea polyphenols showed the most prominent effect on caries reduction in SPF rats when OTE was administered in the drinking water beginning 1 day prior to the inoculation of S. sobrinus 6715, when compared with chromatographically isolated polyphenol fractions (OTF1 and OTF6) of OTE. Reduction in caries development was found even when OTE was given 1 day after inoculation of the organism. OTE was shown to significantly inhibit dental caries in rats at the concentrations of either more than 5 μg/ml in drinking water or more than 10 μg/g in diet. OTF1 and OTF6 also showed significant inhibition of caries induction, with the minimum inhibitory concentration of OTF6 being 50 μg/ml in drinking water and the minimum inhibitory concentration of OTF1 being 100 μg/g in diet. These results indicate that cariostatic activity of OTE was effective even after the establishment of S. sobrinus in the oral cavity and was more effective in drinking water than in diet. Furthermore, OTE may contain some anticaries substances that affect the virulence of S. sobrinus other than glucosyltransferases.

KW - Dental caries

KW - Enzyme inhibitors

KW - Mutans streptococci

KW - Polyphenol

KW - Tea

UR - http://www.scopus.com/inward/record.url?scp=0031612445&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031612445&partnerID=8YFLogxK

M3 - Article

VL - 32

SP - 75

EP - 80

JO - Caries Research

JF - Caries Research

SN - 0008-6568

IS - 1

ER -