TY - JOUR
T1 - Comparison of the biological properties of purified natural and recombinant human interleukin-2
AU - Naruo, Ken ichi
AU - Hinuma, Shuji
AU - Kato, Koichi
AU - Koyama, Masaru
AU - Tada, Hiroko
AU - Shiho, Osamu
AU - Tsukamoto, Kyozo
PY - 1985/4/16
Y1 - 1985/4/16
N2 - We compared the biological properties of the purified recombinant human IL-2 derived from E. coli with those of purified natural IL-2. Both had almost the same specific in vitro activities on a weight basis to (i) support long-term proliferation of IL-2 dependent human peripheral blood lymphocytes, a mouse killer T cell line, and a mouse natural killer cell line; (ii) induce killer cells in normal mouse spleen cells; and (iii) induce antibody forming cells in nude mouse spleen cells. No differences in these biological activities were found between two forms of natural IL-2 that were separable by reverse phase high performance liquid chromatography.
AB - We compared the biological properties of the purified recombinant human IL-2 derived from E. coli with those of purified natural IL-2. Both had almost the same specific in vitro activities on a weight basis to (i) support long-term proliferation of IL-2 dependent human peripheral blood lymphocytes, a mouse killer T cell line, and a mouse natural killer cell line; (ii) induce killer cells in normal mouse spleen cells; and (iii) induce antibody forming cells in nude mouse spleen cells. No differences in these biological activities were found between two forms of natural IL-2 that were separable by reverse phase high performance liquid chromatography.
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U2 - 10.1016/0006-291X(85)91672-9
DO - 10.1016/0006-291X(85)91672-9
M3 - Article
C2 - 3872661
AN - SCOPUS:0022377663
SN - 0006-291X
VL - 128
SP - 257
EP - 264
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -