Comparison of the biological properties of purified natural and recombinant human interleukin-2

Ken ichi Naruo, Shuji Hinuma, Koichi Kato, Masaru Koyama, Hiroko Tada, Osamu Shiho, Kyozo Tsukamoto

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

We compared the biological properties of the purified recombinant human IL-2 derived from E. coli with those of purified natural IL-2. Both had almost the same specific in vitro activities on a weight basis to (i) support long-term proliferation of IL-2 dependent human peripheral blood lymphocytes, a mouse killer T cell line, and a mouse natural killer cell line; (ii) induce killer cells in normal mouse spleen cells; and (iii) induce antibody forming cells in nude mouse spleen cells. No differences in these biological activities were found between two forms of natural IL-2 that were separable by reverse phase high performance liquid chromatography.

Original languageEnglish
Pages (from-to)257-264
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume128
Issue number1
DOIs
Publication statusPublished - Apr 16 1985
Externally publishedYes

Fingerprint

Interleukin-2
Spleen
Cell Line
T-cells
Lymphocytes
High performance liquid chromatography
Reverse-Phase Chromatography
Bioactivity
Nude Mice
Natural Killer Cells
Escherichia coli
Blood
High Pressure Liquid Chromatography
Cells
T-Lymphocytes
Weights and Measures
Antibodies

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Comparison of the biological properties of purified natural and recombinant human interleukin-2. / Naruo, Ken ichi; Hinuma, Shuji; Kato, Koichi; Koyama, Masaru; Tada, Hiroko; Shiho, Osamu; Tsukamoto, Kyozo.

In: Biochemical and Biophysical Research Communications, Vol. 128, No. 1, 16.04.1985, p. 257-264.

Research output: Contribution to journalArticle

Naruo, Ken ichi ; Hinuma, Shuji ; Kato, Koichi ; Koyama, Masaru ; Tada, Hiroko ; Shiho, Osamu ; Tsukamoto, Kyozo. / Comparison of the biological properties of purified natural and recombinant human interleukin-2. In: Biochemical and Biophysical Research Communications. 1985 ; Vol. 128, No. 1. pp. 257-264.
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