Comparison of sequences of a transcriptional coactivator MBF2 from three lepidopteran species Bombyx mori, Bombyx mandarina and Samia cynthia

Qing Xin Liu; Hitoshi Ueda; Susumu Hirose

doi:10.1016/S0378-1119(98)00428-4

Comparison of sequences of a transcriptional coactivator MBF2 from three lepidopteran species Bombyx mori, Bombyx mandarina and Samia cynthia

Qing Xin Liu, Hitoshi Ueda, Susumu Hirose

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

MBF2 was first isolated from the silkworm Bombyx mori as a positive cofactor that activates transcription through its interaction with TFIIA. To identify conserved domain(s) within the MBF2 molecule, we isolated cDNAs encoding MBF2 homologues from other silkworms Bombyx mandarina and Samia cynthia. Bacterially expressed and purified MBF2 of B. mandarina and S. cynthia activated transcription in vitro. The predicted amino acid sequences of MBF2 from two Bombyx species share 97% homology. When we compared between B. mori and S. cynthia factors, the homology reduced to 50%. Four regions in MBF2 are conserved among these three species. Two of them are present in the middle region of MBF2 that is essential for the transcriptional activation.

Original language	English
Pages (from-to)	55-59
Number of pages	5
Journal	Gene
Volume	220
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-1119(98)00428-4
Publication status	Published - Oct 5 1998
Externally published	Yes

Keywords

FTZ-F1
MBF1
Silkworm
TFIIA

ASJC Scopus subject areas

Genetics

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10.1016/S0378-1119(98)00428-4

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@article{071820a8093048e7b7e819d08fc48ede,

title = "Comparison of sequences of a transcriptional coactivator MBF2 from three lepidopteran species Bombyx mori, Bombyx mandarina and Samia cynthia",

abstract = "MBF2 was first isolated from the silkworm Bombyx mori as a positive cofactor that activates transcription through its interaction with TFIIA. To identify conserved domain(s) within the MBF2 molecule, we isolated cDNAs encoding MBF2 homologues from other silkworms Bombyx mandarina and Samia cynthia. Bacterially expressed and purified MBF2 of B. mandarina and S. cynthia activated transcription in vitro. The predicted amino acid sequences of MBF2 from two Bombyx species share 97% homology. When we compared between B. mori and S. cynthia factors, the homology reduced to 50%. Four regions in MBF2 are conserved among these three species. Two of them are present in the middle region of MBF2 that is essential for the transcriptional activation.",

keywords = "FTZ-F1, MBF1, Silkworm, TFIIA",

author = "Liu, {Qing Xin} and Hitoshi Ueda and Susumu Hirose",

note = "Funding Information: We thank T. Tamura and K. Minato for a S. cynthia strain, and Y. Takada for preparation of the manuscript. This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan. Q.-X.L. was a Japanese Government Scholarship Student. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

year = "1998",

month = oct,

day = "5",

doi = "10.1016/S0378-1119(98)00428-4",

language = "English",

volume = "220",

pages = "55--59",

journal = "Gene",

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T1 - Comparison of sequences of a transcriptional coactivator MBF2 from three lepidopteran species Bombyx mori, Bombyx mandarina and Samia cynthia

AU - Liu, Qing Xin

AU - Ueda, Hitoshi

AU - Hirose, Susumu

N1 - Funding Information: We thank T. Tamura and K. Minato for a S. cynthia strain, and Y. Takada for preparation of the manuscript. This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan. Q.-X.L. was a Japanese Government Scholarship Student. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 1998/10/5

Y1 - 1998/10/5

N2 - MBF2 was first isolated from the silkworm Bombyx mori as a positive cofactor that activates transcription through its interaction with TFIIA. To identify conserved domain(s) within the MBF2 molecule, we isolated cDNAs encoding MBF2 homologues from other silkworms Bombyx mandarina and Samia cynthia. Bacterially expressed and purified MBF2 of B. mandarina and S. cynthia activated transcription in vitro. The predicted amino acid sequences of MBF2 from two Bombyx species share 97% homology. When we compared between B. mori and S. cynthia factors, the homology reduced to 50%. Four regions in MBF2 are conserved among these three species. Two of them are present in the middle region of MBF2 that is essential for the transcriptional activation.

AB - MBF2 was first isolated from the silkworm Bombyx mori as a positive cofactor that activates transcription through its interaction with TFIIA. To identify conserved domain(s) within the MBF2 molecule, we isolated cDNAs encoding MBF2 homologues from other silkworms Bombyx mandarina and Samia cynthia. Bacterially expressed and purified MBF2 of B. mandarina and S. cynthia activated transcription in vitro. The predicted amino acid sequences of MBF2 from two Bombyx species share 97% homology. When we compared between B. mori and S. cynthia factors, the homology reduced to 50%. Four regions in MBF2 are conserved among these three species. Two of them are present in the middle region of MBF2 that is essential for the transcriptional activation.

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