Abstract
MBF2 was first isolated from the silkworm Bombyx mori as a positive cofactor that activates transcription through its interaction with TFIIA. To identify conserved domain(s) within the MBF2 molecule, we isolated cDNAs encoding MBF2 homologues from other silkworms Bombyx mandarina and Samia cynthia. Bacterially expressed and purified MBF2 of B. mandarina and S. cynthia activated transcription in vitro. The predicted amino acid sequences of MBF2 from two Bombyx species share 97% homology. When we compared between B. mori and S. cynthia factors, the homology reduced to 50%. Four regions in MBF2 are conserved among these three species. Two of them are present in the middle region of MBF2 that is essential for the transcriptional activation.
| Original language | English |
|---|---|
| Pages (from-to) | 55-59 |
| Number of pages | 5 |
| Journal | Gene |
| Volume | 220 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Oct 5 1998 |
| Externally published | Yes |
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Keywords
- FTZ-F1
- MBF1
- Silkworm
- TFIIA
ASJC Scopus subject areas
- Genetics
Cite this
Comparison of sequences of a transcriptional coactivator MBF2 from three lepidopteran species Bombyx mori, Bombyx mandarina and Samia cynthia. / Liu, Qing Xin; Ueda, Hitoshi; Hirose, Susumu.
In: Gene, Vol. 220, No. 1-2, 05.10.1998, p. 55-59.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Comparison of sequences of a transcriptional coactivator MBF2 from three lepidopteran species Bombyx mori, Bombyx mandarina and Samia cynthia
AU - Liu, Qing Xin
AU - Ueda, Hitoshi
AU - Hirose, Susumu
PY - 1998/10/5
Y1 - 1998/10/5
N2 - MBF2 was first isolated from the silkworm Bombyx mori as a positive cofactor that activates transcription through its interaction with TFIIA. To identify conserved domain(s) within the MBF2 molecule, we isolated cDNAs encoding MBF2 homologues from other silkworms Bombyx mandarina and Samia cynthia. Bacterially expressed and purified MBF2 of B. mandarina and S. cynthia activated transcription in vitro. The predicted amino acid sequences of MBF2 from two Bombyx species share 97% homology. When we compared between B. mori and S. cynthia factors, the homology reduced to 50%. Four regions in MBF2 are conserved among these three species. Two of them are present in the middle region of MBF2 that is essential for the transcriptional activation.
AB - MBF2 was first isolated from the silkworm Bombyx mori as a positive cofactor that activates transcription through its interaction with TFIIA. To identify conserved domain(s) within the MBF2 molecule, we isolated cDNAs encoding MBF2 homologues from other silkworms Bombyx mandarina and Samia cynthia. Bacterially expressed and purified MBF2 of B. mandarina and S. cynthia activated transcription in vitro. The predicted amino acid sequences of MBF2 from two Bombyx species share 97% homology. When we compared between B. mori and S. cynthia factors, the homology reduced to 50%. Four regions in MBF2 are conserved among these three species. Two of them are present in the middle region of MBF2 that is essential for the transcriptional activation.
KW - FTZ-F1
KW - MBF1
KW - Silkworm
KW - TFIIA
UR - http://www.scopus.com/inward/record.url?scp=0032487410&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032487410&partnerID=8YFLogxK
U2 - 10.1016/S0378-1119(98)00428-4
DO - 10.1016/S0378-1119(98)00428-4
M3 - Article
C2 - 9767108
AN - SCOPUS:0032487410
VL - 220
SP - 55
EP - 59
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1-2
ER -