Comparison of inhibitory activity on calcium phosphate precipitation by acidic proline-rich proteins, statherin, and histatin-1

N. Tamaki, T. Tada, Manabu Morita, T. Watanabe

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

This study quantitatively compares the inhibition of calcium phosphate (CaP) precipitation by the salivary acidic proline-rich proteins (PRPs) statherin and histatin-1. Saliva and CaCl2 in 125 mM imidazole buffer (pH 7.0) were incubated with potassium phosphate and a hydroxyapatite (HAP) suspension, for 30 min at 25°C, then filtered through nitrocellulose. The calcium (Ca) concentration in the filtrate was measured by atomic absorption spectrophotometry, then deducted from that in the initial solution to determine the amount of CaP precipitation after 30 min. The values of the inhibitory activities on CaP precipitation relative to crude parotid saliva were 4.7, 4.9, 6.9, and 65.8 for histatin-1, large PRPs, small PRPs, and statherin, respectively.

Original languageEnglish
Pages (from-to)59-62
Number of pages4
JournalCalcified Tissue International
Volume71
Issue number1
DOIs
Publication statusPublished - Jul 1 2002
Externally publishedYes

Fingerprint

Histatins
Proline
Saliva
Salivary Proline-Rich Proteins
Atomic Spectrophotometry
Proteins
Collodion
Durapatite
Suspensions
Buffers
Calcium
calcium phosphate

Keywords

  • Calcium phosphate precipitation
  • Histatin-1
  • Inhibition
  • Proline-rich proteins
  • Statherin

ASJC Scopus subject areas

  • Endocrinology

Cite this

Comparison of inhibitory activity on calcium phosphate precipitation by acidic proline-rich proteins, statherin, and histatin-1. / Tamaki, N.; Tada, T.; Morita, Manabu; Watanabe, T.

In: Calcified Tissue International, Vol. 71, No. 1, 01.07.2002, p. 59-62.

Research output: Contribution to journalArticle

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