Abstract
This study quantitatively compares the inhibition of calcium phosphate (CaP) precipitation by the salivary acidic proline-rich proteins (PRPs) statherin and histatin-1. Saliva and CaCl2 in 125 mM imidazole buffer (pH 7.0) were incubated with potassium phosphate and a hydroxyapatite (HAP) suspension, for 30 min at 25°C, then filtered through nitrocellulose. The calcium (Ca) concentration in the filtrate was measured by atomic absorption spectrophotometry, then deducted from that in the initial solution to determine the amount of CaP precipitation after 30 min. The values of the inhibitory activities on CaP precipitation relative to crude parotid saliva were 4.7, 4.9, 6.9, and 65.8 for histatin-1, large PRPs, small PRPs, and statherin, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 59-62 |
| Number of pages | 4 |
| Journal | Calcified Tissue International |
| Volume | 71 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jul 1 2002 |
| Externally published | Yes |
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Keywords
- Calcium phosphate precipitation
- Histatin-1
- Inhibition
- Proline-rich proteins
- Statherin
ASJC Scopus subject areas
- Endocrinology
Cite this
Comparison of inhibitory activity on calcium phosphate precipitation by acidic proline-rich proteins, statherin, and histatin-1. / Tamaki, N.; Tada, T.; Morita, Manabu; Watanabe, T.
In: Calcified Tissue International, Vol. 71, No. 1, 01.07.2002, p. 59-62.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of inhibitory activity on calcium phosphate precipitation by acidic proline-rich proteins, statherin, and histatin-1
AU - Tamaki, N.
AU - Tada, T.
AU - Morita, Manabu
AU - Watanabe, T.
PY - 2002/7/1
Y1 - 2002/7/1
N2 - This study quantitatively compares the inhibition of calcium phosphate (CaP) precipitation by the salivary acidic proline-rich proteins (PRPs) statherin and histatin-1. Saliva and CaCl2 in 125 mM imidazole buffer (pH 7.0) were incubated with potassium phosphate and a hydroxyapatite (HAP) suspension, for 30 min at 25°C, then filtered through nitrocellulose. The calcium (Ca) concentration in the filtrate was measured by atomic absorption spectrophotometry, then deducted from that in the initial solution to determine the amount of CaP precipitation after 30 min. The values of the inhibitory activities on CaP precipitation relative to crude parotid saliva were 4.7, 4.9, 6.9, and 65.8 for histatin-1, large PRPs, small PRPs, and statherin, respectively.
AB - This study quantitatively compares the inhibition of calcium phosphate (CaP) precipitation by the salivary acidic proline-rich proteins (PRPs) statherin and histatin-1. Saliva and CaCl2 in 125 mM imidazole buffer (pH 7.0) were incubated with potassium phosphate and a hydroxyapatite (HAP) suspension, for 30 min at 25°C, then filtered through nitrocellulose. The calcium (Ca) concentration in the filtrate was measured by atomic absorption spectrophotometry, then deducted from that in the initial solution to determine the amount of CaP precipitation after 30 min. The values of the inhibitory activities on CaP precipitation relative to crude parotid saliva were 4.7, 4.9, 6.9, and 65.8 for histatin-1, large PRPs, small PRPs, and statherin, respectively.
KW - Calcium phosphate precipitation
KW - Histatin-1
KW - Inhibition
KW - Proline-rich proteins
KW - Statherin
UR - http://www.scopus.com/inward/record.url?scp=0036651781&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036651781&partnerID=8YFLogxK
U2 - 10.1007/s00223-001-1084-0
DO - 10.1007/s00223-001-1084-0
M3 - Article
C2 - 12060866
AN - SCOPUS:0036651781
VL - 71
SP - 59
EP - 62
JO - Calcified Tissue International
JF - Calcified Tissue International
SN - 0171-967X
IS - 1
ER -