Comparison of exocytotic mechanisms between acetylcholine- and catecholamine-containing vesicles in rat pheochromocytoma cells

Tei-ichi Nishiki, Yoko Shoji-Kasai, Mariko Sekiguchi, Satoshi Iwasaki, Konosuke Kumakura, Masami Takahashi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The molecular mechanisms of exocytosis from two types of secretory organelles, synaptic-like microvesicles and secretory vesicles, were compared by measuring acetylcholine (ACh) and catecholamine (CA) release from a newly isolated PC12 subclone, PC12-C3 which contains a high level of Ach. Digitonin-permeabilized PC12-C3 cells released both transmitters with similar Ca2+ dependency. Ca2+-evoked ACh and CA release from permeabilized cells were increased in the presence of MgATP, suggesting the existence of a MgATP-dependent priming step prior to the Ca2+-triggered fusion step in both ACh release and CA release. The non-hydrolyzable analogue of GTP, guanosine 5'(γ- thio)triphosphate (GTPγS), produced both ACh and CA release from permeabilized cells in the absence of Ca2+. Pretreatment with a phorbol ester which activates protein kinase C, potentiated depolarization-induced ACh and CA release from unpermeabilized cells. These results indicate that exocytosis from two distinct vesicle populations are mediated by the same basic molecular mechanisms.

Original languageEnglish
Pages (from-to)57-62
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume239
Issue number1
DOIs
Publication statusPublished - Oct 9 1997
Externally publishedYes

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Pheochromocytoma
Acetylcholine
Catecholamines
Rats
Exocytosis
Adenosine Triphosphate
Digitonin
Guanosine
PC12 Cells
Depolarization
Secretory Vesicles
Phorbol Esters
Guanosine Triphosphate
Organelles
Protein Kinase C
Transmitters
Fusion reactions
Population

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Comparison of exocytotic mechanisms between acetylcholine- and catecholamine-containing vesicles in rat pheochromocytoma cells. / Nishiki, Tei-ichi; Shoji-Kasai, Yoko; Sekiguchi, Mariko; Iwasaki, Satoshi; Kumakura, Konosuke; Takahashi, Masami.

In: Biochemical and Biophysical Research Communications, Vol. 239, No. 1, 09.10.1997, p. 57-62.

Research output: Contribution to journalArticle

Nishiki, Tei-ichi ; Shoji-Kasai, Yoko ; Sekiguchi, Mariko ; Iwasaki, Satoshi ; Kumakura, Konosuke ; Takahashi, Masami. / Comparison of exocytotic mechanisms between acetylcholine- and catecholamine-containing vesicles in rat pheochromocytoma cells. In: Biochemical and Biophysical Research Communications. 1997 ; Vol. 239, No. 1. pp. 57-62.
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