Comparison of efficacy of Salmonella typhimurium A1-R and chemotherapy on stem-like and non-stem human pancreatic cancer cells

Yukihiko Hiroshima; Ming Zhao; Yong Zhang; Ali Maawy; Mohamed K. Hassanein; Fuminari Uehara; Shinji Miwa; Shuya Yano; Masashi Momiyama; Atsushi Suetsugu; Takashi Chishima; Kuniya Tanaka; Michael Bouvet; Itaru Endo; Robert M. Hoffman

doi:10.4161/cc.25872

Comparison of efficacy of Salmonella typhimurium A1-R and chemotherapy on stem-like and non-stem human pancreatic cancer cells

Yukihiko Hiroshima, Ming Zhao, Yong Zhang, Ali Maawy, Mohamed K. Hassanein, Fuminari Uehara, Shinji Miwa, Shuya Yano, Masashi Momiyama, Atsushi Suetsugu, Takashi Chishima, Kuniya Tanaka, Michael Bouvet, Itaru Endo, Robert M. Hoffman

Research output: Contribution to journal › Article › peer-review

67 Citations (Scopus)

Abstract

The XPA1 human pancreatic cancer cell line is dimorphic, with spindle stem-like cells and round non-stem cells. We report here the in vitro IC50 values of stem-like and non-stem XPA1 human pancreatic cells cells for: (1) 5-fluorouracil (5-FU), (2) cisplatinum (CDDP), (3) gemcitabine (GEM), and (4) tumor-targeting Salmonella typhimurium A1-R (A1-R). IC50 values of stem-like XPA1 cells were significantly higher than those of non-stem XPA1 cells for 5-FU (P = 0.007) and CDDP (P = 0.012). In contrast, there was no difference between the efficacy of A1-R on stem-like and non-stem XPA1 cells. In vivo, 5-FU and A1-R significantly reduced the tumor weight of non-stem XPA1 cells (5-FU; P = 0.028; A1-R; P = 0.011). In contrast, only A1-R significantly reduced tumor weight of stem-like XPA1 cells (P = 0.012). The combination of A1-R with 5-FU improved the antitumor efficacy compared with 5-FU monotherapy on the stem-like cells (P = 0.004). The results of the present report indicate A1-R is a promising therapy for chemo-resistant pancreatic cancer stem-like cells.

Original language	English
Pages (from-to)	2774-2780
Number of pages	7
Journal	Cell Cycle
Volume	12
Issue number	17
DOIs	https://doi.org/10.4161/cc.25872
Publication status	Published - Sep 1 2013
Externally published	Yes

Keywords

Amino acid auxotrophy
Chemoresistance
Confocal microscopy
Fluorescence imaging
GFP
Pancreatic cancer
RFP
Salmonella typhimurium
Selective tumor targeting
Stem cell

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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Hiroshima, Y., Zhao, M., Zhang, Y., Maawy, A., Hassanein, M. K., Uehara, F., Miwa, S., Yano, S., Momiyama, M., Suetsugu, A., Chishima, T., Tanaka, K., Bouvet, M., Endo, I., & Hoffman, R. M. (2013). Comparison of efficacy of Salmonella typhimurium A1-R and chemotherapy on stem-like and non-stem human pancreatic cancer cells. Cell Cycle, 12(17), 2774-2780. https://doi.org/10.4161/cc.25872

Comparison of efficacy of Salmonella typhimurium A1-R and chemotherapy on stem-like and non-stem human pancreatic cancer cells. / Hiroshima, Yukihiko; Zhao, Ming; Zhang, Yong; Maawy, Ali; Hassanein, Mohamed K.; Uehara, Fuminari; Miwa, Shinji; Yano, Shuya; Momiyama, Masashi; Suetsugu, Atsushi; Chishima, Takashi; Tanaka, Kuniya; Bouvet, Michael; Endo, Itaru; Hoffman, Robert M.

In: Cell Cycle, Vol. 12, No. 17, 01.09.2013, p. 2774-2780.

Research output: Contribution to journal › Article › peer-review

Hiroshima, Y, Zhao, M, Zhang, Y, Maawy, A, Hassanein, MK, Uehara, F, Miwa, S, Yano, S, Momiyama, M, Suetsugu, A, Chishima, T, Tanaka, K, Bouvet, M, Endo, I & Hoffman, RM 2013, 'Comparison of efficacy of Salmonella typhimurium A1-R and chemotherapy on stem-like and non-stem human pancreatic cancer cells', Cell Cycle, vol. 12, no. 17, pp. 2774-2780. https://doi.org/10.4161/cc.25872

Hiroshima, Yukihiko ; Zhao, Ming ; Zhang, Yong ; Maawy, Ali ; Hassanein, Mohamed K. ; Uehara, Fuminari ; Miwa, Shinji ; Yano, Shuya ; Momiyama, Masashi ; Suetsugu, Atsushi ; Chishima, Takashi ; Tanaka, Kuniya ; Bouvet, Michael ; Endo, Itaru ; Hoffman, Robert M. / Comparison of efficacy of Salmonella typhimurium A1-R and chemotherapy on stem-like and non-stem human pancreatic cancer cells. In: Cell Cycle. 2013 ; Vol. 12, No. 17. pp. 2774-2780.

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title = "Comparison of efficacy of Salmonella typhimurium A1-R and chemotherapy on stem-like and non-stem human pancreatic cancer cells",

abstract = "The XPA1 human pancreatic cancer cell line is dimorphic, with spindle stem-like cells and round non-stem cells. We report here the in vitro IC50 values of stem-like and non-stem XPA1 human pancreatic cells cells for: (1) 5-fluorouracil (5-FU), (2) cisplatinum (CDDP), (3) gemcitabine (GEM), and (4) tumor-targeting Salmonella typhimurium A1-R (A1-R). IC50 values of stem-like XPA1 cells were significantly higher than those of non-stem XPA1 cells for 5-FU (P = 0.007) and CDDP (P = 0.012). In contrast, there was no difference between the efficacy of A1-R on stem-like and non-stem XPA1 cells. In vivo, 5-FU and A1-R significantly reduced the tumor weight of non-stem XPA1 cells (5-FU; P = 0.028; A1-R; P = 0.011). In contrast, only A1-R significantly reduced tumor weight of stem-like XPA1 cells (P = 0.012). The combination of A1-R with 5-FU improved the antitumor efficacy compared with 5-FU monotherapy on the stem-like cells (P = 0.004). The results of the present report indicate A1-R is a promising therapy for chemo-resistant pancreatic cancer stem-like cells.",

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author = "Yukihiko Hiroshima and Ming Zhao and Yong Zhang and Ali Maawy and Hassanein, {Mohamed K.} and Fuminari Uehara and Shinji Miwa and Shuya Yano and Masashi Momiyama and Atsushi Suetsugu and Takashi Chishima and Kuniya Tanaka and Michael Bouvet and Itaru Endo and Hoffman, {Robert M.}",

note = "Funding Information: This study was supported in part by National Cancer Institute grant CA126023 and Grants-in-Aid from the Japanese Ministry of Education, Culture, Sports, Science, and Technology for Fundamental Research (#23592018 to IE and # 24592009 to KT). This paper is dedicated to the memory of AR Moossa, MD.",

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T1 - Comparison of efficacy of Salmonella typhimurium A1-R and chemotherapy on stem-like and non-stem human pancreatic cancer cells

AU - Hiroshima, Yukihiko

AU - Zhao, Ming

AU - Zhang, Yong

AU - Maawy, Ali

AU - Hassanein, Mohamed K.

AU - Uehara, Fuminari

AU - Miwa, Shinji

AU - Yano, Shuya

AU - Momiyama, Masashi

AU - Suetsugu, Atsushi

AU - Chishima, Takashi

AU - Tanaka, Kuniya

AU - Bouvet, Michael

AU - Endo, Itaru

AU - Hoffman, Robert M.

N1 - Funding Information: This study was supported in part by National Cancer Institute grant CA126023 and Grants-in-Aid from the Japanese Ministry of Education, Culture, Sports, Science, and Technology for Fundamental Research (#23592018 to IE and # 24592009 to KT). This paper is dedicated to the memory of AR Moossa, MD.

PY - 2013/9/1

Y1 - 2013/9/1

N2 - The XPA1 human pancreatic cancer cell line is dimorphic, with spindle stem-like cells and round non-stem cells. We report here the in vitro IC50 values of stem-like and non-stem XPA1 human pancreatic cells cells for: (1) 5-fluorouracil (5-FU), (2) cisplatinum (CDDP), (3) gemcitabine (GEM), and (4) tumor-targeting Salmonella typhimurium A1-R (A1-R). IC50 values of stem-like XPA1 cells were significantly higher than those of non-stem XPA1 cells for 5-FU (P = 0.007) and CDDP (P = 0.012). In contrast, there was no difference between the efficacy of A1-R on stem-like and non-stem XPA1 cells. In vivo, 5-FU and A1-R significantly reduced the tumor weight of non-stem XPA1 cells (5-FU; P = 0.028; A1-R; P = 0.011). In contrast, only A1-R significantly reduced tumor weight of stem-like XPA1 cells (P = 0.012). The combination of A1-R with 5-FU improved the antitumor efficacy compared with 5-FU monotherapy on the stem-like cells (P = 0.004). The results of the present report indicate A1-R is a promising therapy for chemo-resistant pancreatic cancer stem-like cells.

AB - The XPA1 human pancreatic cancer cell line is dimorphic, with spindle stem-like cells and round non-stem cells. We report here the in vitro IC50 values of stem-like and non-stem XPA1 human pancreatic cells cells for: (1) 5-fluorouracil (5-FU), (2) cisplatinum (CDDP), (3) gemcitabine (GEM), and (4) tumor-targeting Salmonella typhimurium A1-R (A1-R). IC50 values of stem-like XPA1 cells were significantly higher than those of non-stem XPA1 cells for 5-FU (P = 0.007) and CDDP (P = 0.012). In contrast, there was no difference between the efficacy of A1-R on stem-like and non-stem XPA1 cells. In vivo, 5-FU and A1-R significantly reduced the tumor weight of non-stem XPA1 cells (5-FU; P = 0.028; A1-R; P = 0.011). In contrast, only A1-R significantly reduced tumor weight of stem-like XPA1 cells (P = 0.012). The combination of A1-R with 5-FU improved the antitumor efficacy compared with 5-FU monotherapy on the stem-like cells (P = 0.004). The results of the present report indicate A1-R is a promising therapy for chemo-resistant pancreatic cancer stem-like cells.

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KW - Selective tumor targeting

KW - Stem cell

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