Abstract
The XPA1 human pancreatic cancer cell line is dimorphic, with spindle stem-like cells and round non-stem cells. We report here the in vitro IC50 values of stem-like and non-stem XPA1 human pancreatic cells cells for: (1) 5-fluorouracil (5-FU), (2) cisplatinum (CDDP), (3) gemcitabine (GEM), and (4) tumor-targeting Salmonella typhimurium A1-R (A1-R). IC50 values of stem-like XPA1 cells were significantly higher than those of non-stem XPA1 cells for 5-FU (P = 0.007) and CDDP (P = 0.012). In contrast, there was no difference between the efficacy of A1-R on stem-like and non-stem XPA1 cells. In vivo, 5-FU and A1-R significantly reduced the tumor weight of non-stem XPA1 cells (5-FU; P = 0.028; A1-R; P = 0.011). In contrast, only A1-R significantly reduced tumor weight of stem-like XPA1 cells (P = 0.012). The combination of A1-R with 5-FU improved the antitumor efficacy compared with 5-FU monotherapy on the stem-like cells (P = 0.004). The results of the present report indicate A1-R is a promising therapy for chemo-resistant pancreatic cancer stem-like cells.
Original language | English |
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Pages (from-to) | 2774-2780 |
Number of pages | 7 |
Journal | Cell Cycle |
Volume | 12 |
Issue number | 17 |
DOIs | |
Publication status | Published - Sep 1 2013 |
Externally published | Yes |
Keywords
- Amino acid auxotrophy
- Chemoresistance
- Confocal microscopy
- Fluorescence imaging
- GFP
- Pancreatic cancer
- RFP
- Salmonella typhimurium
- Selective tumor targeting
- Stem cell
ASJC Scopus subject areas
- Molecular Biology
- Developmental Biology
- Cell Biology