Abstract
The XPA1 human pancreatic cancer cell line is dimorphic, with spindle stem-like cells and round non-stem cells. We report here the in vitro IC50 values of stem-like and non-stem XPA1 human pancreatic cells cells for: (1) 5-fluorouracil (5-FU), (2) cisplatinum (CDDP), (3) gemcitabine (GEM), and (4) tumor-targeting Salmonella typhimurium A1-R (A1-R). IC50 values of stem-like XPA1 cells were significantly higher than those of non-stem XPA1 cells for 5-FU (P = 0.007) and CDDP (P = 0.012). In contrast, there was no difference between the efficacy of A1-R on stem-like and non-stem XPA1 cells. In vivo, 5-FU and A1-R significantly reduced the tumor weight of non-stem XPA1 cells (5-FU; P = 0.028; A1-R; P = 0.011). In contrast, only A1-R significantly reduced tumor weight of stem-like XPA1 cells (P = 0.012). The combination of A1-R with 5-FU improved the antitumor efficacy compared with 5-FU monotherapy on the stem-like cells (P = 0.004). The results of the present report indicate A1-R is a promising therapy for chemo-resistant pancreatic cancer stem-like cells.
| Original language | English |
|---|---|
| Pages (from-to) | 2774-2780 |
| Number of pages | 7 |
| Journal | Cell Cycle |
| Volume | 12 |
| Issue number | 17 |
| DOIs | |
| Publication status | Published - Sep 1 2013 |
| Externally published | Yes |
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Keywords
- Amino acid auxotrophy
- Chemoresistance
- Confocal microscopy
- Fluorescence imaging
- GFP
- Pancreatic cancer
- RFP
- Salmonella typhimurium
- Selective tumor targeting
- Stem cell
ASJC Scopus subject areas
- Molecular Biology
- Developmental Biology
- Cell Biology
Cite this
Comparison of efficacy of Salmonella typhimurium A1-R and chemotherapy on stem-like and non-stem human pancreatic cancer cells. / Hiroshima, Yukihiko; Zhao, Ming; Zhang, Yong; Maawy, Ali; Hassanein, Mohamed K.; Uehara, Fuminari; Miwa, Shinji; Yano, Shuuya; Momiyama, Masashi; Suetsugu, Atsushi; Chishima, Takashi; Tanaka, Kuniya; Bouvet, Michael; Endo, Itaru; Hoffman, Robert M.
In: Cell Cycle, Vol. 12, No. 17, 01.09.2013, p. 2774-2780.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of efficacy of Salmonella typhimurium A1-R and chemotherapy on stem-like and non-stem human pancreatic cancer cells
AU - Hiroshima, Yukihiko
AU - Zhao, Ming
AU - Zhang, Yong
AU - Maawy, Ali
AU - Hassanein, Mohamed K.
AU - Uehara, Fuminari
AU - Miwa, Shinji
AU - Yano, Shuuya
AU - Momiyama, Masashi
AU - Suetsugu, Atsushi
AU - Chishima, Takashi
AU - Tanaka, Kuniya
AU - Bouvet, Michael
AU - Endo, Itaru
AU - Hoffman, Robert M.
PY - 2013/9/1
Y1 - 2013/9/1
N2 - The XPA1 human pancreatic cancer cell line is dimorphic, with spindle stem-like cells and round non-stem cells. We report here the in vitro IC50 values of stem-like and non-stem XPA1 human pancreatic cells cells for: (1) 5-fluorouracil (5-FU), (2) cisplatinum (CDDP), (3) gemcitabine (GEM), and (4) tumor-targeting Salmonella typhimurium A1-R (A1-R). IC50 values of stem-like XPA1 cells were significantly higher than those of non-stem XPA1 cells for 5-FU (P = 0.007) and CDDP (P = 0.012). In contrast, there was no difference between the efficacy of A1-R on stem-like and non-stem XPA1 cells. In vivo, 5-FU and A1-R significantly reduced the tumor weight of non-stem XPA1 cells (5-FU; P = 0.028; A1-R; P = 0.011). In contrast, only A1-R significantly reduced tumor weight of stem-like XPA1 cells (P = 0.012). The combination of A1-R with 5-FU improved the antitumor efficacy compared with 5-FU monotherapy on the stem-like cells (P = 0.004). The results of the present report indicate A1-R is a promising therapy for chemo-resistant pancreatic cancer stem-like cells.
AB - The XPA1 human pancreatic cancer cell line is dimorphic, with spindle stem-like cells and round non-stem cells. We report here the in vitro IC50 values of stem-like and non-stem XPA1 human pancreatic cells cells for: (1) 5-fluorouracil (5-FU), (2) cisplatinum (CDDP), (3) gemcitabine (GEM), and (4) tumor-targeting Salmonella typhimurium A1-R (A1-R). IC50 values of stem-like XPA1 cells were significantly higher than those of non-stem XPA1 cells for 5-FU (P = 0.007) and CDDP (P = 0.012). In contrast, there was no difference between the efficacy of A1-R on stem-like and non-stem XPA1 cells. In vivo, 5-FU and A1-R significantly reduced the tumor weight of non-stem XPA1 cells (5-FU; P = 0.028; A1-R; P = 0.011). In contrast, only A1-R significantly reduced tumor weight of stem-like XPA1 cells (P = 0.012). The combination of A1-R with 5-FU improved the antitumor efficacy compared with 5-FU monotherapy on the stem-like cells (P = 0.004). The results of the present report indicate A1-R is a promising therapy for chemo-resistant pancreatic cancer stem-like cells.
KW - Amino acid auxotrophy
KW - Chemoresistance
KW - Confocal microscopy
KW - Fluorescence imaging
KW - GFP
KW - Pancreatic cancer
KW - RFP
KW - Salmonella typhimurium
KW - Selective tumor targeting
KW - Stem cell
UR - http://www.scopus.com/inward/record.url?scp=84883575403&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883575403&partnerID=8YFLogxK
U2 - 10.4161/cc.25872
DO - 10.4161/cc.25872
M3 - Article
VL - 12
SP - 2774
EP - 2780
JO - Cell Cycle
JF - Cell Cycle
SN - 1538-4101
IS - 17
ER -