Comparison of cancer-cell seeding, viability and deformation in the lung, muscle and liver, visualized by subcellular real-time imaging in the live mouse

Katsuhiro Hayashi, Hiroaki Kimura, Kensuke Yamauchi, Norio Yamamoto, Hiroyuki Tsuchiya, Katsuro Tomita, Hiroyuki Kishimoto, Akihiro Hasegawa, Michael Bouvet, Robert M. Hoffman

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The comparison of cancer cell seeding, deformation and viability in the lung, muscle and liver of nude mice in real-time is reported here. The mice were intubated to support ventilation with positive end-respiratory pressure (PEEP) for imaging on the lung. Human fibrosarcoma cells with green fluorescent protein (GFP) in the nucleus and red fluorescent protein (RFP) in the cytoplasm (dual-color HT-1080 cells) were injected into the tail vein for lung imaging, the portal vein for liver imaging or the abdominal aorta for muscle imaging which was performed with an Olympus OV100 Small Animal Imaging System. The length of the cytoplasm and nuclei in 20 seeded cancer cells were measured. A large number of cells initially arrested in the lung capillaries and many cells formed aggregates. The cell number decreased rapidly at 6 and 24 h. There was no significant difference in cancer cell survival when immunocompetent C57BL/6 mice were used in place of the nude mice, suggesting that T cell reaction is not very important in the first 24 h after seeding of cancer cells in the lung. In the lung and liver, little cancer cell deformation occurred. In contrast in the muscle, the cytoplasm and nuclei of the seeded cells were highly deformed and many fragmented cells were observed. The rate of cancer cell death was highest in the lung and lowest in the muscle. In each organ, single disseminated cells tended to die earlier than aggregated cells. The results of this study suggest that the early steps of metastasis are different in the lung, liver and muscle.

Original languageEnglish
Pages (from-to)3665-3672
Number of pages8
JournalAnticancer research
Volume31
Issue number11
Publication statusPublished - Nov 2011

Keywords

  • Cancer cell seeding
  • Green fluorescent protein
  • Liver
  • Lung
  • Muscle
  • Real-time imaging
  • Red fluorescent protein

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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