Comparison of cancer-cell seeding, viability and deformation in the lung, muscle and liver, visualized by subcellular real-time imaging in the live mouse

Katsuhiro Hayashi, Hiroaki Kimura, Kensuke Yamauchi, Norio Yamamoto, Hiroyuki Tsuchiya, Katsuro Tomita, Hiroyuki Kishimoto, Akihiro Hasegawa, Michael Bouvet, Robert M. Hoffman

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The comparison of cancer cell seeding, deformation and viability in the lung, muscle and liver of nude mice in real-time is reported here. The mice were intubated to support ventilation with positive end-respiratory pressure (PEEP) for imaging on the lung. Human fibrosarcoma cells with green fluorescent protein (GFP) in the nucleus and red fluorescent protein (RFP) in the cytoplasm (dual-color HT-1080 cells) were injected into the tail vein for lung imaging, the portal vein for liver imaging or the abdominal aorta for muscle imaging which was performed with an Olympus OV100 Small Animal Imaging System. The length of the cytoplasm and nuclei in 20 seeded cancer cells were measured. A large number of cells initially arrested in the lung capillaries and many cells formed aggregates. The cell number decreased rapidly at 6 and 24 h. There was no significant difference in cancer cell survival when immunocompetent C57BL/6 mice were used in place of the nude mice, suggesting that T cell reaction is not very important in the first 24 h after seeding of cancer cells in the lung. In the lung and liver, little cancer cell deformation occurred. In contrast in the muscle, the cytoplasm and nuclei of the seeded cells were highly deformed and many fragmented cells were observed. The rate of cancer cell death was highest in the lung and lowest in the muscle. In each organ, single disseminated cells tended to die earlier than aggregated cells. The results of this study suggest that the early steps of metastasis are different in the lung, liver and muscle.

Original languageEnglish
Pages (from-to)3665-3672
Number of pages8
JournalAnticancer Research
Volume31
Issue number11
Publication statusPublished - Nov 2011

Fingerprint

Cell Survival
Muscles
Lung
Liver
Neoplasms
Cytoplasm
Nude Mice
Cell Count
Abdominal Muscles
Fibrosarcoma
Abdominal Aorta
Liver Neoplasms
Portal Vein
Green Fluorescent Proteins
Cell Nucleus
Inbred C57BL Mouse
Ventilation
Tail
Veins
Cell Death

Keywords

  • Cancer cell seeding
  • Green fluorescent protein
  • Liver
  • Lung
  • Muscle
  • Real-time imaging
  • Red fluorescent protein

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Hayashi, K., Kimura, H., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., ... Hoffman, R. M. (2011). Comparison of cancer-cell seeding, viability and deformation in the lung, muscle and liver, visualized by subcellular real-time imaging in the live mouse. Anticancer Research, 31(11), 3665-3672.

Comparison of cancer-cell seeding, viability and deformation in the lung, muscle and liver, visualized by subcellular real-time imaging in the live mouse. / Hayashi, Katsuhiro; Kimura, Hiroaki; Yamauchi, Kensuke; Yamamoto, Norio; Tsuchiya, Hiroyuki; Tomita, Katsuro; Kishimoto, Hiroyuki; Hasegawa, Akihiro; Bouvet, Michael; Hoffman, Robert M.

In: Anticancer Research, Vol. 31, No. 11, 11.2011, p. 3665-3672.

Research output: Contribution to journalArticle

Hayashi, K, Kimura, H, Yamauchi, K, Yamamoto, N, Tsuchiya, H, Tomita, K, Kishimoto, H, Hasegawa, A, Bouvet, M & Hoffman, RM 2011, 'Comparison of cancer-cell seeding, viability and deformation in the lung, muscle and liver, visualized by subcellular real-time imaging in the live mouse', Anticancer Research, vol. 31, no. 11, pp. 3665-3672.
Hayashi, Katsuhiro ; Kimura, Hiroaki ; Yamauchi, Kensuke ; Yamamoto, Norio ; Tsuchiya, Hiroyuki ; Tomita, Katsuro ; Kishimoto, Hiroyuki ; Hasegawa, Akihiro ; Bouvet, Michael ; Hoffman, Robert M. / Comparison of cancer-cell seeding, viability and deformation in the lung, muscle and liver, visualized by subcellular real-time imaging in the live mouse. In: Anticancer Research. 2011 ; Vol. 31, No. 11. pp. 3665-3672.
@article{1adedc2cbaeb4ba1a1f78e09ccf8964f,
title = "Comparison of cancer-cell seeding, viability and deformation in the lung, muscle and liver, visualized by subcellular real-time imaging in the live mouse",
abstract = "The comparison of cancer cell seeding, deformation and viability in the lung, muscle and liver of nude mice in real-time is reported here. The mice were intubated to support ventilation with positive end-respiratory pressure (PEEP) for imaging on the lung. Human fibrosarcoma cells with green fluorescent protein (GFP) in the nucleus and red fluorescent protein (RFP) in the cytoplasm (dual-color HT-1080 cells) were injected into the tail vein for lung imaging, the portal vein for liver imaging or the abdominal aorta for muscle imaging which was performed with an Olympus OV100 Small Animal Imaging System. The length of the cytoplasm and nuclei in 20 seeded cancer cells were measured. A large number of cells initially arrested in the lung capillaries and many cells formed aggregates. The cell number decreased rapidly at 6 and 24 h. There was no significant difference in cancer cell survival when immunocompetent C57BL/6 mice were used in place of the nude mice, suggesting that T cell reaction is not very important in the first 24 h after seeding of cancer cells in the lung. In the lung and liver, little cancer cell deformation occurred. In contrast in the muscle, the cytoplasm and nuclei of the seeded cells were highly deformed and many fragmented cells were observed. The rate of cancer cell death was highest in the lung and lowest in the muscle. In each organ, single disseminated cells tended to die earlier than aggregated cells. The results of this study suggest that the early steps of metastasis are different in the lung, liver and muscle.",
keywords = "Cancer cell seeding, Green fluorescent protein, Liver, Lung, Muscle, Real-time imaging, Red fluorescent protein",
author = "Katsuhiro Hayashi and Hiroaki Kimura and Kensuke Yamauchi and Norio Yamamoto and Hiroyuki Tsuchiya and Katsuro Tomita and Hiroyuki Kishimoto and Akihiro Hasegawa and Michael Bouvet and Hoffman, {Robert M.}",
year = "2011",
month = "11",
language = "English",
volume = "31",
pages = "3665--3672",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "11",

}

TY - JOUR

T1 - Comparison of cancer-cell seeding, viability and deformation in the lung, muscle and liver, visualized by subcellular real-time imaging in the live mouse

AU - Hayashi, Katsuhiro

AU - Kimura, Hiroaki

AU - Yamauchi, Kensuke

AU - Yamamoto, Norio

AU - Tsuchiya, Hiroyuki

AU - Tomita, Katsuro

AU - Kishimoto, Hiroyuki

AU - Hasegawa, Akihiro

AU - Bouvet, Michael

AU - Hoffman, Robert M.

PY - 2011/11

Y1 - 2011/11

N2 - The comparison of cancer cell seeding, deformation and viability in the lung, muscle and liver of nude mice in real-time is reported here. The mice were intubated to support ventilation with positive end-respiratory pressure (PEEP) for imaging on the lung. Human fibrosarcoma cells with green fluorescent protein (GFP) in the nucleus and red fluorescent protein (RFP) in the cytoplasm (dual-color HT-1080 cells) were injected into the tail vein for lung imaging, the portal vein for liver imaging or the abdominal aorta for muscle imaging which was performed with an Olympus OV100 Small Animal Imaging System. The length of the cytoplasm and nuclei in 20 seeded cancer cells were measured. A large number of cells initially arrested in the lung capillaries and many cells formed aggregates. The cell number decreased rapidly at 6 and 24 h. There was no significant difference in cancer cell survival when immunocompetent C57BL/6 mice were used in place of the nude mice, suggesting that T cell reaction is not very important in the first 24 h after seeding of cancer cells in the lung. In the lung and liver, little cancer cell deformation occurred. In contrast in the muscle, the cytoplasm and nuclei of the seeded cells were highly deformed and many fragmented cells were observed. The rate of cancer cell death was highest in the lung and lowest in the muscle. In each organ, single disseminated cells tended to die earlier than aggregated cells. The results of this study suggest that the early steps of metastasis are different in the lung, liver and muscle.

AB - The comparison of cancer cell seeding, deformation and viability in the lung, muscle and liver of nude mice in real-time is reported here. The mice were intubated to support ventilation with positive end-respiratory pressure (PEEP) for imaging on the lung. Human fibrosarcoma cells with green fluorescent protein (GFP) in the nucleus and red fluorescent protein (RFP) in the cytoplasm (dual-color HT-1080 cells) were injected into the tail vein for lung imaging, the portal vein for liver imaging or the abdominal aorta for muscle imaging which was performed with an Olympus OV100 Small Animal Imaging System. The length of the cytoplasm and nuclei in 20 seeded cancer cells were measured. A large number of cells initially arrested in the lung capillaries and many cells formed aggregates. The cell number decreased rapidly at 6 and 24 h. There was no significant difference in cancer cell survival when immunocompetent C57BL/6 mice were used in place of the nude mice, suggesting that T cell reaction is not very important in the first 24 h after seeding of cancer cells in the lung. In the lung and liver, little cancer cell deformation occurred. In contrast in the muscle, the cytoplasm and nuclei of the seeded cells were highly deformed and many fragmented cells were observed. The rate of cancer cell death was highest in the lung and lowest in the muscle. In each organ, single disseminated cells tended to die earlier than aggregated cells. The results of this study suggest that the early steps of metastasis are different in the lung, liver and muscle.

KW - Cancer cell seeding

KW - Green fluorescent protein

KW - Liver

KW - Lung

KW - Muscle

KW - Real-time imaging

KW - Red fluorescent protein

UR - http://www.scopus.com/inward/record.url?scp=83055179612&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=83055179612&partnerID=8YFLogxK

M3 - Article

C2 - 22110185

AN - SCOPUS:83055179612

VL - 31

SP - 3665

EP - 3672

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 11

ER -