Comparison of backscattered scanning electron microscopy and microradiography of secondary caries

G. W. Marshall, M. Staninec, Yasuhiro Torii, S. J. Marshall

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Carious lesions are usually studied using light microscopy and /or microradiography which require preparation of thin sections. Backscattered scanning electron microscopy (BSEM) has received little attention although it provides information similar to that obtained with microradiographs, with the potential for higher resolution. Recently, microscopes have been introduced that can be used to study wet or nonconducting specimens, offering techniques for studying specimens without desiccation or preparation of thin sections. This investigation sought to determine if secondary carious lesions have the same characteristics when studied by microradiography as when using 'wet' BSEM mode. Microradiographs were made of thin sections from restored teeth with secondary caries induced in an artificial caries system. The thin sections were also studied by BSEM with a partial pressure in the specimen chamber to prevent specimen charging. Comparisons of the lesion size and shape were made using the two methods. Lesion depth measurements in enamel were the same; lesions that penetrated into dentin appeared to be of similar size and shape, but lesion depths measured by BSEM were slightly greater (paired t-test, p

Original languageEnglish
Pages (from-to)1043-1050
Number of pages8
JournalScanning Microscopy
Volume3
Issue number4
Publication statusPublished - 1989
Externally publishedYes

Fingerprint

lesions
Scanning electron microscopy
scanning electron microscopy
Enamels
Partial pressure
Optical microscopy
depth measurement
preparation
Microscopes
enamels
teeth
drying
partial pressure
charging
chambers
microscopes
microscopy
high resolution

ASJC Scopus subject areas

  • Instrumentation

Cite this

Comparison of backscattered scanning electron microscopy and microradiography of secondary caries. / Marshall, G. W.; Staninec, M.; Torii, Yasuhiro; Marshall, S. J.

In: Scanning Microscopy, Vol. 3, No. 4, 1989, p. 1043-1050.

Research output: Contribution to journalArticle

Marshall, G. W. ; Staninec, M. ; Torii, Yasuhiro ; Marshall, S. J. / Comparison of backscattered scanning electron microscopy and microradiography of secondary caries. In: Scanning Microscopy. 1989 ; Vol. 3, No. 4. pp. 1043-1050.
@article{f644d9ec521a4177ab5c2facf36f03a5,
title = "Comparison of backscattered scanning electron microscopy and microradiography of secondary caries",
abstract = "Carious lesions are usually studied using light microscopy and /or microradiography which require preparation of thin sections. Backscattered scanning electron microscopy (BSEM) has received little attention although it provides information similar to that obtained with microradiographs, with the potential for higher resolution. Recently, microscopes have been introduced that can be used to study wet or nonconducting specimens, offering techniques for studying specimens without desiccation or preparation of thin sections. This investigation sought to determine if secondary carious lesions have the same characteristics when studied by microradiography as when using 'wet' BSEM mode. Microradiographs were made of thin sections from restored teeth with secondary caries induced in an artificial caries system. The thin sections were also studied by BSEM with a partial pressure in the specimen chamber to prevent specimen charging. Comparisons of the lesion size and shape were made using the two methods. Lesion depth measurements in enamel were the same; lesions that penetrated into dentin appeared to be of similar size and shape, but lesion depths measured by BSEM were slightly greater (paired t-test, p",
author = "Marshall, {G. W.} and M. Staninec and Yasuhiro Torii and Marshall, {S. J.}",
year = "1989",
language = "English",
volume = "3",
pages = "1043--1050",
journal = "Scanning Microscopy",
issn = "0891-7035",
publisher = "Scanning Microscopy International",
number = "4",

}

TY - JOUR

T1 - Comparison of backscattered scanning electron microscopy and microradiography of secondary caries

AU - Marshall, G. W.

AU - Staninec, M.

AU - Torii, Yasuhiro

AU - Marshall, S. J.

PY - 1989

Y1 - 1989

N2 - Carious lesions are usually studied using light microscopy and /or microradiography which require preparation of thin sections. Backscattered scanning electron microscopy (BSEM) has received little attention although it provides information similar to that obtained with microradiographs, with the potential for higher resolution. Recently, microscopes have been introduced that can be used to study wet or nonconducting specimens, offering techniques for studying specimens without desiccation or preparation of thin sections. This investigation sought to determine if secondary carious lesions have the same characteristics when studied by microradiography as when using 'wet' BSEM mode. Microradiographs were made of thin sections from restored teeth with secondary caries induced in an artificial caries system. The thin sections were also studied by BSEM with a partial pressure in the specimen chamber to prevent specimen charging. Comparisons of the lesion size and shape were made using the two methods. Lesion depth measurements in enamel were the same; lesions that penetrated into dentin appeared to be of similar size and shape, but lesion depths measured by BSEM were slightly greater (paired t-test, p

AB - Carious lesions are usually studied using light microscopy and /or microradiography which require preparation of thin sections. Backscattered scanning electron microscopy (BSEM) has received little attention although it provides information similar to that obtained with microradiographs, with the potential for higher resolution. Recently, microscopes have been introduced that can be used to study wet or nonconducting specimens, offering techniques for studying specimens without desiccation or preparation of thin sections. This investigation sought to determine if secondary carious lesions have the same characteristics when studied by microradiography as when using 'wet' BSEM mode. Microradiographs were made of thin sections from restored teeth with secondary caries induced in an artificial caries system. The thin sections were also studied by BSEM with a partial pressure in the specimen chamber to prevent specimen charging. Comparisons of the lesion size and shape were made using the two methods. Lesion depth measurements in enamel were the same; lesions that penetrated into dentin appeared to be of similar size and shape, but lesion depths measured by BSEM were slightly greater (paired t-test, p

UR - http://www.scopus.com/inward/record.url?scp=0024957266&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024957266&partnerID=8YFLogxK

M3 - Article

C2 - 2633330

AN - SCOPUS:0024957266

VL - 3

SP - 1043

EP - 1050

JO - Scanning Microscopy

JF - Scanning Microscopy

SN - 0891-7035

IS - 4

ER -