TY - JOUR
T1 - Comparison of a fluorogenic assay with a conventional method for rapid detection of Vibrio parahaermolyticus in seafoods
AU - Venkateswaran, Kasthuri
AU - Kurusu, Takashi
AU - Satake, Mikio
AU - Shinoda, Sumio
PY - 1996/9
Y1 - 1996/9
N2 - A conventional method and a fluorogenic assay for the detection of Vibrio parahaemolyticus were compared. Among 29 seafood samples examined for the presence of V. parahaemolyticus, 17 samples harbored V. parahaemolyticus, and trypsinlike activity was noticed in 19 seafoods. The added fluorogenic substrate was cleaved in single samples of shrimp, turbo, and cuttlefish from which V. parahaemolyticus could not be isolated by the conventional method. Vibrio alginolyticus, in addition to V. parahaemolyticus, was found to exhibit intracellular trypsinlike activity. Trypsinlike activity in seafoods was observed after the most probable number for the initial density of V. parahaemolyticus-like organisms was found to have reached >102 per g. A V. parahaemolyticus inoculum at 104 CFU/ml in arabinose-glucuronate medium was required to attain growth to l06 CFU/ml, which is the level necessary for the release of detectable amounts of fluorescent compound from the added substrate.
AB - A conventional method and a fluorogenic assay for the detection of Vibrio parahaemolyticus were compared. Among 29 seafood samples examined for the presence of V. parahaemolyticus, 17 samples harbored V. parahaemolyticus, and trypsinlike activity was noticed in 19 seafoods. The added fluorogenic substrate was cleaved in single samples of shrimp, turbo, and cuttlefish from which V. parahaemolyticus could not be isolated by the conventional method. Vibrio alginolyticus, in addition to V. parahaemolyticus, was found to exhibit intracellular trypsinlike activity. Trypsinlike activity in seafoods was observed after the most probable number for the initial density of V. parahaemolyticus-like organisms was found to have reached >102 per g. A V. parahaemolyticus inoculum at 104 CFU/ml in arabinose-glucuronate medium was required to attain growth to l06 CFU/ml, which is the level necessary for the release of detectable amounts of fluorescent compound from the added substrate.
UR - http://www.scopus.com/inward/record.url?scp=0029786677&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029786677&partnerID=8YFLogxK
U2 - 10.1128/aem.62.9.3516-3520.1996
DO - 10.1128/aem.62.9.3516-3520.1996
M3 - Article
C2 - 8795246
AN - SCOPUS:0029786677
VL - 62
SP - 3516
EP - 3520
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 9
ER -