Comparison between the characteristics of follicular fluid and the developmental competence of bovine oocytes

H. Iwata, J. Inoue, Koji Kimura, T. Kuge, T. Kuwayama, Y. Monji

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

There are great differences in the developmental competence of oocytes collected from individual cows. Oocytes grow and mature in the follicular fluid (FF). In the present study, characteristics of the FF of each ovary and the developmental competence of enclosed oocytes were investigated, and these data were then compared. A total of 37 pairs of ovaries were collected from beef heifers. The concentration of magnesium (Mg), aspirate aminotransferase (AST), nonesterified fatty acids (NEFA), and lactate dehydrogenase (LDH) in the FF were great compared with serum standard. Several significant correlations among these characteristics were detected. Forty-eight hours after fertilization, the stage of embryo development at an advanced developmental stage (>6 cell stage) is related to the rate of blastulation 8 days after fertilization. In addition, a significantly positive or negative correlation was observed between the developmental competence (the rate of cleavage in the embryo and blastulation) and the concentration of the icterus index (ICT) or blood urea nitrogen (BUN) in the FF. In conclusion, the quality of oocytes is affected by the environment in the follicle, and BUN or ICT is a predictable index of the developmental competence of oocytes.

Original languageEnglish
Pages (from-to)215-223
Number of pages9
JournalAnimal Reproduction Science
Volume91
Issue number3-4
DOIs
Publication statusPublished - Feb 2006
Externally publishedYes

Fingerprint

Follicular Fluid
follicular fluid
Mental Competency
Oocytes
oocytes
cattle
Blood Urea Nitrogen
urea nitrogen
Jaundice
Fertilization
Ovary
blood
transaminases
Transaminases
blood serum
lactate dehydrogenase
L-Lactate Dehydrogenase
Nonesterified Fatty Acids
beef cattle
Magnesium

Keywords

  • Cattle-ovarian follicular fluid
  • Development
  • Embryo

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biotechnology
  • Genetics

Cite this

Comparison between the characteristics of follicular fluid and the developmental competence of bovine oocytes. / Iwata, H.; Inoue, J.; Kimura, Koji; Kuge, T.; Kuwayama, T.; Monji, Y.

In: Animal Reproduction Science, Vol. 91, No. 3-4, 02.2006, p. 215-223.

Research output: Contribution to journalArticle

Iwata, H. ; Inoue, J. ; Kimura, Koji ; Kuge, T. ; Kuwayama, T. ; Monji, Y. / Comparison between the characteristics of follicular fluid and the developmental competence of bovine oocytes. In: Animal Reproduction Science. 2006 ; Vol. 91, No. 3-4. pp. 215-223.
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