Comparative transcriptome profiling of two Tibetan wild barley genotypes in responses to low potassium

Jianbin Zeng, Xiaoyan He, Dezhi Wu, Bo Zhu, Shengguan Cai, Umme Aktari Nadira, Zahra Jabeen, Guoping Zhang

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Potassium (K) deficiency is one of the major factors affecting crop growth and productivity. Development of low-K tolerant crops is an effective approach to solve the nutritional deficiency in agricultural production. Tibetan annual wild barley is rich in genetic diversity and can grow normally under poor soils, including low-K supply. However, the molecular mechanism about low K tolerance is still poorly understood. In this study, Illumina RNA-Sequencing was performed using two Tibetan wild barley genotypes differing in low K tolerance (XZ153, tolerant and XZ141, sensitive), to determine the genotypic difference in transcriptome profiling. We identified a total of 692 differentially expressed genes (DEGs) in two genotypes at 6 h and 48 h after low-K treatment, including transcription factors, transporters and kinases, oxidative stress and hormone signaling related genes. Meanwhile, 294 low-K tolerant associated DEGs were assigned to transporter and antioxidant activities, stimulus response, and other gene ontology (GO), which were mainly involved in starch and sucrose metabolism, lipid metabolism and ethylene biosynthesis. Finally, a hypothetical model of low-K tolerance mechanism in XZ153 was presented. It may be concluded that wild barley accession XZ153 has a higher capability of K absorption and use efficiency than XZ141 under low K stress. A rapid response to low K stress in XZ153 is attributed to its more K uptake and accumulation in plants, resulting in higher low K tolerance. The ethylene response pathway may account for the genotypic difference in low-K tolerance.

Original languageEnglish
Article numbere100567
JournalPLoS One
Volume9
Issue number6
DOIs
Publication statusPublished - Jun 20 2014
Externally publishedYes

Fingerprint

Gene Expression Profiling
Hordeum
transcriptomics
Potassium
barley
Genotype
potassium
Genes
genotype
Potassium Deficiency
RNA Sequence Analysis
transporters
Gene Ontology
genes
Crops
Lipid Metabolism
Malnutrition
Starch
Sucrose
Oxidative Stress

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Comparative transcriptome profiling of two Tibetan wild barley genotypes in responses to low potassium. / Zeng, Jianbin; He, Xiaoyan; Wu, Dezhi; Zhu, Bo; Cai, Shengguan; Nadira, Umme Aktari; Jabeen, Zahra; Zhang, Guoping.

In: PLoS One, Vol. 9, No. 6, e100567, 20.06.2014.

Research output: Contribution to journalArticle

Zeng, J, He, X, Wu, D, Zhu, B, Cai, S, Nadira, UA, Jabeen, Z & Zhang, G 2014, 'Comparative transcriptome profiling of two Tibetan wild barley genotypes in responses to low potassium', PLoS One, vol. 9, no. 6, e100567. https://doi.org/10.1371/journal.pone.0100567
Zeng, Jianbin ; He, Xiaoyan ; Wu, Dezhi ; Zhu, Bo ; Cai, Shengguan ; Nadira, Umme Aktari ; Jabeen, Zahra ; Zhang, Guoping. / Comparative transcriptome profiling of two Tibetan wild barley genotypes in responses to low potassium. In: PLoS One. 2014 ; Vol. 9, No. 6.
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