TY - JOUR
T1 - Comparative transcriptome analysis of green/white variegated sectors in Arabidopsis yellow variegated2
T2 - Responses to oxidative and other stresses in white sectors
AU - Miura, Eiko
AU - Kato, Yusuke
AU - Sakamoto, Wataru
N1 - Funding Information:
The authors would like to thank Dr S Shigeoka (Kinki University) for sAPX and tAPX antibodies, Dr T Shikanai (Kyoto University) for FSD and plastocyanin antibodies, Arabidopsis CSD2ox-2 (CSD2ox), Dr K Goto (Okayama Research Institute of Biological Institute) for help with GeneSpring GX 10 software, and Dr K Rikiishi (Okayama University) for help with real-time RT-PCR analysis. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) (16085207 and 19657017), the Asahi Glass Foundation, and the Oohara Foundation. EM is supported by a postdoctoral fellowship from the Japan Society for the Promotion of Science (JSPS).
PY - 2010/5
Y1 - 2010/5
N2 - The yellow variegated2 (var2) mutant in Arabidopsis thaliana has been studied as a typical leaf-variegated mutant whose defect results from the lack of FtsH2 metalloprotease in chloroplasts. The var2 green sectors suffer from photo-oxidative stress and accumulate high levels of reactive oxygen species (ROS) because of compromised Photosystem II repair. This study investigated and compared microarray-based expression profiles of green and white sectors of var2 leaves. Results suggest that ROS that accumulate in chloroplasts of var2 green sectors do not cause much significant change in the transcriptional profile related to ROS signalling and scavenging. By contrast, transcriptome in the white sectors apparently differs from those in the green sectors and wild type. Numerous genes related to photosynthesis and chloroplast functions were repressed in the white sectors. Furthermore, many genes related to oxidative stress were up-regulated. Among them, ROS scavenging genes were specifically examined, such as Cu/Zn superoxide dismutase 2 (CSD2), that were apparently up-regulated in white but not in the green sectors. Up-regulation of CSD2 appears to be partly attributable to the lack of a microRNA (miR398) in the white sectors. It was concluded that the white sectors exhibit a response to oxidative and other stresses, including CSD2 up-regulation, which might be commonly found in tissues with abnormal chloroplast differentiation.
AB - The yellow variegated2 (var2) mutant in Arabidopsis thaliana has been studied as a typical leaf-variegated mutant whose defect results from the lack of FtsH2 metalloprotease in chloroplasts. The var2 green sectors suffer from photo-oxidative stress and accumulate high levels of reactive oxygen species (ROS) because of compromised Photosystem II repair. This study investigated and compared microarray-based expression profiles of green and white sectors of var2 leaves. Results suggest that ROS that accumulate in chloroplasts of var2 green sectors do not cause much significant change in the transcriptional profile related to ROS signalling and scavenging. By contrast, transcriptome in the white sectors apparently differs from those in the green sectors and wild type. Numerous genes related to photosynthesis and chloroplast functions were repressed in the white sectors. Furthermore, many genes related to oxidative stress were up-regulated. Among them, ROS scavenging genes were specifically examined, such as Cu/Zn superoxide dismutase 2 (CSD2), that were apparently up-regulated in white but not in the green sectors. Up-regulation of CSD2 appears to be partly attributable to the lack of a microRNA (miR398) in the white sectors. It was concluded that the white sectors exhibit a response to oxidative and other stresses, including CSD2 up-regulation, which might be commonly found in tissues with abnormal chloroplast differentiation.
KW - Arabidopsis
KW - Chloroplasts
KW - MiR398
KW - Plastids
KW - Reactive oxygen species (ROS)
KW - Superoxide dismutase (SOD)
KW - Yellow variegated2 (var2)
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U2 - 10.1093/jxb/erq075
DO - 10.1093/jxb/erq075
M3 - Article
C2 - 20400527
AN - SCOPUS:77952970809
VL - 61
SP - 2433
EP - 2445
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
SN - 0022-0957
IS - 9
ER -