Comparative study of the membrane-permeabilizing activities of mastoparans and related histamine-releasing agents in bacteria, erythrocytes, and mast cells

Satoshi Nakao, Keiko Komagoe, Tsuyoshi Inoue, Takashi Katsu

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The membrane-permeabilizing activities of mastoparans and related histamine-releasing agents were compared through measurements of K+ efflux from bacteria, erythrocytes, and mast cells. Changes in bacterial cell viability, hemolysis, and histamine release, as well as in the shape of erythrocytes were also investigated. The compounds tested were mastoparans (HR1, a mastoparan from Polistes jadwagae, and a mastoparan from Vespula lewisii), granuliberin R, mast cell-degranulating peptide, and compound 48/80, as well as antimicrobial peptides, such as magainin I, magainin II, gramicidin S, and melittin. We used a K+-selective electrode to determine changes in the permeability to K+ of the cytoplasmic membranes of cells. Consistent with the surface of mast cells becoming negatively charged during histamine release, due to the translocation of phosphatidylserine to the outer leaflet of the cytoplasmic membrane, histamine-releasing agents induced K + efflux from mast cells, dependent on their ability to increase the permeability of bacterial cytoplasmic membranes rich in negatively charged phospholipids. The present results demonstrated that amphiphilic peptides, possessing both histamine-releasing and antimicrobial capabilities, induced the permeabilization of the cytoplasmic membranes of not only bacteria but mast cells. Mastoparans increased the permeability of membranes in human erythrocytes at higher concentrations, and changed the normal discoid shape to a crenated form. The structural requirement for making the crenated form was determined using compound 48/80 and its constituents (monomer, dimer, and trimer), changing systematically the number of cationic charges of the molecules.

Original languageEnglish
Pages (from-to)490-497
Number of pages8
JournalBBA - Biomembranes
Volume1808
Issue number1
DOIs
Publication statusPublished - Jan 2011

Fingerprint

Histamine Agents
Mast Cells
Histamine
Bacteria
Magainins
Erythrocytes
Cell Membrane
Membranes
p-Methoxy-N-methylphenethylamine
Permeability
Histamine Release
Melitten
Microbial Viability
Gramicidin
Peptides
Phosphatidylserines
Hemolysis
Phospholipids
Cell Survival
Electrodes

Keywords

  • Antimicrobial peptide
  • Cell shape
  • Compound 48/80
  • Histamine-releasing agent
  • Mastoparan
  • Membrane permeability

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Cite this

Comparative study of the membrane-permeabilizing activities of mastoparans and related histamine-releasing agents in bacteria, erythrocytes, and mast cells. / Nakao, Satoshi; Komagoe, Keiko; Inoue, Tsuyoshi; Katsu, Takashi.

In: BBA - Biomembranes, Vol. 1808, No. 1, 01.2011, p. 490-497.

Research output: Contribution to journalArticle

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