Comparative studies on the late bleaching processes of four kinds of cone visual pigments and rod visual pigment

Keita Sato, Takahiro Yamashita, Yasushi Imamoto, Yoshinori Shichida

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Visual pigments in rod and cone photoreceptor cells of vertebrate retinas are highly diversified photoreceptive proteins that consist of a protein moiety opsin and a light-absorbing chromophore 11-cis-retinal. There are four types of cone visual pigments and a single type of rod visual pigment. The reaction process of the rod visual pigment, rhodopsin, has been extensively investigated, whereas there have been few studies of cone visual pigments. Here we comprehensively investigated the reaction processes of cone visual pigments on a time scale of milliseconds to minutes, using flash photolysis equipment optimized for cone visual pigment photochemistry. We used chicken violet (L-group), chicken blue (M1-group), chicken green (M2-group), and monkey green (L-group) visual pigments as representatives of the respective groups of the phylogenetic tree of cone pigments. The S, M1, and M2 pigments showed the formation of a pH-dependent mixture of meta intermediates, similar to that formed from rhodopsin. Although monkey green (L-group) also formed a mixture of meta intermediates, pH dependency of meta intermediates was not observed. However, meta intermediates of monkey green became pH dependent when the chloride ion bound to the monkey green was replaced with a nitrate ion. These results strongly suggest that rhodopsin and S, M1, and M2 cone visual pigments share a molecular mechanism for activation, whereas the L-group pigment may have a special reaction mechanism involving the chloride-binding site.

Original languageEnglish
Pages (from-to)4300-4308
Number of pages9
JournalBiochemistry
Volume51
Issue number21
DOIs
Publication statusPublished - May 29 2012
Externally publishedYes

Fingerprint

Retinal Pigments
Bleaching
Cones
Cercopithecus aethiops
Rhodopsin
Pigments
Chickens
Chlorides
Ions
Retinaldehyde
Retinal Cone Photoreceptor Cells
Opsins
Retinal Rod Photoreceptor Cells
Photochemistry
Vertebrate Photoreceptor Cells
Photolysis
Photochemical reactions
Chromophores
Nitrates
Vertebrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Comparative studies on the late bleaching processes of four kinds of cone visual pigments and rod visual pigment. / Sato, Keita; Yamashita, Takahiro; Imamoto, Yasushi; Shichida, Yoshinori.

In: Biochemistry, Vol. 51, No. 21, 29.05.2012, p. 4300-4308.

Research output: Contribution to journalArticle

Sato, Keita ; Yamashita, Takahiro ; Imamoto, Yasushi ; Shichida, Yoshinori. / Comparative studies on the late bleaching processes of four kinds of cone visual pigments and rod visual pigment. In: Biochemistry. 2012 ; Vol. 51, No. 21. pp. 4300-4308.
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