Comparative studies on naturally occurring antikeratin antibodies in human sera

Keiji Iwatsuki, Jacqueline Viac, Alain Reano, Anna Morera, Marie Jeanne Staquet, Jean Thivolet, Jean Claude Monier

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Comparative studies on the specificity of the so-called antiepidermal antibodies (Abs) found in human sera were performed by immunoblotting, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy (IEM). After a screening test by indirect immunofluorescence (IF), sera obtained from patients with various diseases and controls could be classified in 5 different groups according to the IF patterns on the epidermis: sera reactive with: (1) the stratum corneum (SC); (2) the upper layer (U-Cyt); (3) the whole epidermis (G-Cyt); (4) basal cells (B-Cyt); and (5) negative ones. By immunoblotting, all the 23 IF-positive sera were found to bind to one or more keratin bands, and did not show any reactivity with epidermal Nonidet P-40 soluble proteins. SC-Abs were mainly directed against a 67 kD Keratin band, whereas U-Cyt-and G-Cyt-Abs bound to both 58-56 kD and 67-63 kD keratins. B-Cyt-Abs reacted strongly with 63 kD keratins and slightly with a 50 kD band. Antikeratin Abs were detected by immunoblotting even in the IF-negative sera. The ELISA study showed that sera with high IF titers contained high levels of antikeratin Abs. In the IEM study using sera containing U-Cyt- or B-Cyt-Abs, 2 distinct reaction patterns were demonstrated: U-Cyt-Abs stained tonofilaments of suprabasal keratinocytes, while B-Cyt-Abs characteristically reacted with those of basal cells. Moreover, SC-, U-Cyt-, and G-Cyt-Abs were absorbed out by insoluble epidermal proteins, and B-Cyt-Abs were decreased in titer after the absorption test. The present study provides strong evidence that most, though not all, human antiepidermal Abs are directed against different keratin polypeptides, and that antikeratin Abs commonly occur in almost all human sera.

Original languageEnglish
Pages (from-to)179-184
Number of pages6
JournalJournal of Investigative Dermatology
Volume87
Issue number2
Publication statusPublished - Aug 1986
Externally publishedYes

Fingerprint

Antibodies
Serum
Keratins
Fluorescent Antibody Technique
Immunoblotting
Cornea
Immunosorbents
Immunoelectron Microscopy
Epidermis
Assays
Microscopic examination
Enzyme-Linked Immunosorbent Assay
Cells
Intermediate Filaments
Enzymes
Indirect Fluorescent Antibody Technique
Keratinocytes
Screening
Peptides
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Iwatsuki, K., Viac, J., Reano, A., Morera, A., Staquet, M. J., Thivolet, J., & Monier, J. C. (1986). Comparative studies on naturally occurring antikeratin antibodies in human sera. Journal of Investigative Dermatology, 87(2), 179-184.

Comparative studies on naturally occurring antikeratin antibodies in human sera. / Iwatsuki, Keiji; Viac, Jacqueline; Reano, Alain; Morera, Anna; Staquet, Marie Jeanne; Thivolet, Jean; Monier, Jean Claude.

In: Journal of Investigative Dermatology, Vol. 87, No. 2, 08.1986, p. 179-184.

Research output: Contribution to journalArticle

Iwatsuki, K, Viac, J, Reano, A, Morera, A, Staquet, MJ, Thivolet, J & Monier, JC 1986, 'Comparative studies on naturally occurring antikeratin antibodies in human sera', Journal of Investigative Dermatology, vol. 87, no. 2, pp. 179-184.
Iwatsuki K, Viac J, Reano A, Morera A, Staquet MJ, Thivolet J et al. Comparative studies on naturally occurring antikeratin antibodies in human sera. Journal of Investigative Dermatology. 1986 Aug;87(2):179-184.
Iwatsuki, Keiji ; Viac, Jacqueline ; Reano, Alain ; Morera, Anna ; Staquet, Marie Jeanne ; Thivolet, Jean ; Monier, Jean Claude. / Comparative studies on naturally occurring antikeratin antibodies in human sera. In: Journal of Investigative Dermatology. 1986 ; Vol. 87, No. 2. pp. 179-184.
@article{ef72e764ee2f4da49039247ac26b8625,
title = "Comparative studies on naturally occurring antikeratin antibodies in human sera",
abstract = "Comparative studies on the specificity of the so-called antiepidermal antibodies (Abs) found in human sera were performed by immunoblotting, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy (IEM). After a screening test by indirect immunofluorescence (IF), sera obtained from patients with various diseases and controls could be classified in 5 different groups according to the IF patterns on the epidermis: sera reactive with: (1) the stratum corneum (SC); (2) the upper layer (U-Cyt); (3) the whole epidermis (G-Cyt); (4) basal cells (B-Cyt); and (5) negative ones. By immunoblotting, all the 23 IF-positive sera were found to bind to one or more keratin bands, and did not show any reactivity with epidermal Nonidet P-40 soluble proteins. SC-Abs were mainly directed against a 67 kD Keratin band, whereas U-Cyt-and G-Cyt-Abs bound to both 58-56 kD and 67-63 kD keratins. B-Cyt-Abs reacted strongly with 63 kD keratins and slightly with a 50 kD band. Antikeratin Abs were detected by immunoblotting even in the IF-negative sera. The ELISA study showed that sera with high IF titers contained high levels of antikeratin Abs. In the IEM study using sera containing U-Cyt- or B-Cyt-Abs, 2 distinct reaction patterns were demonstrated: U-Cyt-Abs stained tonofilaments of suprabasal keratinocytes, while B-Cyt-Abs characteristically reacted with those of basal cells. Moreover, SC-, U-Cyt-, and G-Cyt-Abs were absorbed out by insoluble epidermal proteins, and B-Cyt-Abs were decreased in titer after the absorption test. The present study provides strong evidence that most, though not all, human antiepidermal Abs are directed against different keratin polypeptides, and that antikeratin Abs commonly occur in almost all human sera.",
author = "Keiji Iwatsuki and Jacqueline Viac and Alain Reano and Anna Morera and Staquet, {Marie Jeanne} and Jean Thivolet and Monier, {Jean Claude}",
year = "1986",
month = "8",
language = "English",
volume = "87",
pages = "179--184",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Comparative studies on naturally occurring antikeratin antibodies in human sera

AU - Iwatsuki, Keiji

AU - Viac, Jacqueline

AU - Reano, Alain

AU - Morera, Anna

AU - Staquet, Marie Jeanne

AU - Thivolet, Jean

AU - Monier, Jean Claude

PY - 1986/8

Y1 - 1986/8

N2 - Comparative studies on the specificity of the so-called antiepidermal antibodies (Abs) found in human sera were performed by immunoblotting, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy (IEM). After a screening test by indirect immunofluorescence (IF), sera obtained from patients with various diseases and controls could be classified in 5 different groups according to the IF patterns on the epidermis: sera reactive with: (1) the stratum corneum (SC); (2) the upper layer (U-Cyt); (3) the whole epidermis (G-Cyt); (4) basal cells (B-Cyt); and (5) negative ones. By immunoblotting, all the 23 IF-positive sera were found to bind to one or more keratin bands, and did not show any reactivity with epidermal Nonidet P-40 soluble proteins. SC-Abs were mainly directed against a 67 kD Keratin band, whereas U-Cyt-and G-Cyt-Abs bound to both 58-56 kD and 67-63 kD keratins. B-Cyt-Abs reacted strongly with 63 kD keratins and slightly with a 50 kD band. Antikeratin Abs were detected by immunoblotting even in the IF-negative sera. The ELISA study showed that sera with high IF titers contained high levels of antikeratin Abs. In the IEM study using sera containing U-Cyt- or B-Cyt-Abs, 2 distinct reaction patterns were demonstrated: U-Cyt-Abs stained tonofilaments of suprabasal keratinocytes, while B-Cyt-Abs characteristically reacted with those of basal cells. Moreover, SC-, U-Cyt-, and G-Cyt-Abs were absorbed out by insoluble epidermal proteins, and B-Cyt-Abs were decreased in titer after the absorption test. The present study provides strong evidence that most, though not all, human antiepidermal Abs are directed against different keratin polypeptides, and that antikeratin Abs commonly occur in almost all human sera.

AB - Comparative studies on the specificity of the so-called antiepidermal antibodies (Abs) found in human sera were performed by immunoblotting, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy (IEM). After a screening test by indirect immunofluorescence (IF), sera obtained from patients with various diseases and controls could be classified in 5 different groups according to the IF patterns on the epidermis: sera reactive with: (1) the stratum corneum (SC); (2) the upper layer (U-Cyt); (3) the whole epidermis (G-Cyt); (4) basal cells (B-Cyt); and (5) negative ones. By immunoblotting, all the 23 IF-positive sera were found to bind to one or more keratin bands, and did not show any reactivity with epidermal Nonidet P-40 soluble proteins. SC-Abs were mainly directed against a 67 kD Keratin band, whereas U-Cyt-and G-Cyt-Abs bound to both 58-56 kD and 67-63 kD keratins. B-Cyt-Abs reacted strongly with 63 kD keratins and slightly with a 50 kD band. Antikeratin Abs were detected by immunoblotting even in the IF-negative sera. The ELISA study showed that sera with high IF titers contained high levels of antikeratin Abs. In the IEM study using sera containing U-Cyt- or B-Cyt-Abs, 2 distinct reaction patterns were demonstrated: U-Cyt-Abs stained tonofilaments of suprabasal keratinocytes, while B-Cyt-Abs characteristically reacted with those of basal cells. Moreover, SC-, U-Cyt-, and G-Cyt-Abs were absorbed out by insoluble epidermal proteins, and B-Cyt-Abs were decreased in titer after the absorption test. The present study provides strong evidence that most, though not all, human antiepidermal Abs are directed against different keratin polypeptides, and that antikeratin Abs commonly occur in almost all human sera.

UR - http://www.scopus.com/inward/record.url?scp=0022517704&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022517704&partnerID=8YFLogxK

M3 - Article

VL - 87

SP - 179

EP - 184

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 2

ER -