Comparative mapping of the ASTRINGENCY locus controlling fruit astringency in hexaploid persimmon (Diospyros kaki thunb.) with the diploid D. lotus reference genome

Soichiro Nishiyama, Noriyuki Onoue, Atsushi Kono, Akihiko Sato, Koichiro Ushijima, Hisayo Yamane, Ryutaro Tao, Keizo Yonemori

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Persimmon (Diospyros kaki) is a tree crop species that originated in East Asia, consists mainly of hexaploid individuals (2n = 6x = 90) with some nonaploid individuals. One of the unique characteristics of persimmon is the continuous accumulation of proanthocyanidins (PAs) in its fruit until the middle of fruit development, resulting in a strong astringent taste even at commercial fruit maturity. Among persimmon cultivars, pollination-constant and non-astringent (PCNA) types cease PA accumulation in early fruit development and become non-astringent at commercial maturity. PCNA is an allelic trait to non-PCNA and is controlled by a single locus called the ASTRINGENCY (AST) locus. Previous segregation analyses indicated that the AST locus shows hexasomic inheritance; a recessive allele, ast, at this locus confers PCNA. Here, we report a shuttle mapping approach to delimit the AST locus region in the hexaploid persimmon genome by using D. lotus, a diploid relative of D. kaki, as a reference. A D. lotus F1 population of 333 individuals and 296 D. kaki siblings segregating for the PCNA trait were used to map the AST region using haplotype-specific markers covering the AST region. This indicated that the AST locus is syntenic to an approximately 915-kb region of the D. lotus genome. In this 915-kb region, we found several candidates for AST that were revealed from the fruit transcriptome of a population segregating for the PCNA trait. These results could provide important clues for the isolation of AST in hexaploid persimmon.

Original languageEnglish
Pages (from-to)315-323
Number of pages9
JournalHorticulture Journal
Volume87
Issue number3
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Diospyros lotus
Diospyros kaki
astringency
persimmons
hexaploidy
diploidy
pollination
loci
fruits
genome
Lotus
proanthocyanidins
fruiting
fruit maturity
East Asia
transcriptome
inheritance (genetics)
haplotypes
alleles
cultivars

Keywords

  • Candidate gene
  • Chromosome walking
  • Polyploidy
  • Proanthocyanidins

ASJC Scopus subject areas

  • Plant Science
  • Horticulture

Cite this

Comparative mapping of the ASTRINGENCY locus controlling fruit astringency in hexaploid persimmon (Diospyros kaki thunb.) with the diploid D. lotus reference genome. / Nishiyama, Soichiro; Onoue, Noriyuki; Kono, Atsushi; Sato, Akihiko; Ushijima, Koichiro; Yamane, Hisayo; Tao, Ryutaro; Yonemori, Keizo.

In: Horticulture Journal, Vol. 87, No. 3, 01.01.2018, p. 315-323.

Research output: Contribution to journalArticle

Nishiyama, Soichiro ; Onoue, Noriyuki ; Kono, Atsushi ; Sato, Akihiko ; Ushijima, Koichiro ; Yamane, Hisayo ; Tao, Ryutaro ; Yonemori, Keizo. / Comparative mapping of the ASTRINGENCY locus controlling fruit astringency in hexaploid persimmon (Diospyros kaki thunb.) with the diploid D. lotus reference genome. In: Horticulture Journal. 2018 ; Vol. 87, No. 3. pp. 315-323.
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AU - Sato, Akihiko

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KW - Candidate gene

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