TY - JOUR
T1 - Comparative mapping of the ASTRINGENCY locus controlling fruit astringency in hexaploid persimmon (Diospyros kaki thunb.) with the diploid D. lotus reference genome
AU - Nishiyama, Soichiro
AU - Onoue, Noriyuki
AU - Kono, Atsushi
AU - Sato, Akihiko
AU - Ushijima, Koichiro
AU - Yamane, Hisayo
AU - Tao, Ryutaro
AU - Yonemori, Keizo
N1 - Funding Information:
Received; September 22, 2017. Accepted; November 24, 2017. First Published Online in J-STAGE on February 1, 2018. Part of this study was presented at the 2015 Autumn Meeting and the 2017 Autumn Meeting of the Japanese Society for Horticultural Science. This work was supported by a Grant-in-Aid for JSPS Research Fellow (Grant Number JP16J10408), and for Scientific Research (B) (Grant Number JP16H04876) from the Japan Society for the Promotion of Science. The authors declare that they have no conflicts of interest. * Corresponding author (E-mail: keizo@agr.ryukoku.ac.jp). ** Present address: Faculty of Agriculture, Ryukoku University, Otsu 520-2194, Japan.
Publisher Copyright:
© 2018 The Japanese Society for Horticultural Science (JSHS), All rights reserved.
PY - 2018
Y1 - 2018
N2 - Persimmon (Diospyros kaki) is a tree crop species that originated in East Asia, consists mainly of hexaploid individuals (2n = 6x = 90) with some nonaploid individuals. One of the unique characteristics of persimmon is the continuous accumulation of proanthocyanidins (PAs) in its fruit until the middle of fruit development, resulting in a strong astringent taste even at commercial fruit maturity. Among persimmon cultivars, pollination-constant and non-astringent (PCNA) types cease PA accumulation in early fruit development and become non-astringent at commercial maturity. PCNA is an allelic trait to non-PCNA and is controlled by a single locus called the ASTRINGENCY (AST) locus. Previous segregation analyses indicated that the AST locus shows hexasomic inheritance; a recessive allele, ast, at this locus confers PCNA. Here, we report a shuttle mapping approach to delimit the AST locus region in the hexaploid persimmon genome by using D. lotus, a diploid relative of D. kaki, as a reference. A D. lotus F1 population of 333 individuals and 296 D. kaki siblings segregating for the PCNA trait were used to map the AST region using haplotype-specific markers covering the AST region. This indicated that the AST locus is syntenic to an approximately 915-kb region of the D. lotus genome. In this 915-kb region, we found several candidates for AST that were revealed from the fruit transcriptome of a population segregating for the PCNA trait. These results could provide important clues for the isolation of AST in hexaploid persimmon.
AB - Persimmon (Diospyros kaki) is a tree crop species that originated in East Asia, consists mainly of hexaploid individuals (2n = 6x = 90) with some nonaploid individuals. One of the unique characteristics of persimmon is the continuous accumulation of proanthocyanidins (PAs) in its fruit until the middle of fruit development, resulting in a strong astringent taste even at commercial fruit maturity. Among persimmon cultivars, pollination-constant and non-astringent (PCNA) types cease PA accumulation in early fruit development and become non-astringent at commercial maturity. PCNA is an allelic trait to non-PCNA and is controlled by a single locus called the ASTRINGENCY (AST) locus. Previous segregation analyses indicated that the AST locus shows hexasomic inheritance; a recessive allele, ast, at this locus confers PCNA. Here, we report a shuttle mapping approach to delimit the AST locus region in the hexaploid persimmon genome by using D. lotus, a diploid relative of D. kaki, as a reference. A D. lotus F1 population of 333 individuals and 296 D. kaki siblings segregating for the PCNA trait were used to map the AST region using haplotype-specific markers covering the AST region. This indicated that the AST locus is syntenic to an approximately 915-kb region of the D. lotus genome. In this 915-kb region, we found several candidates for AST that were revealed from the fruit transcriptome of a population segregating for the PCNA trait. These results could provide important clues for the isolation of AST in hexaploid persimmon.
KW - Candidate gene
KW - Chromosome walking
KW - Polyploidy
KW - Proanthocyanidins
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U2 - 10.2503/hortj.OKD-140
DO - 10.2503/hortj.OKD-140
M3 - Article
AN - SCOPUS:85050949397
VL - 87
SP - 315
EP - 323
JO - Horticulture Journal
JF - Horticulture Journal
SN - 2189-0102
IS - 3
ER -