Comparative genomic analysis of Lactococcus garvieae strains isolated from different sources reveals candidate virulence genes

Eiji Miyauchi, Hidehiro Toh, Akiyo Nakano, Soichi Tanabe, Hidetoshi Morita

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Lactococcus garvieae is a major pathogen for fish. Two complete (ATCC 49156 and Lg2) and three draft (UNIUD074, 8831, and 21881) genome sequences of L. garvieae have recently been released. We here present the results of a comparative genomic analysis of these fish and human isolates of L. garvieae. The pangenome comprised 1,542 core and 1,378 dispensable genes. The sequenced L. garvieae strains shared most of the possible virulence genes, but the capsule gene cluster was found only in fish-pathogenic strain Lg2. The absence of the capsule gene cluster in other nonpathogenic strains isolated from mastitis and vegetable was also confirmed by PCR. The fish and human isolates of L. garvieae contained the specific two and four adhesin genes, respectively, indicating that these adhesion proteins may be involved in the host specificity differences of L. garvieae. The discoveries revealed by the pangenomic analysis may provide significant insights into the biology of L. garvieae.

Original languageEnglish
Article number728276
JournalInternational Journal of Microbiology
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Lactococcus
Virulence
Fishes
Multigene Family
Genes
Capsules
Mastitis
Host Specificity
Vegetables
Genome
Polymerase Chain Reaction
Proteins

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Comparative genomic analysis of Lactococcus garvieae strains isolated from different sources reveals candidate virulence genes. / Miyauchi, Eiji; Toh, Hidehiro; Nakano, Akiyo; Tanabe, Soichi; Morita, Hidetoshi.

In: International Journal of Microbiology, 2012.

Research output: Contribution to journalArticle

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