Comparative genome analyses of Mycobacterium avium reveal genomic features of its subspecies and strains that cause progression of pulmonary disease

Kei Ichi Uchiya, Shuta Tomida, Taku Nakagawa, Shoki Asahi, Toshiaki Nikai, Kenji Ogawa

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Pulmonary disease caused by nontuberculous mycobacteria (NTM) is increasing worldwide. Mycobacterium avium is the most clinically significant NTM species in humans and animals, and comprises four subspecies: M. avium subsp. avium (MAA), M. avium subsp. silvaticum (MAS), M. avium subsp. paratuberculosis (MAP), and M. avium subsp. hominissuis (MAH). To improve our understanding of the genetic landscape and diversity of M. avium and its role in disease, we performed a comparative genome analysis of 79 M. avium strains. Our analysis demonstrated that MAH is an open pan-genome species. Phylogenetic analysis based on single nucleotide variants showed that MAH had the highest degree of sequence variability among the subspecies, and MAH strains isolated in Japan and those isolated abroad possessed distinct phylogenetic features. Furthermore, MAP strains, MAS and MAA strains isolated from birds, and many MAH strains that cause the progression of pulmonary disease were grouped in each specific cluster. Comparative genome analysis revealed the presence of genetic elements specific to each lineage, which are thought to be acquired via horizontal gene transfer during the evolutionary process, and identified potential genetic determinants accounting for the pathogenic and host range characteristics of M. avium.

Original languageEnglish
Article number39750
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - Jan 3 2017

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Mycobacterium avium
respiratory tract diseases
genomics
genome
Mycobacterium avium subsp. silvaticum
Mycobacterium avium subsp. avium
Mycobacterium avium subsp. paratuberculosis
Mycobacterium
phylogeny
host range
nucleotides
Japan
birds

ASJC Scopus subject areas

  • General

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Comparative genome analyses of Mycobacterium avium reveal genomic features of its subspecies and strains that cause progression of pulmonary disease. / Uchiya, Kei Ichi; Tomida, Shuta; Nakagawa, Taku; Asahi, Shoki; Nikai, Toshiaki; Ogawa, Kenji.

In: Scientific Reports, Vol. 7, 39750, 03.01.2017.

Research output: Contribution to journalArticle

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