Comparative evaluation of the stability of seven-transmembrane microbial rhodopsins to various physicochemical stimuli

Naoya Honda, Takashi Tsukamoto, Yuki Sudo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Rhodopsins are seven-transmembrane proteins that function as photoreceptors for a variety of biological processes. Their characteristic visible colors make rhodopsins a good model for membrane-embedded proteins. In this study, by utilizing their color changes, we performed comparative studies on the stability of five microbial rhodopsins using the same instruments, procedures and media. As denaturants, we employed four physicochemical stimuli: (i) thermal perturbation, (ii) the water-soluble reagent hydroxylamine, (iii) the detergent sodium dodecyl sulfate, and (iv) the organic solvent ethanol. On the basis of the results, models for stabilization mechanisms in rhodopsins against each stimulus is proposed.

Original languageEnglish
Pages (from-to)6-14
Number of pages9
JournalChemical Physics Letters
Volume682
DOIs
Publication statusPublished - Aug 16 2017

Fingerprint

Microbial Rhodopsins
Rhodopsin
stimuli
proteins
color
photoreceptors
detergents
evaluation
sodium sulfates
Color
reagents
Hydroxylamine
ethyl alcohol
stabilization
membranes
Sodium Dodecyl Sulfate
perturbation
Detergents
Organic solvents
Membrane Proteins

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

Cite this

Comparative evaluation of the stability of seven-transmembrane microbial rhodopsins to various physicochemical stimuli. / Honda, Naoya; Tsukamoto, Takashi; Sudo, Yuki.

In: Chemical Physics Letters, Vol. 682, 16.08.2017, p. 6-14.

Research output: Contribution to journalArticle

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