Comparative anatomy of gastrin-releasing peptide pathways in the trigeminal sensory system of mouse and the asian house musk shrew Suncus murinus

Keiko Takanami, Kaihei Inoue, Hiroki Mukai, Kei Tamura, Takamichi Jogahara, Sen Ichi Oda, Mitsuhiro Kawata, Tatsuya Sakamoto, Hirotaka Sakamoto

Research output: Contribution to journalArticle

Abstract

Gastrin-releasing peptide (GRP) has recently been identified as an itch-signaling molecule in the primary afferents and spinal cord of rodents. However, little information exists on the expression and localization of GRP in the trigeminal somatosensory system other than in rats. We examined the generality of the trigeminal GRP system in mammals using two distinct species, suncus as a model of specialized placental mammals known to have a welldeveloped trigeminal sensory system and mice as a representative small laboratory animal. We first analyzed the gross morphology of the trigeminal somatosensory system in suncus to provide a brainstem atlas on which to map GRP distribution. Immunohistochemical analyses showed that 8% of trigeminal ganglion neurons in suncus and 6% in mice expressed GRP. Expression was restricted to cells with smaller somata. The GRP-containing fibers were densely distributed in the superficial layers of the caudal part of the trigeminal spinal nucleus (Vc) but rare in the rostral parts, both in suncus and mice. Expression of GRP receptor mRNA and protein was also detected in the Vc of suncus. Taken together, these results suggest that the trigeminal GRP system mediating itch sensation is conserved in mammals.

Original languageEnglish
Pages (from-to)181-190
Number of pages10
JournalActa Histochemica et Cytochemica
Volume49
Issue number6
DOIs
Publication statusPublished - 2016

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Comparative Anatomy
Gastrin-Releasing Peptide
Shrews
Mammals
Bombesin Receptors
Spinal Trigeminal Nucleus
Trigeminal Ganglion
Atlases
Laboratory Animals
Carisoprodol
musk
Brain Stem
Neurons
Rats
Rodentia
Spinal Cord
Animals
Messenger RNA
Molecules
Fibers

Keywords

  • Gastrin-releasing peptide (GRP)
  • Gastrin-releasing peptide receptor (GRPR)
  • Itch
  • Suncus
  • Trigeminal somatosensory system

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Physiology
  • Biochemistry
  • Cell Biology

Cite this

Comparative anatomy of gastrin-releasing peptide pathways in the trigeminal sensory system of mouse and the asian house musk shrew Suncus murinus. / Takanami, Keiko; Inoue, Kaihei; Mukai, Hiroki; Tamura, Kei; Jogahara, Takamichi; Oda, Sen Ichi; Kawata, Mitsuhiro; Sakamoto, Tatsuya; Sakamoto, Hirotaka.

In: Acta Histochemica et Cytochemica, Vol. 49, No. 6, 2016, p. 181-190.

Research output: Contribution to journalArticle

Takanami, Keiko ; Inoue, Kaihei ; Mukai, Hiroki ; Tamura, Kei ; Jogahara, Takamichi ; Oda, Sen Ichi ; Kawata, Mitsuhiro ; Sakamoto, Tatsuya ; Sakamoto, Hirotaka. / Comparative anatomy of gastrin-releasing peptide pathways in the trigeminal sensory system of mouse and the asian house musk shrew Suncus murinus. In: Acta Histochemica et Cytochemica. 2016 ; Vol. 49, No. 6. pp. 181-190.
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