Comparative analysis of fruit ripening and associated genes in two kiwifruit cultivars (‘Sanuki Gold’ and ‘Hayward’) at various storage temperatures

Oscar Witere Mitalo, William Olubero Asiche, Yuka Kasahara, Yasuaki Tosa, Sumire Tokiwa, Koichiro Ushijima, Ryohei Nakano, Yasutaka Kubo

Research output: Contribution to journalArticle

Abstract

Kiwifruit exhibit a peculiar ripening pattern, as extensive softening is known to occur in the absence of any detectable ethylene. We previously demonstrated that this softening is regulated by low temperature independent of ethylene. However, there are no reports that provide comparisons of the ripening patterns among different kiwifruit cultivars at various storage temperatures. The purpose of this study was to compare the ripening responses and associated gene expression in ‘Sanuki Gold’ (Actinidia chinensis var. chinensis) and ‘Hayward’ ((Actinidia chinensis var. deliciosa) fruit, two kiwifruit cultivars differing in on–vine maturity dates and postharvest storability, during storage at 5 °C, 10 °C, 15 °C and 22 °C. Fruit softening, soluble solids concentration (SSC) increase and reduction of titratable acidity (TA) occurred in the absence of any detectable ethylene, and treatment with an ethylene inhibitor 1–methylcyclopropene (1–MCP) failed to suppress the changes, suggesting that they were independent of ethylene. ‘Sanuki Gold’ fruit showed a higher sensitivity to low temperature supported by accelerated fruit softening and TA reduction, and induction of several genes such as AcACO3, AcXET2, AcPG, AcEXP1, AcPMEi, AcGA2ox1, AcMADS2, AcNAC5 and AcbZIP2 at 5 °C, 10 °C and 15 °C within 28 d. By contrast, ‘Hayward’ fruit exhibited a lower sensitivity to low temperature as accelerated softening, TA reduction and induction of most ripening–associated genes were recorded only at 5 °C and 10 °C. These differences in sensitivity to low temperature, between ‘Sanuki Gold’ and ‘Hayward’ fruit, would account for the dissimilarities observed in on–vine maturity dates and postharvest storability.

LanguageEnglish
Pages20-28
Number of pages9
JournalPostharvest Biology and Technology
Volume147
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

kiwifruit
Gold
storage temperature
gold
Fruit
ripening
Temperature
fruits
ethylene
cultivars
Actinidia
titratable acidity
Genes
genes
storage quality
vines
temperature
Actinidia chinensis
ethylene inhibitors
Actinidia deliciosa

Keywords

  • Actinidia chinensis var. chinensis
  • Actinidia chinensis var. deliciosa
  • Low temperature
  • On–vine maturity
  • Softening
  • Storability

ASJC Scopus subject areas

  • Food Science
  • Agronomy and Crop Science
  • Horticulture

Cite this

Comparative analysis of fruit ripening and associated genes in two kiwifruit cultivars (‘Sanuki Gold’ and ‘Hayward’) at various storage temperatures. / Mitalo, Oscar Witere; Asiche, William Olubero; Kasahara, Yuka; Tosa, Yasuaki; Tokiwa, Sumire; Ushijima, Koichiro; Nakano, Ryohei; Kubo, Yasutaka.

In: Postharvest Biology and Technology, Vol. 147, 01.01.2019, p. 20-28.

Research output: Contribution to journalArticle

@article{5af03334f85a4a50b78c967e94f2e36b,
title = "Comparative analysis of fruit ripening and associated genes in two kiwifruit cultivars (‘Sanuki Gold’ and ‘Hayward’) at various storage temperatures",
abstract = "Kiwifruit exhibit a peculiar ripening pattern, as extensive softening is known to occur in the absence of any detectable ethylene. We previously demonstrated that this softening is regulated by low temperature independent of ethylene. However, there are no reports that provide comparisons of the ripening patterns among different kiwifruit cultivars at various storage temperatures. The purpose of this study was to compare the ripening responses and associated gene expression in ‘Sanuki Gold’ (Actinidia chinensis var. chinensis) and ‘Hayward’ ((Actinidia chinensis var. deliciosa) fruit, two kiwifruit cultivars differing in on–vine maturity dates and postharvest storability, during storage at 5 °C, 10 °C, 15 °C and 22 °C. Fruit softening, soluble solids concentration (SSC) increase and reduction of titratable acidity (TA) occurred in the absence of any detectable ethylene, and treatment with an ethylene inhibitor 1–methylcyclopropene (1–MCP) failed to suppress the changes, suggesting that they were independent of ethylene. ‘Sanuki Gold’ fruit showed a higher sensitivity to low temperature supported by accelerated fruit softening and TA reduction, and induction of several genes such as AcACO3, AcXET2, AcPG, AcEXP1, AcPMEi, AcGA2ox1, AcMADS2, AcNAC5 and AcbZIP2 at 5 °C, 10 °C and 15 °C within 28 d. By contrast, ‘Hayward’ fruit exhibited a lower sensitivity to low temperature as accelerated softening, TA reduction and induction of most ripening–associated genes were recorded only at 5 °C and 10 °C. These differences in sensitivity to low temperature, between ‘Sanuki Gold’ and ‘Hayward’ fruit, would account for the dissimilarities observed in on–vine maturity dates and postharvest storability.",
keywords = "Actinidia chinensis var. chinensis, Actinidia chinensis var. deliciosa, Low temperature, On–vine maturity, Softening, Storability",
author = "Mitalo, {Oscar Witere} and Asiche, {William Olubero} and Yuka Kasahara and Yasuaki Tosa and Sumire Tokiwa and Koichiro Ushijima and Ryohei Nakano and Yasutaka Kubo",
year = "2019",
month = "1",
day = "1",
doi = "10.1016/j.postharvbio.2018.08.017",
language = "English",
volume = "147",
pages = "20--28",
journal = "Postharvest Biology and Technology",
issn = "0925-5214",
publisher = "Elsevier",

}

TY - JOUR

T1 - Comparative analysis of fruit ripening and associated genes in two kiwifruit cultivars (‘Sanuki Gold’ and ‘Hayward’) at various storage temperatures

AU - Mitalo, Oscar Witere

AU - Asiche, William Olubero

AU - Kasahara, Yuka

AU - Tosa, Yasuaki

AU - Tokiwa, Sumire

AU - Ushijima, Koichiro

AU - Nakano, Ryohei

AU - Kubo, Yasutaka

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Kiwifruit exhibit a peculiar ripening pattern, as extensive softening is known to occur in the absence of any detectable ethylene. We previously demonstrated that this softening is regulated by low temperature independent of ethylene. However, there are no reports that provide comparisons of the ripening patterns among different kiwifruit cultivars at various storage temperatures. The purpose of this study was to compare the ripening responses and associated gene expression in ‘Sanuki Gold’ (Actinidia chinensis var. chinensis) and ‘Hayward’ ((Actinidia chinensis var. deliciosa) fruit, two kiwifruit cultivars differing in on–vine maturity dates and postharvest storability, during storage at 5 °C, 10 °C, 15 °C and 22 °C. Fruit softening, soluble solids concentration (SSC) increase and reduction of titratable acidity (TA) occurred in the absence of any detectable ethylene, and treatment with an ethylene inhibitor 1–methylcyclopropene (1–MCP) failed to suppress the changes, suggesting that they were independent of ethylene. ‘Sanuki Gold’ fruit showed a higher sensitivity to low temperature supported by accelerated fruit softening and TA reduction, and induction of several genes such as AcACO3, AcXET2, AcPG, AcEXP1, AcPMEi, AcGA2ox1, AcMADS2, AcNAC5 and AcbZIP2 at 5 °C, 10 °C and 15 °C within 28 d. By contrast, ‘Hayward’ fruit exhibited a lower sensitivity to low temperature as accelerated softening, TA reduction and induction of most ripening–associated genes were recorded only at 5 °C and 10 °C. These differences in sensitivity to low temperature, between ‘Sanuki Gold’ and ‘Hayward’ fruit, would account for the dissimilarities observed in on–vine maturity dates and postharvest storability.

AB - Kiwifruit exhibit a peculiar ripening pattern, as extensive softening is known to occur in the absence of any detectable ethylene. We previously demonstrated that this softening is regulated by low temperature independent of ethylene. However, there are no reports that provide comparisons of the ripening patterns among different kiwifruit cultivars at various storage temperatures. The purpose of this study was to compare the ripening responses and associated gene expression in ‘Sanuki Gold’ (Actinidia chinensis var. chinensis) and ‘Hayward’ ((Actinidia chinensis var. deliciosa) fruit, two kiwifruit cultivars differing in on–vine maturity dates and postharvest storability, during storage at 5 °C, 10 °C, 15 °C and 22 °C. Fruit softening, soluble solids concentration (SSC) increase and reduction of titratable acidity (TA) occurred in the absence of any detectable ethylene, and treatment with an ethylene inhibitor 1–methylcyclopropene (1–MCP) failed to suppress the changes, suggesting that they were independent of ethylene. ‘Sanuki Gold’ fruit showed a higher sensitivity to low temperature supported by accelerated fruit softening and TA reduction, and induction of several genes such as AcACO3, AcXET2, AcPG, AcEXP1, AcPMEi, AcGA2ox1, AcMADS2, AcNAC5 and AcbZIP2 at 5 °C, 10 °C and 15 °C within 28 d. By contrast, ‘Hayward’ fruit exhibited a lower sensitivity to low temperature as accelerated softening, TA reduction and induction of most ripening–associated genes were recorded only at 5 °C and 10 °C. These differences in sensitivity to low temperature, between ‘Sanuki Gold’ and ‘Hayward’ fruit, would account for the dissimilarities observed in on–vine maturity dates and postharvest storability.

KW - Actinidia chinensis var. chinensis

KW - Actinidia chinensis var. deliciosa

KW - Low temperature

KW - On–vine maturity

KW - Softening

KW - Storability

UR - http://www.scopus.com/inward/record.url?scp=85053379292&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85053379292&partnerID=8YFLogxK

U2 - 10.1016/j.postharvbio.2018.08.017

DO - 10.1016/j.postharvbio.2018.08.017

M3 - Article

VL - 147

SP - 20

EP - 28

JO - Postharvest Biology and Technology

T2 - Postharvest Biology and Technology

JF - Postharvest Biology and Technology

SN - 0925-5214

ER -