Comparative analysis of brain proteins from p53-deficient mice by two- dimensional electrophoresis

Norie Araki, Tadaomi Morimasa, Tomoko Sakai, Hiroshi Tokuoh, Shunji Yunoue, Masaharu Kamo, Kenji Miyazaki, Koji Abe, Hideyuki Saya, Akira Tsugita

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

p53 is a tumor suppressor protein that regulates many cellular processes including the cell cycle, DNA repair, and apoptosis. It also serves as a critical regulator of neuronal apoptosis in the central nervous system (CNS). To elucidate the role of p53 in the CNS, brain proteins of p53 knock-out mice (p53-/-) were analyzed by two-dimensional gel electrophoresis (2-DE) and compared with those from p53 wild type (p53+/+) mice. Six types of brain tissue (temporal cortex, cerebellum, hippocampus, striatum, olfactory bulb, and cervical spinal cord) and other control tissues (lung and blood) from 18- week-old non-stress-induced mice were analyzed. The morphology of brains from p53-/- mice appeared to be normal and identical to that of p53+/+ mice, although lungs showed diffuse tumors that may have been caused by p53 deficiency. Comparative 2-D gel analysis showed that, on average, 7 of 886 spots from brain tissue were p53-/- specific, whereas 12 of 1008 spots from lung tissue were p53-/- specific. N-terminal amino acid sequence was determined for p53-/- specific proteins. In all brain tissues from p53-/- mice, a newly identified mouse mitochondrial NADH-ubiquinone oxidoreductase 24 kDa subunit showed decreased expression, and apolipoprotein A1 acidic forms showed increased expression. In addition, brain-type creatine kinase B chain and tubulin β-5 N-terminal fragment were increased in the p53-/- cerebellum, and a new protein in mouse, hydroxyacylglutathione hydrolase (glyoxalase II) was decreased in the temporal cortex of p53-/- mice. The alterations in protein expression identified in this study may imply a p53- related brain function. This is the first proteomic analysis on the p53-/- mouse brain, and further information based on this study will provide new insights into the p53 function in the CNS.

Original languageEnglish
Pages (from-to)1880-1889
Number of pages10
JournalElectrophoresis
Volume21
Issue number9
DOIs
Publication statusPublished - 2000

Fingerprint

Electrophoresis
Brain
hydroxyacylglutathione hydrolase
Tissue
Neurology
Proteins
Central Nervous System
Temporal Lobe
Gels
BB Form Creatine Kinase
Lung
Cerebellum
Apoptosis
Electron Transport Complex I
Tumor Suppressor Proteins
Apolipoprotein A-I
Tubulin
Olfactory Bulb
Tumors
Electrophoresis, Gel, Two-Dimensional

Keywords

  • Brain
  • N-terminal amino acid sequence
  • p53 gene knock-out mice
  • p53 tumor suppressor protein
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Comparative analysis of brain proteins from p53-deficient mice by two- dimensional electrophoresis. / Araki, Norie; Morimasa, Tadaomi; Sakai, Tomoko; Tokuoh, Hiroshi; Yunoue, Shunji; Kamo, Masaharu; Miyazaki, Kenji; Abe, Koji; Saya, Hideyuki; Tsugita, Akira.

In: Electrophoresis, Vol. 21, No. 9, 2000, p. 1880-1889.

Research output: Contribution to journalArticle

Araki, N, Morimasa, T, Sakai, T, Tokuoh, H, Yunoue, S, Kamo, M, Miyazaki, K, Abe, K, Saya, H & Tsugita, A 2000, 'Comparative analysis of brain proteins from p53-deficient mice by two- dimensional electrophoresis', Electrophoresis, vol. 21, no. 9, pp. 1880-1889. https://doi.org/10.1002/(SICI)1522-2683(20000501)21:9<1880::AID-ELPS1880>3.0.CO;2-9
Araki, Norie ; Morimasa, Tadaomi ; Sakai, Tomoko ; Tokuoh, Hiroshi ; Yunoue, Shunji ; Kamo, Masaharu ; Miyazaki, Kenji ; Abe, Koji ; Saya, Hideyuki ; Tsugita, Akira. / Comparative analysis of brain proteins from p53-deficient mice by two- dimensional electrophoresis. In: Electrophoresis. 2000 ; Vol. 21, No. 9. pp. 1880-1889.
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