Comparative analysis of basal lamina type IV collagen α chains, matrix metalloproteinases-2 and -9 expressions in oral dysplasia and invasive carcinoma

Ryo Tamamura, Hitoshi Nagatsuka, Chong Huat Siar, Naoki Katase, Ichiro Naito, Yoshikazu Sado, Noriyuki Nagai

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The aim of this study was to compare the expressions of basal lamina (BL) collagen IV α chains and matrix metalloproteinases (MMP)-2 and MMP-9 in oral dysplasia (OED) and invasive carcinoma. Ten cases each of OEDs, carcinomas-in situ and oral squamous cell carcinomas (OSCCs) were examined by immunohistochemistry. Another 5 cases, each of normal and hyperplastic oral mucosa, served as controls. Results showed that α1(IV)/α2(IV) and α5(IV)/α6(IV) chains were intact in BLs of control and OEDs. In BLs of carcinoma-in situ, α1(IV)/α2(IV) chains preceded α5(IV)/α6(IV) chains in showing incipient signs of disruption. OSCCs exhibited varying degrees of collagen α(IV) chain degradation. MMP-2 and MMP-9 were absent in controls and OED, but weakly detectable in carcinoma-in situ. In OSCC, these proteolytic enzymes were expressed in areas corresponding to collagen α(IV) chain loss. Enzymatic activity was enhanced in higher grade OSCC, and along the tumor advancing front. Overall the present findings suggest that loss of BL collagen α(IV) chains coincided with gain of expression for MMP-2 and MMP-9, and that these protein alterations are crucial events during progression from OED to OSCC.

Original languageEnglish
Pages (from-to)113-119
Number of pages7
JournalActa Histochemica
Volume115
Issue number2
DOIs
Publication statusPublished - Mar 2013

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Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Basement Membrane
Squamous Cell Carcinoma
Collagen
Carcinoma
Carcinoma in Situ
Mouth Mucosa
Peptide Hydrolases
Immunohistochemistry
Spinal Cord Dorsal Horn
Neoplasms
Proteins

Keywords

  • Basal lamina
  • Carcinoma-in situ
  • Collagen IV α chains
  • Epithelial dysplasia
  • Human
  • MMP-2 and -9
  • Oral squamous cell carcinoma

ASJC Scopus subject areas

  • Cell Biology
  • Histology

Cite this

Comparative analysis of basal lamina type IV collagen α chains, matrix metalloproteinases-2 and -9 expressions in oral dysplasia and invasive carcinoma. / Tamamura, Ryo; Nagatsuka, Hitoshi; Siar, Chong Huat; Katase, Naoki; Naito, Ichiro; Sado, Yoshikazu; Nagai, Noriyuki.

In: Acta Histochemica, Vol. 115, No. 2, 03.2013, p. 113-119.

Research output: Contribution to journalArticle

Tamamura, Ryo ; Nagatsuka, Hitoshi ; Siar, Chong Huat ; Katase, Naoki ; Naito, Ichiro ; Sado, Yoshikazu ; Nagai, Noriyuki. / Comparative analysis of basal lamina type IV collagen α chains, matrix metalloproteinases-2 and -9 expressions in oral dysplasia and invasive carcinoma. In: Acta Histochemica. 2013 ; Vol. 115, No. 2. pp. 113-119.
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