Abstract
Background: The chondrosarcoma-derived HCS-2/8 has been known to be an excellent model of human articular chondrocytes. By mimicking the arthritic conditions through the treatment of HCS-2/8 cells with cytokines, we estimated the gene expression response of ccn1 and ccn2 during the course of joint inflammation in vitro. Results: In order to mimic the initiation of inflammation, HCS-2/8 cells were treated with tumor necrosis factor (TNF)-α. To induce pro-inflammatory or reparative responses, TGF-β was employed. Effects of an anti-inflammatory glucocorticoid were also evaluated. After stimulation, expression levels of ccn1 and ccn2 were quantitatively analyzed. Surprisingly, not only ccn2, but also ccn1 expression was repressed upon TNF-α stimulation, whereas both mRNAs were uniformly induced by transforming growth factor (TGF)-β and a glucocorticoid. Conclusion: These results describing the same response during the course of inflammation suggest similar and co-operative roles of these 2 ccn family members in the course of arthritis.
| Original language | English |
|---|---|
| Article number | 6 |
| Journal | Cell Communication and Signaling |
| Volume | 3 |
| DOIs | |
| Publication status | Published - Apr 15 2005 |
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ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
- Biochemistry
Cite this
Comparable response of ccn1 with ccn2 genes upon arthritis : An in vitro evaluation with a human chondrocytic cell line stimulated by a set of cytokines. / Moritani, Norifumi; Kubota, Satoshi; Sugahara, Toshio; Takigawa, Masaharu.
In: Cell Communication and Signaling, Vol. 3, 6, 15.04.2005.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparable response of ccn1 with ccn2 genes upon arthritis
T2 - An in vitro evaluation with a human chondrocytic cell line stimulated by a set of cytokines
AU - Moritani, Norifumi
AU - Kubota, Satoshi
AU - Sugahara, Toshio
AU - Takigawa, Masaharu
PY - 2005/4/15
Y1 - 2005/4/15
N2 - Background: The chondrosarcoma-derived HCS-2/8 has been known to be an excellent model of human articular chondrocytes. By mimicking the arthritic conditions through the treatment of HCS-2/8 cells with cytokines, we estimated the gene expression response of ccn1 and ccn2 during the course of joint inflammation in vitro. Results: In order to mimic the initiation of inflammation, HCS-2/8 cells were treated with tumor necrosis factor (TNF)-α. To induce pro-inflammatory or reparative responses, TGF-β was employed. Effects of an anti-inflammatory glucocorticoid were also evaluated. After stimulation, expression levels of ccn1 and ccn2 were quantitatively analyzed. Surprisingly, not only ccn2, but also ccn1 expression was repressed upon TNF-α stimulation, whereas both mRNAs were uniformly induced by transforming growth factor (TGF)-β and a glucocorticoid. Conclusion: These results describing the same response during the course of inflammation suggest similar and co-operative roles of these 2 ccn family members in the course of arthritis.
AB - Background: The chondrosarcoma-derived HCS-2/8 has been known to be an excellent model of human articular chondrocytes. By mimicking the arthritic conditions through the treatment of HCS-2/8 cells with cytokines, we estimated the gene expression response of ccn1 and ccn2 during the course of joint inflammation in vitro. Results: In order to mimic the initiation of inflammation, HCS-2/8 cells were treated with tumor necrosis factor (TNF)-α. To induce pro-inflammatory or reparative responses, TGF-β was employed. Effects of an anti-inflammatory glucocorticoid were also evaluated. After stimulation, expression levels of ccn1 and ccn2 were quantitatively analyzed. Surprisingly, not only ccn2, but also ccn1 expression was repressed upon TNF-α stimulation, whereas both mRNAs were uniformly induced by transforming growth factor (TGF)-β and a glucocorticoid. Conclusion: These results describing the same response during the course of inflammation suggest similar and co-operative roles of these 2 ccn family members in the course of arthritis.
UR - http://www.scopus.com/inward/record.url?scp=33750169244&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33750169244&partnerID=8YFLogxK
U2 - 10.1186/1478-811X-3-6
DO - 10.1186/1478-811X-3-6
M3 - Article
AN - SCOPUS:33750169244
VL - 3
JO - Cell Communication and Signaling
JF - Cell Communication and Signaling
SN - 1478-811X
M1 - 6
ER -