Common clonal origin of lymphocytes and plasma cells in splenic lymphoma with villous lymphocytes

Yoshio Katayama, Kensuke Kojima, Tadashi Yoshino, Yoshinobu Matsuo, Masafumi Isokawa, Tomofumi Yano, Hideyaki Oka, Mika Yamaguchi, Seigo Deguchi, Junjiro Tsuchiyama, Kyoichi Hayashi, Takanori Teshima, Katsuji Shinagawa, Fumihiko Ishimaru, Eijiro Omoto, Mine Harada

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In two-thirds of patients with splenic lymphoma with villous lymphocytes (SLVL) a small amount of M-protein can be detected in association with the presence of plasma cells in the peripheral blood (PB) and/or bone marrow (BM). However, it is not known whether lymphoma cells trod plasma cells originate from the same clone. In this report we describe a case of SLVL which was characterized by the presence of marked monoclonal gammopathy (IgG- κ 90 g/l) and increased plasma cells in the BM. In an attempt to elucidate the origin of lymphoma cells and plasma cells, we performed morphological, cytogenetic and molecular studies on PB mononuclear cells (PBMNC) without plasma cells and BMMNC containing 10% plasma cells from this patient. Immunofluorescence showed that lymphoma cells and plasma cells were positive for cytoplasmic γ heavy and κ light chains. Well-developed endoplasmic reticulum was observed in the cytoplasmic organelles of PBMNC using an electron microscope. The mean IgG concentration in the 3 d supernatant cultures of PBMNC was 374 ± 24 μg/l. More than 50% PBMNC differentiated into plasmacytoid cells in 6 d of liquid culture with IL-3 and IL-6. Analysis by two colour FISH revealed that karyotypic abnormalities of monosomy X and trisomy 17 existed simultaneously in both lymphoma cells and plasma cells, JH gene rearranged bands from PBMNC and BMMNC by Southern blot hybridization were identical, whereas DNAs from PBMNC failed to hybridize with the Cμ probe. These observations strongly suggest that lymphoma cells and plasma cells originate from the same clone, and that plasma cells, as well as lymphoma cells, which have undergone class switch recombination, could produce IgG type M-protein in this case.

Original languageEnglish
Pages (from-to)626-634
Number of pages9
JournalBritish Journal of Haematology
Volume97
Issue number3
Publication statusPublished - 1997

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Plasma Cells
Lymphoma
Lymphocytes
Immunoglobulin G
Clone Cells
Bone Marrow
Paraproteinemias
Turner Syndrome
Interleukin-3
Southern Blotting
Cytogenetics
Endoplasmic Reticulum
Organelles
Genetic Recombination
Fluorescent Antibody Technique
Interleukin-6
Blood Cells
Proteins
Color
Electrons

Keywords

  • common clonal origin
  • immunoglobulin producing cells
  • lymphocytes and plasma cells
  • plasma cell differentiation
  • SLVL

ASJC Scopus subject areas

  • Hematology

Cite this

Katayama, Y., Kojima, K., Yoshino, T., Matsuo, Y., Isokawa, M., Yano, T., ... Harada, M. (1997). Common clonal origin of lymphocytes and plasma cells in splenic lymphoma with villous lymphocytes. British Journal of Haematology, 97(3), 626-634.

Common clonal origin of lymphocytes and plasma cells in splenic lymphoma with villous lymphocytes. / Katayama, Yoshio; Kojima, Kensuke; Yoshino, Tadashi; Matsuo, Yoshinobu; Isokawa, Masafumi; Yano, Tomofumi; Oka, Hideyaki; Yamaguchi, Mika; Deguchi, Seigo; Tsuchiyama, Junjiro; Hayashi, Kyoichi; Teshima, Takanori; Shinagawa, Katsuji; Ishimaru, Fumihiko; Omoto, Eijiro; Harada, Mine.

In: British Journal of Haematology, Vol. 97, No. 3, 1997, p. 626-634.

Research output: Contribution to journalArticle

Katayama, Y, Kojima, K, Yoshino, T, Matsuo, Y, Isokawa, M, Yano, T, Oka, H, Yamaguchi, M, Deguchi, S, Tsuchiyama, J, Hayashi, K, Teshima, T, Shinagawa, K, Ishimaru, F, Omoto, E & Harada, M 1997, 'Common clonal origin of lymphocytes and plasma cells in splenic lymphoma with villous lymphocytes', British Journal of Haematology, vol. 97, no. 3, pp. 626-634.
Katayama, Yoshio ; Kojima, Kensuke ; Yoshino, Tadashi ; Matsuo, Yoshinobu ; Isokawa, Masafumi ; Yano, Tomofumi ; Oka, Hideyaki ; Yamaguchi, Mika ; Deguchi, Seigo ; Tsuchiyama, Junjiro ; Hayashi, Kyoichi ; Teshima, Takanori ; Shinagawa, Katsuji ; Ishimaru, Fumihiko ; Omoto, Eijiro ; Harada, Mine. / Common clonal origin of lymphocytes and plasma cells in splenic lymphoma with villous lymphocytes. In: British Journal of Haematology. 1997 ; Vol. 97, No. 3. pp. 626-634.
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abstract = "In two-thirds of patients with splenic lymphoma with villous lymphocytes (SLVL) a small amount of M-protein can be detected in association with the presence of plasma cells in the peripheral blood (PB) and/or bone marrow (BM). However, it is not known whether lymphoma cells trod plasma cells originate from the same clone. In this report we describe a case of SLVL which was characterized by the presence of marked monoclonal gammopathy (IgG- κ 90 g/l) and increased plasma cells in the BM. In an attempt to elucidate the origin of lymphoma cells and plasma cells, we performed morphological, cytogenetic and molecular studies on PB mononuclear cells (PBMNC) without plasma cells and BMMNC containing 10{\%} plasma cells from this patient. Immunofluorescence showed that lymphoma cells and plasma cells were positive for cytoplasmic γ heavy and κ light chains. Well-developed endoplasmic reticulum was observed in the cytoplasmic organelles of PBMNC using an electron microscope. The mean IgG concentration in the 3 d supernatant cultures of PBMNC was 374 ± 24 μg/l. More than 50{\%} PBMNC differentiated into plasmacytoid cells in 6 d of liquid culture with IL-3 and IL-6. Analysis by two colour FISH revealed that karyotypic abnormalities of monosomy X and trisomy 17 existed simultaneously in both lymphoma cells and plasma cells, JH gene rearranged bands from PBMNC and BMMNC by Southern blot hybridization were identical, whereas DNAs from PBMNC failed to hybridize with the Cμ probe. These observations strongly suggest that lymphoma cells and plasma cells originate from the same clone, and that plasma cells, as well as lymphoma cells, which have undergone class switch recombination, could produce IgG type M-protein in this case.",
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AU - Katayama, Yoshio

AU - Kojima, Kensuke

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AU - Isokawa, Masafumi

AU - Yano, Tomofumi

AU - Oka, Hideyaki

AU - Yamaguchi, Mika

AU - Deguchi, Seigo

AU - Tsuchiyama, Junjiro

AU - Hayashi, Kyoichi

AU - Teshima, Takanori

AU - Shinagawa, Katsuji

AU - Ishimaru, Fumihiko

AU - Omoto, Eijiro

AU - Harada, Mine

PY - 1997

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N2 - In two-thirds of patients with splenic lymphoma with villous lymphocytes (SLVL) a small amount of M-protein can be detected in association with the presence of plasma cells in the peripheral blood (PB) and/or bone marrow (BM). However, it is not known whether lymphoma cells trod plasma cells originate from the same clone. In this report we describe a case of SLVL which was characterized by the presence of marked monoclonal gammopathy (IgG- κ 90 g/l) and increased plasma cells in the BM. In an attempt to elucidate the origin of lymphoma cells and plasma cells, we performed morphological, cytogenetic and molecular studies on PB mononuclear cells (PBMNC) without plasma cells and BMMNC containing 10% plasma cells from this patient. Immunofluorescence showed that lymphoma cells and plasma cells were positive for cytoplasmic γ heavy and κ light chains. Well-developed endoplasmic reticulum was observed in the cytoplasmic organelles of PBMNC using an electron microscope. The mean IgG concentration in the 3 d supernatant cultures of PBMNC was 374 ± 24 μg/l. More than 50% PBMNC differentiated into plasmacytoid cells in 6 d of liquid culture with IL-3 and IL-6. Analysis by two colour FISH revealed that karyotypic abnormalities of monosomy X and trisomy 17 existed simultaneously in both lymphoma cells and plasma cells, JH gene rearranged bands from PBMNC and BMMNC by Southern blot hybridization were identical, whereas DNAs from PBMNC failed to hybridize with the Cμ probe. These observations strongly suggest that lymphoma cells and plasma cells originate from the same clone, and that plasma cells, as well as lymphoma cells, which have undergone class switch recombination, could produce IgG type M-protein in this case.

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