Abstract
In two-thirds of patients with splenic lymphoma with villous lymphocytes (SLVL) a small amount of M-protein can be detected in association with the presence of plasma cells in the peripheral blood (PB) and/or bone marrow (BM). However, it is not known whether lymphoma cells trod plasma cells originate from the same clone. In this report we describe a case of SLVL which was characterized by the presence of marked monoclonal gammopathy (IgG- κ 90 g/l) and increased plasma cells in the BM. In an attempt to elucidate the origin of lymphoma cells and plasma cells, we performed morphological, cytogenetic and molecular studies on PB mononuclear cells (PBMNC) without plasma cells and BMMNC containing 10% plasma cells from this patient. Immunofluorescence showed that lymphoma cells and plasma cells were positive for cytoplasmic γ heavy and κ light chains. Well-developed endoplasmic reticulum was observed in the cytoplasmic organelles of PBMNC using an electron microscope. The mean IgG concentration in the 3 d supernatant cultures of PBMNC was 374 ± 24 μg/l. More than 50% PBMNC differentiated into plasmacytoid cells in 6 d of liquid culture with IL-3 and IL-6. Analysis by two colour FISH revealed that karyotypic abnormalities of monosomy X and trisomy 17 existed simultaneously in both lymphoma cells and plasma cells, JH gene rearranged bands from PBMNC and BMMNC by Southern blot hybridization were identical, whereas DNAs from PBMNC failed to hybridize with the Cμ probe. These observations strongly suggest that lymphoma cells and plasma cells originate from the same clone, and that plasma cells, as well as lymphoma cells, which have undergone class switch recombination, could produce IgG type M-protein in this case.
| Original language | English |
|---|---|
| Pages (from-to) | 626-634 |
| Number of pages | 9 |
| Journal | British Journal of Haematology |
| Volume | 97 |
| Issue number | 3 |
| Publication status | Published - 1997 |
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Keywords
- common clonal origin
- immunoglobulin producing cells
- lymphocytes and plasma cells
- plasma cell differentiation
- SLVL
ASJC Scopus subject areas
- Hematology
Cite this
Common clonal origin of lymphocytes and plasma cells in splenic lymphoma with villous lymphocytes. / Katayama, Yoshio; Kojima, Kensuke; Yoshino, Tadashi; Matsuo, Yoshinobu; Isokawa, Masafumi; Yano, Tomofumi; Oka, Hideyaki; Yamaguchi, Mika; Deguchi, Seigo; Tsuchiyama, Junjiro; Hayashi, Kyoichi; Teshima, Takanori; Shinagawa, Katsuji; Ishimaru, Fumihiko; Omoto, Eijiro; Harada, Mine.
In: British Journal of Haematology, Vol. 97, No. 3, 1997, p. 626-634.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Common clonal origin of lymphocytes and plasma cells in splenic lymphoma with villous lymphocytes
AU - Katayama, Yoshio
AU - Kojima, Kensuke
AU - Yoshino, Tadashi
AU - Matsuo, Yoshinobu
AU - Isokawa, Masafumi
AU - Yano, Tomofumi
AU - Oka, Hideyaki
AU - Yamaguchi, Mika
AU - Deguchi, Seigo
AU - Tsuchiyama, Junjiro
AU - Hayashi, Kyoichi
AU - Teshima, Takanori
AU - Shinagawa, Katsuji
AU - Ishimaru, Fumihiko
AU - Omoto, Eijiro
AU - Harada, Mine
PY - 1997
Y1 - 1997
N2 - In two-thirds of patients with splenic lymphoma with villous lymphocytes (SLVL) a small amount of M-protein can be detected in association with the presence of plasma cells in the peripheral blood (PB) and/or bone marrow (BM). However, it is not known whether lymphoma cells trod plasma cells originate from the same clone. In this report we describe a case of SLVL which was characterized by the presence of marked monoclonal gammopathy (IgG- κ 90 g/l) and increased plasma cells in the BM. In an attempt to elucidate the origin of lymphoma cells and plasma cells, we performed morphological, cytogenetic and molecular studies on PB mononuclear cells (PBMNC) without plasma cells and BMMNC containing 10% plasma cells from this patient. Immunofluorescence showed that lymphoma cells and plasma cells were positive for cytoplasmic γ heavy and κ light chains. Well-developed endoplasmic reticulum was observed in the cytoplasmic organelles of PBMNC using an electron microscope. The mean IgG concentration in the 3 d supernatant cultures of PBMNC was 374 ± 24 μg/l. More than 50% PBMNC differentiated into plasmacytoid cells in 6 d of liquid culture with IL-3 and IL-6. Analysis by two colour FISH revealed that karyotypic abnormalities of monosomy X and trisomy 17 existed simultaneously in both lymphoma cells and plasma cells, JH gene rearranged bands from PBMNC and BMMNC by Southern blot hybridization were identical, whereas DNAs from PBMNC failed to hybridize with the Cμ probe. These observations strongly suggest that lymphoma cells and plasma cells originate from the same clone, and that plasma cells, as well as lymphoma cells, which have undergone class switch recombination, could produce IgG type M-protein in this case.
AB - In two-thirds of patients with splenic lymphoma with villous lymphocytes (SLVL) a small amount of M-protein can be detected in association with the presence of plasma cells in the peripheral blood (PB) and/or bone marrow (BM). However, it is not known whether lymphoma cells trod plasma cells originate from the same clone. In this report we describe a case of SLVL which was characterized by the presence of marked monoclonal gammopathy (IgG- κ 90 g/l) and increased plasma cells in the BM. In an attempt to elucidate the origin of lymphoma cells and plasma cells, we performed morphological, cytogenetic and molecular studies on PB mononuclear cells (PBMNC) without plasma cells and BMMNC containing 10% plasma cells from this patient. Immunofluorescence showed that lymphoma cells and plasma cells were positive for cytoplasmic γ heavy and κ light chains. Well-developed endoplasmic reticulum was observed in the cytoplasmic organelles of PBMNC using an electron microscope. The mean IgG concentration in the 3 d supernatant cultures of PBMNC was 374 ± 24 μg/l. More than 50% PBMNC differentiated into plasmacytoid cells in 6 d of liquid culture with IL-3 and IL-6. Analysis by two colour FISH revealed that karyotypic abnormalities of monosomy X and trisomy 17 existed simultaneously in both lymphoma cells and plasma cells, JH gene rearranged bands from PBMNC and BMMNC by Southern blot hybridization were identical, whereas DNAs from PBMNC failed to hybridize with the Cμ probe. These observations strongly suggest that lymphoma cells and plasma cells originate from the same clone, and that plasma cells, as well as lymphoma cells, which have undergone class switch recombination, could produce IgG type M-protein in this case.
KW - common clonal origin
KW - immunoglobulin producing cells
KW - lymphocytes and plasma cells
KW - plasma cell differentiation
KW - SLVL
UR - http://www.scopus.com/inward/record.url?scp=8244239684&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=8244239684&partnerID=8YFLogxK
M3 - Article
C2 - 9207411
AN - SCOPUS:8244239684
VL - 97
SP - 626
EP - 634
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 3
ER -