Combined use of bFGF and GDF-5 enhances the healing of medial collateral ligament injury

Kenta Saiga; Takayuki Furumatsu; Aki Yoshida; Shin Masuda; Shota Takihira; Nobuhiro Abe; Toshifumi Ozaki

doi:10.1016/j.bbrc.2010.10.026

Combined use of bFGF and GDF-5 enhances the healing of medial collateral ligament injury

Kenta Saiga, Takayuki Furumatsu, Aki Yoshida, Shin Masuda, Shota Takihira, Nobuhiro Abe, Toshifumi Ozaki

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Basic fibroblast growth factor (bFGF) and growth and differentiation factor (GDF)-5 stimulate the healing of medial collateral ligament (MCL) injury. However, the effect of isolated and combined use of bFGF/GDF-5 remains still unclear. We investigated cellular proliferation and migration responding to bFGF/GDF-5 using rabbit MCL fibroblasts. Rabbit MCL injury was treated by bFGF and/or GDF-5 with peptide hydrogels. Gene expression and deposition of collagens in healing tissues were evaluated. bFGF/GDF-5 treatment additively enhanced cell proliferation and migration. bFGF/GDF-5 hydrogels stimulated Col1a1 expression without increasing Col3a1 expression. Combined use of bFGF/GDF-5 stimulated type I collagen deposition and the reorganization of fiber alignment, and induced better morphology of fibroblasts in healing MCLs. Our study indicates that combined use of bFGF/GDF-5 might enhance MCL healing by increasing proliferation and migration of MCL fibroblasts, and by regulating collagen synthesis and connective fiber alignment.

Original language	English
Pages (from-to)	329-334
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	402
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2010.10.026
Publication status	Published - Nov 12 2010

Keywords

BFGF
GDF-5
Hydrogel scaffold
Ligament healing
Medial collateral ligament

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Combined use of bFGF and GDF-5 enhances the healing of medial collateral ligament injury",

abstract = "Basic fibroblast growth factor (bFGF) and growth and differentiation factor (GDF)-5 stimulate the healing of medial collateral ligament (MCL) injury. However, the effect of isolated and combined use of bFGF/GDF-5 remains still unclear. We investigated cellular proliferation and migration responding to bFGF/GDF-5 using rabbit MCL fibroblasts. Rabbit MCL injury was treated by bFGF and/or GDF-5 with peptide hydrogels. Gene expression and deposition of collagens in healing tissues were evaluated. bFGF/GDF-5 treatment additively enhanced cell proliferation and migration. bFGF/GDF-5 hydrogels stimulated Col1a1 expression without increasing Col3a1 expression. Combined use of bFGF/GDF-5 stimulated type I collagen deposition and the reorganization of fiber alignment, and induced better morphology of fibroblasts in healing MCLs. Our study indicates that combined use of bFGF/GDF-5 might enhance MCL healing by increasing proliferation and migration of MCL fibroblasts, and by regulating collagen synthesis and connective fiber alignment.",

keywords = "BFGF, GDF-5, Hydrogel scaffold, Ligament healing, Medial collateral ligament",

author = "Kenta Saiga and Takayuki Furumatsu and Aki Yoshida and Shin Masuda and Shota Takihira and Nobuhiro Abe and Toshifumi Ozaki",

note = "Funding Information: We are grateful to Ms. Motomi Hachioji and our colleagues at the Department of Orthopaedic Surgery for their continuing support. This work was supported by Japan Society for the Promotion of Science (No. 20791040 ), the Japanese Foundation for Research and Promotion of Endoscopy, Ryobi Teien Memory Foundation, Okayama Medical Foundation, and Child Health and Development from the Ministry of Health, Labor and Welfare .",

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AU - Saiga, Kenta

AU - Furumatsu, Takayuki

AU - Yoshida, Aki

AU - Masuda, Shin

AU - Takihira, Shota

AU - Abe, Nobuhiro

AU - Ozaki, Toshifumi

N1 - Funding Information: We are grateful to Ms. Motomi Hachioji and our colleagues at the Department of Orthopaedic Surgery for their continuing support. This work was supported by Japan Society for the Promotion of Science (No. 20791040 ), the Japanese Foundation for Research and Promotion of Endoscopy, Ryobi Teien Memory Foundation, Okayama Medical Foundation, and Child Health and Development from the Ministry of Health, Labor and Welfare .

PY - 2010/11/12

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N2 - Basic fibroblast growth factor (bFGF) and growth and differentiation factor (GDF)-5 stimulate the healing of medial collateral ligament (MCL) injury. However, the effect of isolated and combined use of bFGF/GDF-5 remains still unclear. We investigated cellular proliferation and migration responding to bFGF/GDF-5 using rabbit MCL fibroblasts. Rabbit MCL injury was treated by bFGF and/or GDF-5 with peptide hydrogels. Gene expression and deposition of collagens in healing tissues were evaluated. bFGF/GDF-5 treatment additively enhanced cell proliferation and migration. bFGF/GDF-5 hydrogels stimulated Col1a1 expression without increasing Col3a1 expression. Combined use of bFGF/GDF-5 stimulated type I collagen deposition and the reorganization of fiber alignment, and induced better morphology of fibroblasts in healing MCLs. Our study indicates that combined use of bFGF/GDF-5 might enhance MCL healing by increasing proliferation and migration of MCL fibroblasts, and by regulating collagen synthesis and connective fiber alignment.

AB - Basic fibroblast growth factor (bFGF) and growth and differentiation factor (GDF)-5 stimulate the healing of medial collateral ligament (MCL) injury. However, the effect of isolated and combined use of bFGF/GDF-5 remains still unclear. We investigated cellular proliferation and migration responding to bFGF/GDF-5 using rabbit MCL fibroblasts. Rabbit MCL injury was treated by bFGF and/or GDF-5 with peptide hydrogels. Gene expression and deposition of collagens in healing tissues were evaluated. bFGF/GDF-5 treatment additively enhanced cell proliferation and migration. bFGF/GDF-5 hydrogels stimulated Col1a1 expression without increasing Col3a1 expression. Combined use of bFGF/GDF-5 stimulated type I collagen deposition and the reorganization of fiber alignment, and induced better morphology of fibroblasts in healing MCLs. Our study indicates that combined use of bFGF/GDF-5 might enhance MCL healing by increasing proliferation and migration of MCL fibroblasts, and by regulating collagen synthesis and connective fiber alignment.

KW - BFGF

KW - GDF-5

KW - Hydrogel scaffold

KW - Ligament healing

KW - Medial collateral ligament

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Combined use of bFGF and GDF-5 enhances the healing of medial collateral ligament injury

Abstract

Keywords

ASJC Scopus subject areas

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