Combined radiation and gene therapy for brain tumors with adenovirus-mediated transfer of cytosine deaminase and uracil phosphoribosyltransferase genes

Hirokazu Kambara, Takashi Tamiya, Yasuhiro Ono, Shinji Ohtsuka, Kinya Terada, Yoshiaki Adachi, Tomotsugu Ichikawa, Hirofumi Hamada, Takashi Ohmoto

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Radiation therapy is an established modality for the treatment of malignant gliomas. Several reports have shown the advantage of additional radiation in combination with gene therapy. In this study, we investigated the ability of radiation therapy to enhance 5-fluorocytosine (5-FC)/cytosine deaminase (CD) plus uracil phosphoribosyltransferase (UPRT) gene therapy in malignant gliomas. In vitro study suggested evidence of a significant cytotoxic interaction between radiation therapy and 5-FC/CD+UPRT gene therapy for glioma cells. In vivo experiments demonstrated that the combination of gene therapy and radiation possessed superior antitumor effect in comparison to single therapy. However, the adverse effects of radiation therapy in combination with the gene therapy were observed with respect to normal brain. This combination therapy may be feasible for the treatment of gliomas, although the radiation dose and area should be reduced in order to prevent side effects.

Original languageEnglish
Pages (from-to)840-845
Number of pages6
JournalCancer Gene Therapy
Volume9
Issue number10
DOIs
Publication statusPublished - 2002

Keywords

  • Adenovirus vector
  • Cytosine deaminase
  • Gene therapy
  • Glioma
  • Radiation
  • Uracil phosphoribosyltransferase

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Cancer Research

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