Combined cuff and suture technique for orthotopic whole intestinal transplantation in rats

Atsunori Nakao, Kazunori Tahara, Seiichiro Inoue, Kouichi Mizuta, Takayuki Takeichi, Hiroo Uchida, Noriaki Tanaka, Eiji Kobayashi

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


For the purpose of immunological study on small intestinal transplantation (SIT), rat SIT models using direct suture technique widely have been used, which requires at least several months of training for microsurgery. Alternatively, a simple cuff technique for SIT has been mainly used by us, which reduces warm-ischemic time and the training period, but the entire intestinal grafts usually obtain a limited blood supply. This report describes a modification of a combined cuff and suture technique for rat SIT to aid beginning microsurgical transplantation researchers. The advantages are 1) use of only arterial suturing, making it easier for beginners, with the cuff technique applied to the more difficult vein anastomosis; 2) achievement of better arterial inflow and graft survival than when the arterial cuff technique is used; and 3) doing only partial clamping of the aorta, which improves animal survival and success of the procedure, A very high successful rate in orthotopic whole SIT was achieved even by beginners.

Original languageEnglish
Pages (from-to)85-90
Number of pages6
Issue number3
Publication statusPublished - Jan 1 2002
Externally publishedYes

ASJC Scopus subject areas

  • Surgery


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