Combined apoptotic effects of peptide and miRNA in a peptide/miRNA nanocomplex

Hyungjin Kim, Mizuki Kitamatsu, Takashi Ohtsuki

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The present study investigated combined biological effects of peptide and miRNA in a peptide/miRNA nanocomplex. We utilized TatBim peptide as a cell-penetrating peptide-based RNA carrier with apoptotic activity. miRNA with apoptotic activity (miR-34a) was used for complex formation to investigate the additional effects of the combination with TatBim peptide. TatBim peptide and the miRNA formed nanocomplexes (approximately 250 nm in diameter), and these complexes were efficiently internalized by cells. Despite its efficient cell internalization, apoptotic activity of the nanocomplex decreased with increasing RNA content. However, photosensitizer-attachment to TatBim and photoirradiation significantly improved the apoptotic activity of the nanocomplex by facilitating dispersion of the peptide and RNA in the cytoplasm. Combined apoptotic activity of both TatBim peptide and miR-34a in the nanocomplex was demonstrated by substituting TatBim with Lipofectamine and by substituting miR-34a with scrambled siRNA.

Original languageEnglish
Pages (from-to)110-116
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume128
Issue number1
DOIs
Publication statusPublished - Jul 2019

Keywords

  • Apoptosis
  • Cell-penetrating peptide
  • Nanocomplex
  • Photosensitizer
  • TatBim
  • miRNA

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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