Color-discriminating retinal configurations of sensory rhodopsin i by photo-irradiation solid-state NMR spectroscopy

Hiroki Yomoda, Yoshiteru Makino, Yuya Tomonaga, Tetsurou Hidaka, Izuru Kawamura, Takashi Okitsu, Akimori Wada, Yuki Sudo, Akira Naito

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

SRI (sensory rhodopsin I) can discriminate multiple colors for the attractant and repellent phototaxis. Studies aimed at revealing the color-dependent mechanism show that SRI is a challenging system not only in photobiology but also in photochemistry. During the photoreaction of SRI, an M-intermediate (attractant) transforms into a P-intermediate (repellent) by absorbing blue light. Consequently, SRI then cycles back to the G-state. The photoreactions were monitored with the 13C NMR signals of [20- 13C]retnal-SrSRI using in situ photo-irradiation solid-state NMR spectroscopy. The M-intermediate was trapped at -40 C by illumination at 520 nm. It was transformed into the P-intermediate by subsequent illumination at 365 nm. These results reveal that the G-state could be directly transformed to the P-intermediate by illumination at 365 nm. Thus, the stationary trapped M- and P-intermediates are responsible for positive and negative phototaxis, respectively. The photocycle of a sensory rhodopsin I (SrSRI) was examined using in situ photo-irradiation solid-state NMR spectroscopy. The M-intermediate was trapped by illumination (520 or 595 nm) and is responsible for the positive phototaxis. The M-intermediate was transferred to the P-intermediate (negative phototaxis) by irradiation at 365 nm. These results provide insights into the mechanism of the color-discriminating functional switch of SrSRI.

Original languageEnglish
Pages (from-to)6960-6964
Number of pages5
JournalAngewandte Chemie - International Edition
Volume53
Issue number27
DOIs
Publication statusPublished - Jul 1 2014
Externally publishedYes

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Keywords

  • biological activity
  • photo-irradiation
  • retinal chromophores
  • sensory rhodopsin I
  • solid-state NMR spectroscopy

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Medicine(all)

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