Abstract
Vessel anastomosis is important in tumor angiogenesis as well as for vascularization therapy for ischemia and other diseases. We report here the development of a color-coded imaging model that can visualize the anastomosis between blood vessels of red fluorescent protein (RFP)-expressing vessels in vascularized Gelfoam® previously transplanted into RFP transgenic mice and then re-transplanted into nestin-driven green fluorescent protein (ND-GFP) mice where nascent blood vessels express GFP. Gelfoam® was initially transplanted subcutaneously in the flank of transgenic RFP nude mice. Skin flaps were made at 14 days after transplantation of Gelfoam® to allow observation of vascularization of the Gelfoam® using confocal fluorescence imaging. The implanted Gelfoam® became highly vascularized with RFP vessels. Fourteen days after transplantation into RFP transgenic nude mice, the Gelfoam® was removed and re-transplanted into the subcutis on the flank of ND-GFP transgenic nude mice in which nascent blood vessels express GFP. Skin flaps were made and anastomosis between the GFP-expressing nascent blood vessels of ND-GFP transgenic nude mice and RFP blood vessels in the Gelfoam® was imaged 14 and 21 days after retransplantation. The results presented in this report indicate a possible mechanism for tumor angiogenesis and suggest a new paradigm of therapeutic revascularization of ischemic organs requiring new blood vessels and in other diseases.
| Original language | English |
|---|---|
| Pages (from-to) | 3041-3046 |
| Number of pages | 6 |
| Journal | Anticancer Research |
| Volume | 33 |
| Issue number | 8 |
| Publication status | Published - Aug 1 2013 |
| Externally published | Yes |
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Keywords
- Anastomosis
- Angiogenesis
- Blood vessels
- Color-coded imaging
- Confocal microscopy
- Gelfoam®
- Green fluorescent protein
- Nestin
- Red fluorescent protein
- Transgenic nude mice
- Vascularization
ASJC Scopus subject areas
- Oncology
- Cancer Research
Cite this
Color-coded imaging of spontaneous vessel anastomosis in vivo. / Uehara, Fuminari; Tome, Yasunori; Reynoso, Jose; Mii, Sumiyuki; Yano, Shuuya; Miwa, Shinji; Bouvet, Michael; Maehara, Hiroki; Kanaya, Fuminori; Moossa, Abdool R.; Hoffman, Robert M.
In: Anticancer Research, Vol. 33, No. 8, 01.08.2013, p. 3041-3046.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Color-coded imaging of spontaneous vessel anastomosis in vivo
AU - Uehara, Fuminari
AU - Tome, Yasunori
AU - Reynoso, Jose
AU - Mii, Sumiyuki
AU - Yano, Shuuya
AU - Miwa, Shinji
AU - Bouvet, Michael
AU - Maehara, Hiroki
AU - Kanaya, Fuminori
AU - Moossa, Abdool R.
AU - Hoffman, Robert M.
PY - 2013/8/1
Y1 - 2013/8/1
N2 - Vessel anastomosis is important in tumor angiogenesis as well as for vascularization therapy for ischemia and other diseases. We report here the development of a color-coded imaging model that can visualize the anastomosis between blood vessels of red fluorescent protein (RFP)-expressing vessels in vascularized Gelfoam® previously transplanted into RFP transgenic mice and then re-transplanted into nestin-driven green fluorescent protein (ND-GFP) mice where nascent blood vessels express GFP. Gelfoam® was initially transplanted subcutaneously in the flank of transgenic RFP nude mice. Skin flaps were made at 14 days after transplantation of Gelfoam® to allow observation of vascularization of the Gelfoam® using confocal fluorescence imaging. The implanted Gelfoam® became highly vascularized with RFP vessels. Fourteen days after transplantation into RFP transgenic nude mice, the Gelfoam® was removed and re-transplanted into the subcutis on the flank of ND-GFP transgenic nude mice in which nascent blood vessels express GFP. Skin flaps were made and anastomosis between the GFP-expressing nascent blood vessels of ND-GFP transgenic nude mice and RFP blood vessels in the Gelfoam® was imaged 14 and 21 days after retransplantation. The results presented in this report indicate a possible mechanism for tumor angiogenesis and suggest a new paradigm of therapeutic revascularization of ischemic organs requiring new blood vessels and in other diseases.
AB - Vessel anastomosis is important in tumor angiogenesis as well as for vascularization therapy for ischemia and other diseases. We report here the development of a color-coded imaging model that can visualize the anastomosis between blood vessels of red fluorescent protein (RFP)-expressing vessels in vascularized Gelfoam® previously transplanted into RFP transgenic mice and then re-transplanted into nestin-driven green fluorescent protein (ND-GFP) mice where nascent blood vessels express GFP. Gelfoam® was initially transplanted subcutaneously in the flank of transgenic RFP nude mice. Skin flaps were made at 14 days after transplantation of Gelfoam® to allow observation of vascularization of the Gelfoam® using confocal fluorescence imaging. The implanted Gelfoam® became highly vascularized with RFP vessels. Fourteen days after transplantation into RFP transgenic nude mice, the Gelfoam® was removed and re-transplanted into the subcutis on the flank of ND-GFP transgenic nude mice in which nascent blood vessels express GFP. Skin flaps were made and anastomosis between the GFP-expressing nascent blood vessels of ND-GFP transgenic nude mice and RFP blood vessels in the Gelfoam® was imaged 14 and 21 days after retransplantation. The results presented in this report indicate a possible mechanism for tumor angiogenesis and suggest a new paradigm of therapeutic revascularization of ischemic organs requiring new blood vessels and in other diseases.
KW - Anastomosis
KW - Angiogenesis
KW - Blood vessels
KW - Color-coded imaging
KW - Confocal microscopy
KW - Gelfoam®
KW - Green fluorescent protein
KW - Nestin
KW - Red fluorescent protein
KW - Transgenic nude mice
KW - Vascularization
UR - http://www.scopus.com/inward/record.url?scp=84883299604&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883299604&partnerID=8YFLogxK
M3 - Article
VL - 33
SP - 3041
EP - 3046
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 8
ER -