Color-coded imaging of spontaneous vessel anastomosis in vivo

Fuminari Uehara, Yasunori Tome, Jose Reynoso, Sumiyuki Mii, Shuya Yano, Shinji Miwa, Michael Bouvet, Hiroki Maehara, Fuminori Kanaya, Abdool R. Moossa, Robert M. Hoffman

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5 Citations (Scopus)

Abstract

Vessel anastomosis is important in tumor angiogenesis as well as for vascularization therapy for ischemia and other diseases. We report here the development of a color-coded imaging model that can visualize the anastomosis between blood vessels of red fluorescent protein (RFP)-expressing vessels in vascularized Gelfoam® previously transplanted into RFP transgenic mice and then re-transplanted into nestin-driven green fluorescent protein (ND-GFP) mice where nascent blood vessels express GFP. Gelfoam® was initially transplanted subcutaneously in the flank of transgenic RFP nude mice. Skin flaps were made at 14 days after transplantation of Gelfoam® to allow observation of vascularization of the Gelfoam® using confocal fluorescence imaging. The implanted Gelfoam® became highly vascularized with RFP vessels. Fourteen days after transplantation into RFP transgenic nude mice, the Gelfoam® was removed and re-transplanted into the subcutis on the flank of ND-GFP transgenic nude mice in which nascent blood vessels express GFP. Skin flaps were made and anastomosis between the GFP-expressing nascent blood vessels of ND-GFP transgenic nude mice and RFP blood vessels in the Gelfoam® was imaged 14 and 21 days after retransplantation. The results presented in this report indicate a possible mechanism for tumor angiogenesis and suggest a new paradigm of therapeutic revascularization of ischemic organs requiring new blood vessels and in other diseases.

Original languageEnglish
Pages (from-to)3041-3046
Number of pages6
JournalAnticancer research
Volume33
Issue number8
Publication statusPublished - Aug 1 2013
Externally publishedYes

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Keywords

  • Anastomosis
  • Angiogenesis
  • Blood vessels
  • Color-coded imaging
  • Confocal microscopy
  • Gelfoam®
  • Green fluorescent protein
  • Nestin
  • Red fluorescent protein
  • Transgenic nude mice
  • Vascularization

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Uehara, F., Tome, Y., Reynoso, J., Mii, S., Yano, S., Miwa, S., Bouvet, M., Maehara, H., Kanaya, F., Moossa, A. R., & Hoffman, R. M. (2013). Color-coded imaging of spontaneous vessel anastomosis in vivo. Anticancer research, 33(8), 3041-3046.