Colonial variation and fimbriation of Actinobacillus actinomycetemcomitans

Tetsuyoshi Inoue, H. Ohta, S. Kokeguchi, K. Fukui, K. Kato

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Three colonial variants of Actinobacillus actinomycetemcomitans, which formed transparent rough (TR)-, transparent smooth (TS)-, and opaque smooth (OS)-surfaced colonies, were described in relation to their fimbriation. TR- and TS-cells were adhesive to agar and glass surfaces but not the OS-cells. The examination by electron microscopy revealed that TR-cells were highly fimbriated but not TS- and OS-cells. Thus, TS-cells seemed to be an intermediate type. The fimbriae were isolated from TR-cells by suspending in 0.15 M ethanolamine-HCl buffer (pH 10.5) and purified by dissolving non-fimbrial components in 0.5% deoxycholate and 0.7% n-octyl-β-d-glucopyranoside. The relative molecular mass of the fimbrial subunit protein was 54 000.

Original languageEnglish
Pages (from-to)13-17
Number of pages5
JournalFEMS Microbiology Letters
Volume69
Issue number1-2
DOIs
Publication statusPublished - May 1990

Keywords

  • Actinobacillus actinomycetemcomitans
  • Colonial variation
  • Fimbriae

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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